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Basic Research for the Development of Fertiliser Spreaders
52-57Views:79The knowledge of the physical characteristics of fertiliser particles is essential for the constructors and operators of fertiliser distributors. Among physical characteristics, the most important are the frictional and aerodynamic properties for the description of particle movement. Adjustable angled slopes, shearing boxes and various rotating disks are used to identify frictional properties. We have developed a high precision shearing box with digital force measuring cells and a distance signaller (incremental transducer) that we use for slide tests efficiently. We measured the frictional characteristics of 6 different fertilisers: the inner coefficient of friction and the coefficient of friction on ten test surfaces most commonly used in machinery, and we specified the relationship between displacement, loading and the coefficient of friction. We can conclude that the material of the frictional surface significantly influences the force of friction.
However, our experience tells us that the shearing box is not suitable for the measurement of the inner friction, since the examined particles slide on the metal surface of the shearing box in a growing extent in the course of displacement, so it does not measure the real inner friction. Therefore, in our experiment we have developed rotating shearing equipment with a constant shearing surface to identify the inner friction. We tested the equipment with fertilisers and we identified the inner frictional characteristics of 6 different fertilisers. With the developed rotating shearing apparatus we could measure the real inner friction of the particles.
To identify the aerodynamic characteristics of granules, wind tunnels and free-fall tests are used. An elutriator have been developed for our investigation. We have used fertilisers for testing the measuring equipment and we have identified the aerodynamic characteristics of 6 different fertilisers. -
Bacteria in the milk of sheep with or without mastitis- mini Review
47-52Views:302From a nutritional point of view, sheep milk is more valuable than cow and goat milk and the interest for sheep milk is increasing in many countries. However, sheep milk is easily contaminated during milking, handling, and transport and it is an ideal medium for bacterial propagation. Consequently, sheep milk spoils quite quickly. The proper, clean handling of milk is not only of sanitarian interest, but it also serves the farmers’ interests, because contaminated milk may not be distributed, and is unsuitable for producing good quality products. Following this technological trend, this review addresses the bacterial composition of sheep milk with and without mastitis. Even though sheep milk contains a lot of bacteria, this review article highlighted total plate count, Enterobacteriaceae, coliform, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Campylobacter, Salmonella spp. and Streptococcus spp. Mastitis in sheep is a vital cause of mortality, reduction in milk production and early culling. The reported risk factors for mastitis in sheep were age, a case of mastitis, breed, husbandry systems, and location. The main priority should be implementation of programs to minimize human pathogenic bacteria and mastitis in raw ewe milk.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:204Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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The Natural, Social and Economic Conditions and Opportunities for Development of Balmazujvaros, Especially in the Case of the Co-operative Kvaliko
77-85Views:75Balmazujvaros as a settlement near Hortobagy has to cope with both advantages and disadvantages. Its natural and social conditions are mainly given, the agricultural characteristic is dominant, and the number of the employed is the highest in the agricultural firms and processing industry. Developing the third sector, including hosting, tourism, eco-tourism, thermal-tourism, may be an opportunity for the city. The subsidy for rural development relating to the SAPARD-programme may contribute to this, as the city is the member of the Association of Hajdu Towns.
The agriculture plays an important part in Balmazujvaros in which production and marketing of vegetables and fruits excel. Several farmers realised this opportunity and founded the Marketing Co-operative of Vegetable Producers in Eastern Hungary, that is the Kvaliko. This PO works in a Corporation form and is constantly developing, spreading and expanding its choice making use of the local natural, social and economic conditions and applications for realising its investments. This PO may set an example for the other entrepreneurs and farmers of Balmazujvaros to co-operate and it may be considered as an already realised opportunity. -
Penicillium chrysogenum antifungal protein (PAF) production in transgenic tobacco (Nicotiana tabacum) plant
77-82Views:74Under the „Molecular farming” research program (product vaccines and substances for medical use with gene manipulated plant) in 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture Department of Plant Biotechnology experiments were launched to transform tobacco plant by PAF antifungal protein. Our aim was to learn the transformation technics. We chose the
Nicotiana tabacum and PAF as model systems.
Our work was to express several different paf constructions in plants with nuclear and plastid transformation too. After that we confirmed the presence of paf gene in the level of DNA and RNA. -
The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:137he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Rippl-Rónai’s color in the native parks: production and using of new, synthetic Wild sage color-mixture
59-64Views:151The climate changes is becoming more damaging to ornamental plants. Besides ornamental species and varieties of plants on public spaces. It will be necessary to use domestic, well adaptable climate-tolerant plant species.
One field of our growing ornamental plants researches from 2001 in the Centre of Agricultural Sciences of University of Debrecen, is studying drought patient, mainly Hungarian improved annual varieties, which are able to get acclimatized with the landscape. Moreover, we have in view to work out new, economical seedling production technologies. In the program, the excellent drought- and frost tolerant annuals and perennials belongs to the climate change tolerant plants. From the evaluated of species we want to create and spread the application. of the new types of ornamental horticulture culture.
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Effect of different lysine-metabolized energy ratio on performance of meat-type geese
39-42Views:184The aim of the experiment was to determine, how the different ratios of the digestibility lysine/ metabolized energy (0.82, 0.87, 0.91 g DLYS/MJ AMEn) of the feed influence the performance of geese. There were 150 goslings (3 treatments, 5 cages/treatments, 10 birds/cages). The experiment has started and finished at the age of 3 and 9 weeks, respectively.
The results of the experiment showed, the different ratios of DLYS/AMEn (0.82, 0.87, 0.91 g DLYS/MJ AMEn) did not influence the performance of young geese. Better growth performances were found for the 0.91 g DLYS/AMEn feed (feed intake, body weight gain, feed and energy conversion rate). This treatment coincided with high feeding costs. Based on these results we need more models and farm experiments to prove this tendency. -
Fast neutron irradiation long term effect on Ocimum basilicum germination and initial vegetative parameters
23-31Views:98The goal of this study is to assess the long-term (3-year) effects of rapid neutron irradiation using an Americium-Beryllium source on two generations (M0 and M1) of Ocimum basilicum commercial trait. Seeds of each variety and generation were irradiated with 0, 7.5, 20, 40, and 50 Gray in 2018, and then seeded in 2021 – with their corresponding controls – to see how the different irradiation treatments affected germination and early vegetative metrics three years after irradiation. In the first generation, no significant changes between the treatments were seen. Increased irradiation dosage resulted in a significant decrease in germination % in the second generation (M1), but a significant increase in seedling height. The M0 generation seedlings, on the other hand, were substantially shorter than controls. In the M0 generation, the higher dosages (40 and 50 Gray) were deadly, and seedlings were terminated 7–10 days after germination. The irradiation dose of 20 Gray was shown to be the most successful in inducing viable and beneficial alterations for phenotypic characteristics in basil. When compared to control plants, the lower (7.5 and 20 Gray) bombardment generated leaf anomalies such as smaller leaves and internodes, a split apex, and a ribbon-like stem.
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Effect of hail net on the water potential of an apple orchard
109-113Views:129Apple production has seen a decline in yields in recent years, primarily due to ecological factors such as drought, water stress, water scarcity, uneven rainfall distribution, frost damage and hail damage. Ecological factors that are harmful to the plant can be eliminated by human intervention, irrigation or the use of hail net. In our study, we investigated the positive effects of hail net on the development of plant water potential as the vegetation progressed, in non-irrigation area with temperature and humidity. Water potential values were determined using a field osmometer. Confirmed the positive effect of the ice protection net in the apple orchard of Early Gold and Golden Reinders. Our results were supported by statistical analyzes our results.
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Productivity and Nutrient Reaction of Maize Hybrids
78-83Views:71Several factors influence the quantity and stability of maize yield, the most important being the nutrient supply, the hybrid and precipitation. In 2004, during the maize growing season the precipitation was more than the 30 year’s average, with 68.3 mm, but the distribution was unfavorable. The experiment was carried out in Debrecen at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization.
The favorable results reached were due to the rainy season. The average yield varied between 7.78-9.67 tha-1. The DK 440, PR37M34, PR38A24, PR39D81 and PR36R10 of the hybrids reacted to higher fertilizer doses with significant growth yields, the yield (more than 11 tha-1) was the highest for N200, P125, K150 fertilizing. The other hybrids, DKC 5211, Mv Vilma and MV Maraton, gave similar results at the N120+PK fertilizer dose and the ensuing doses depressed the yield. Fertilization was more effective thanks to the precipitation. The fourth and fifth dose increased or decreased maize yield depending on the nutrient reaction of the hybrid. The agro-ecological optimum of NPK fertilization was N120, P75, K90 kgha-1.
During the experiment we tested the moisture loss of five hybrids. The rainy crop year’s effect on the seed moisture content at harvest was higher than in previous years. The seed moisture content hybrids at harvest which have shorter crop years (FAO 300) was 18-19%, and hybrids with longer vegetation periods had more than 20% seed moisture content. DK 440 hybrid had the intensive moisture loss of the five hybrids, at the start of the measurement, the seed moisture content was higher than 40%, and it decreased to 18.6% by harvesting. -
Opportunities for improving the nutritional value of cereal-based products
275-278Views:158We set up experiments for improving the nutritional value of cerealbased products. Our aims were to decrease the energy content of bakery products with the mixing of plant originated raw materials and byproducts with high fibre contents, and we have evaluated the effect of sodium-chloride on the physical properties of bakery products. We found that the apple pomace, the byproduct of juice production, is excellent for increasing of fibre content of bakery products and the further aim of investigation is to develop economical ways of hygienic byproduct handling and purification. Our experiments, evaluate the bakery use of triticale, have significant achievements and the breads made from triticale flour and whole-grain are commercially available nowadays. Our investigations included the possibilities of decrease of toxin contamination of cereals and our results can form a part of risk estimation systems after further experiments .
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HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:239Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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Growth and yield patterns of black locust (Robinia pseudoacacia L.) sample trees affected by site conditions: case studies
125-128Views:30The trees removed from the long-term experiment plots are available for the measurements as lying trees. Through the determination of the volume in sections along the stem, the stem form, the stem volume and other factors can be specified. The comparison of the stems of individual trees of first and third yield classes of black locust (Robinia pseudoacacia L.) stands shows that site conditions have a main effect on the yield (mean tree volume). The difference can be as high as 53% at the age of 30 depending on the sites. To determine the growth patterns based on tree volume is rather a new approach in the light of the relevant literature. The obtained results also highlight the importance of choosing the appropriate tree species for a given site.
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Advancement of a Common Wheat (Triticum aestivum L.) Selection System
8-11Views:84All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect. -
Studying on the frost damage of some peach and nectarine varieties
13-15Views:83To be able to grow stone fruit species, the peach and the nectarine varieties, yield stability is very relevant. However, the climate is suitable in Hungary for growing stone fruits. We have to calculate with damage. Important winter damage and spring frost damage occur in every second year on the Great Pplains. To reveal the frost tolerance of cultivars and to clarify the differences among growing areas is the most important point of peach and nectarine production.
We, made our experiments in the winter of 2005 (January-February), at Pallag, Zsombó, Siófok, Sóskút and Szatymaz. These former places are determinant in peach and nectarine growing in Hungarian respects.
In our experiments we analyzed 10-10 fruit shoot from 82 varieties, and we measured the frost damage and the flower bud density.
The minimum and the maximum frost damage values (%) at the different growing places were 7.6-38.7 at Siófok, 6.5-31.3 at Sóskút, 34.3-100 at Szatymaz, 83.7-99.6 at Pallag and 51.9-99.6 at Zsombó. There were expressive differences between the same cultivars depending on the growing area.
The frost damage of „Suncrest” variety was 7.6% at Siófok, 8.2% at Sóskút but it was 69.8% at Szatymaz. The „Maystar” variety had 17.6% at Siófok, 12.4% at Sóskút and 87.2% frost damage at Szatymaz. In case of „Redhaven” variety the frost damage values were 16.8% at Sóskút, 54.6% at Szatymaz. We collected fruit shoots from three different heights in case of „Cresthaven” variety. In case of 1 meter height the frost damage was 74.6%, at 2 meter height 44.7%, and 25.2% at 3 meter.
We experienced also big differences in flower bud density (flower bud/10 cm) of varieties. The following cultivars had the highest flower bud density (6-7 bud/10 cm): „Suncrest”, „Meystar, „Maria Luisa” and the „Maria Bianca”, „Redhaven”, „Michellini” had the lowest flower bud density (2-3 bud/10 cm).
After our experiments we established that there were very expressive differences among the frost damage of peach and nectarine varieties from different climatic conditions and there are also big variance between the frost damage values of those fruit shoots, what were collected from different heights, but from the same variety. It conspicuous very good among our items, that difference of the flower bud density is very big among the varieties.To be able to grow peach and nectarine successfully, the suitable variety (low frost damage value, high flower bud density) and the suitable growing are is determinant. -
The effect of different bacterial fertilizers on the AL-soluble P2O5 content of soil, and the biomass of the rye-grass (Lolium perenne, L)
93-98Views:186In pot experiment the effect of different bacterial fertilizers on some soil properties, and the amount of plant biomass were studied. The
experiment was set up in 2010 at the Department of Soil Science and Agricultural Chemistry, in a three replications in a random block design. The ryegrass (Lolium perenne, L.) was used as a test plant. The studied soil type was calcareous chernosem soil from Látókép. In our laboratory AL-soluble P2O5 content of soil, the phosphatase enzyme activityof soil, the dry weight of rye-grass, and the phosphorus content of rye-grass were determined.
The results of the study were the following:
– The bacterial fertilizers - by basic treatments NPK - had significant positive effect on the AL- soluble phosphorus content of the soil.
– The soil phosphatase enzyme activity was increased in all cases strongly by the microbial preparations used, the greatest impact was the Bactofil A bacterial fertilizer.
– The plant educed P values significantly increased by the effect of microbial products, in addition to the fund NPK. In this case, the EM-1 and Microbion UNC bacterial fertilizer were the effective.
– In case of the rye-grass biomass none of the bacterial preparations used caused any significant changes, either alone or when used them with straw treatment. -
Studies on the development of food attractants catching noctuid mouths (Lepidoptera: Noctuidae)
86-91Views:150The monitoring of the most dangerous noctuid pests (Lepidoptera: Noctuidae) can be performed by species specific pheromone traps. Recently the development of traps catching female moths became the main objective of the studies. We studied the synergistic effect of vine and beer as natural additive on the effectiveness of baits containing isoamyl alcohol and acetic acid in Forró and Debrecen-Ondód. The addition of vine and beer had positive effect on catches and it was significant in case of two dangerous pest species Agrotis segetum and Lacanobia oleracea. The synergistic effect was also significant with regard to the number of detected species especially in case of lower abundances and rare species.
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Visualization techniques in agriculture
95-98Views:116This paper describes a dynamic map representation method which provides a flexible, spectacular and cost-effective opportunity for the illustration and description of spatial data due to its parametrability, web-based publication and the free sowftare it uses in multi-user circumstances.
The tasks of the database serves and the processing were performed by an ASUS WL-500 G Premium v2 router and a 80 GB hard disk. The database contains the measured data of the nitrogen fertilisation experiment established on the Látókép Experiment Site of the Centre for Agricultural Sciences of the University of Debrecen. The tests showed that the generation time of the processor which was run through the router did not significantly increase. Therefore, the configuration developed by us is suitable for users who do not wish to invest into a large and expensive server, but they still want to view their data quickly and easily, as well as to reach them from anywhere.
The available data were not sorted into a database which was performed with Quantum GIS in a way to have an optimum database structure which is adjusted to the expected areas of use and the expected running speeds were also taken into consideration. The processor which processes the database items was written in PHP language. The main role of the processor is that it produces a KML file real time which is suitable for viewing with a given map viewer client (e.g. Google Earth). This application makes it possible to view information related to geographical objects, values stored in the database or those calculated by the processor on a map in 2D or 3D in a versatile way.
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Long-term effect of soil management on the carbon-dioxide emission of the soil
515-527Views:81CO2 emission from soils is one of the most important elements of the global carbon cycle, thus it has crucial rule in climate change. Each soil cultivation operation intervenes in the microbiological life of the soil, hence tillage is a factor through that the processes taking place in soil can be controlled. During the last decades, the organic material content of agricultural soils decreased to the half due to the intensive management resulting in the degradation of natural soil fertility. While intensive, plough-based tillage can cause soil degradation and erosion, the physical, chemical and biological status of the soil can be significantly improved through the application of conservation tillage methods. The results of long-term experiments prove that soil protective tillage enhances the enrichment of organic matter in the top layer of the soil. In order to reveal the role of tillage systems in CO2 emission from the soil, regular measurements were carried out in the plots with conventional and reduced tillage of the soil cultivation experiment of Research Institute of Karcag. Anagas CD 98 and Gas Alert Micro 5w infrared gas analysers were used to measure CO2-concentrations, and a specially developed method (consisting of a frame and a bowl) was applied to delimitate the measuring area. Most of the measurements were done on stubbles after harvest in order to exclude root respiration. The weather conditions of the examined 10 years were very changeable providing a good chance to compare them to each other. We found the tillage operations resulting in higher emission values in both tillage systems. On stubbles higher and more even emission was characteristic to reduced tillage due to the lower degree of soil disturbance and higher soil moisture content.
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Strategic planning in agribusiness
23-27Views:126Agricultural businesses operate in a complex and dynamic environment, with many challenges and opportunities. It is therefore essential for these businesses to have a strategic plan. It is a critical process that helps businesses navigate in a complex and uncertain environment and achieve long-term goals and objectives. In this article, we have conducted a bibliometric analysis of academic journals to investigate the extent to which strategic planning is a researched topic in agribusiness enterprises. We found that there is no concentrated research, with only three keywords appearing in the literature with at least five repetitions. Even of the two repetitions, only 22 were found. The clustering of keywords helps to identify research directions. The results of a survey of 134 enterprises were then presented. The majority of enterprises do not have a long-term plan or even a written vision. We found that SWOT analysis is still the most common planning method among the companies surveyed. It was also found that there is no difference between industries in this respect. Significant differences were only found in relation to company size, with all large companies having a strategic plan and the vast majority of SMEs not. Only 17 out of 132 firms have a plan longer than three years, and 32 do not have any plan at all. Strategic planning can help to address many of the challenges in the agribusiness area, and it is therefore proposed to improve the proportion of firms planning through knowledge transfer.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:202The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:91Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:130Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Effect of selenium supplement on proteome of chicken liver
9-13Views:137The aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.