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The effect of different bacterial fertilizers on the AL-soluble P2O5 content of soil, and the biomass of the rye-grass (Lolium perenne, L)
93-98Views:186In pot experiment the effect of different bacterial fertilizers on some soil properties, and the amount of plant biomass were studied. The
experiment was set up in 2010 at the Department of Soil Science and Agricultural Chemistry, in a three replications in a random block design. The ryegrass (Lolium perenne, L.) was used as a test plant. The studied soil type was calcareous chernosem soil from Látókép. In our laboratory AL-soluble P2O5 content of soil, the phosphatase enzyme activityof soil, the dry weight of rye-grass, and the phosphorus content of rye-grass were determined.
The results of the study were the following:
– The bacterial fertilizers - by basic treatments NPK - had significant positive effect on the AL- soluble phosphorus content of the soil.
– The soil phosphatase enzyme activity was increased in all cases strongly by the microbial preparations used, the greatest impact was the Bactofil A bacterial fertilizer.
– The plant educed P values significantly increased by the effect of microbial products, in addition to the fund NPK. In this case, the EM-1 and Microbion UNC bacterial fertilizer were the effective.
– In case of the rye-grass biomass none of the bacterial preparations used caused any significant changes, either alone or when used them with straw treatment. -
Studies on the development of food attractants catching noctuid mouths (Lepidoptera: Noctuidae)
86-91Views:150The monitoring of the most dangerous noctuid pests (Lepidoptera: Noctuidae) can be performed by species specific pheromone traps. Recently the development of traps catching female moths became the main objective of the studies. We studied the synergistic effect of vine and beer as natural additive on the effectiveness of baits containing isoamyl alcohol and acetic acid in Forró and Debrecen-Ondód. The addition of vine and beer had positive effect on catches and it was significant in case of two dangerous pest species Agrotis segetum and Lacanobia oleracea. The synergistic effect was also significant with regard to the number of detected species especially in case of lower abundances and rare species.
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HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:239Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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Strategic planning in agribusiness
23-27Views:126Agricultural businesses operate in a complex and dynamic environment, with many challenges and opportunities. It is therefore essential for these businesses to have a strategic plan. It is a critical process that helps businesses navigate in a complex and uncertain environment and achieve long-term goals and objectives. In this article, we have conducted a bibliometric analysis of academic journals to investigate the extent to which strategic planning is a researched topic in agribusiness enterprises. We found that there is no concentrated research, with only three keywords appearing in the literature with at least five repetitions. Even of the two repetitions, only 22 were found. The clustering of keywords helps to identify research directions. The results of a survey of 134 enterprises were then presented. The majority of enterprises do not have a long-term plan or even a written vision. We found that SWOT analysis is still the most common planning method among the companies surveyed. It was also found that there is no difference between industries in this respect. Significant differences were only found in relation to company size, with all large companies having a strategic plan and the vast majority of SMEs not. Only 17 out of 132 firms have a plan longer than three years, and 32 do not have any plan at all. Strategic planning can help to address many of the challenges in the agribusiness area, and it is therefore proposed to improve the proportion of firms planning through knowledge transfer.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:201The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:91Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:130Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Effect of selenium supplement on proteome of chicken liver
9-13Views:137The aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.
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Investigation of new seed mixtures in wildlife forage ground managem
9-13Views:169One of the most important method of habitat development is wildlife forage ground management. Many plant species can be grown on the crop fields, as mixture, too. Use of seed mixtures has not been spread on the native wildlife forage ground, however there are many advantages of them.
The aim of the research is to examine the role of the different plant species, seed mixtures in the game feeding, the savouriness of the plants of crop fields, as well as ascertain which seed mixtures are more suitable for wildlife forage ground management. On the basis of the investigation it can be find out that big amount of forage can be produced on mixed crop fields. Buckwheat, corn, rape, millet and white lupine proved to be the most beloved plants. It is suitable to make such mixed forages and use widely in the native wildlife forage ground management,
– which assures better feed conditions for the game,
– which are more attractive for the game and can draw away from other areas, so prevent, reduce game damage in forestry sector and in
agriculture that is in the forest manager’s, agricultural and game manager’s interests, too.
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The role of some elicitors in inducing chilling stress resistance in apricot fruit
27-32Views:139This study aimed to study the role of 2 mM salicylic acid and 0.2 mM methyl Jasmonic acid in reducing the chilling injuries on two different apricot varieties fruits (Bergarauge and Flavor cot). These treatments were applied for apricot fruit directly after harvest after measuring the zero time data. Then the fruit were stored at 1 oC for three weeks and were examined 7 day periodically, other group of fruit was stored at 1 oC for 15 days then transport to room temperature and examined after 4 and 8 days. The fruit samples were collected at each examination time, and the measurements containing the fruit firmness (Kg cm-2), chilling and decay index, membrane electrolyte leakage, total phenol content and phenylalanine ammonia lyase mechanism (PAL) activity. The results showed that there are no obvious differences between the tested varieties and the differences may back to genetic variations and they took the same trend for most of measurement after treated with SA and MJ. SA and/or MJ treated fruit achieved low percentage of fruit softening in comparison to water treated fruit. At the same time the chilling and decay index showed that the SA and/or MJ reduced the decay or breakdown symptoms in either tested varieties. The treated fruit with those elicitor help the fruit to keep about stable phenol content while water treated fruit lose the phenol content sharply at early storage and this stable level of phenol my because of the ability of those chemical to raise PAL activity in treated fruit.
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Visualization techniques in agriculture
95-98Views:116This paper describes a dynamic map representation method which provides a flexible, spectacular and cost-effective opportunity for the illustration and description of spatial data due to its parametrability, web-based publication and the free sowftare it uses in multi-user circumstances.
The tasks of the database serves and the processing were performed by an ASUS WL-500 G Premium v2 router and a 80 GB hard disk. The database contains the measured data of the nitrogen fertilisation experiment established on the Látókép Experiment Site of the Centre for Agricultural Sciences of the University of Debrecen. The tests showed that the generation time of the processor which was run through the router did not significantly increase. Therefore, the configuration developed by us is suitable for users who do not wish to invest into a large and expensive server, but they still want to view their data quickly and easily, as well as to reach them from anywhere.
The available data were not sorted into a database which was performed with Quantum GIS in a way to have an optimum database structure which is adjusted to the expected areas of use and the expected running speeds were also taken into consideration. The processor which processes the database items was written in PHP language. The main role of the processor is that it produces a KML file real time which is suitable for viewing with a given map viewer client (e.g. Google Earth). This application makes it possible to view information related to geographical objects, values stored in the database or those calculated by the processor on a map in 2D or 3D in a versatile way.
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Long-term effect of soil management on the carbon-dioxide emission of the soil
515-527Views:81CO2 emission from soils is one of the most important elements of the global carbon cycle, thus it has crucial rule in climate change. Each soil cultivation operation intervenes in the microbiological life of the soil, hence tillage is a factor through that the processes taking place in soil can be controlled. During the last decades, the organic material content of agricultural soils decreased to the half due to the intensive management resulting in the degradation of natural soil fertility. While intensive, plough-based tillage can cause soil degradation and erosion, the physical, chemical and biological status of the soil can be significantly improved through the application of conservation tillage methods. The results of long-term experiments prove that soil protective tillage enhances the enrichment of organic matter in the top layer of the soil. In order to reveal the role of tillage systems in CO2 emission from the soil, regular measurements were carried out in the plots with conventional and reduced tillage of the soil cultivation experiment of Research Institute of Karcag. Anagas CD 98 and Gas Alert Micro 5w infrared gas analysers were used to measure CO2-concentrations, and a specially developed method (consisting of a frame and a bowl) was applied to delimitate the measuring area. Most of the measurements were done on stubbles after harvest in order to exclude root respiration. The weather conditions of the examined 10 years were very changeable providing a good chance to compare them to each other. We found the tillage operations resulting in higher emission values in both tillage systems. On stubbles higher and more even emission was characteristic to reduced tillage due to the lower degree of soil disturbance and higher soil moisture content.
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Botanical Survey of a Seminatural Saline Meadow
38-44Views:71We made a botanical survey of a seminatural meadow situated in the vicinity of Hajdúnánás-Tedej in Hungary, using traditional phytocenological methods. The habitat, based on its soil-, and botanical characteristics, can be classified into the category of saline meadows. One of the landscape ecological values of this area is its mosaic-like structure and, as a consequence, this habitat can be divided into six characteristic associations with blurry borderlines and characteristic overlaps. Because of its rare plant species and species richness this area can be considered to be a valuable „seed bank” and a „stepping stone” in the ecological network among the larger, intensively cultivated fields. The area can be evaluated as moderately recovered, the natural species are dominant, but there are few disturbance tolerant species. Except for the two saline associations, the high grass associations, which make up about two-thirds of the habitat, are cut once a year; therefore, the agricultural utilisation of the area and its prevention against weediness are being assured at the same time.
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Study of effects on quality of different wheat flour fraction
123-129Views:96Wheat is one of the most important cereals in the world and the bread made of its flour belongs to the everyday life of human mankind.
The Hungarian standard relating to the laboratory production of wheat flour (MSZ 6367/9-1989) does not mention the type of laboratory mill used for milling, and it only builds up some general criteria, such as: the laboratory mill should be provided with four differently nicked barrels, a sieve with appropriate hole sizes, and also with the separated collections of the pilot flour and the bran. Our study was started at this point and the answers for the following questions were aimed to be found: do the flour patterns studied and produced with different grinding and sieving
techniques, widely used in laboratory mills of the same wheat pattern show any alterations after the impact of the formula production as regards chemical constitutions and reologic parameters. Various flours and whole grains of the wheat patterns sieved with different particle sizes were studied in this experiment. In producing this pattern two different mill types of FQC 109 and CHOPIN CD 1 as well as two different grinder types such as PERTEN 3100 and type of RETSCH 200 were applied. There were 3 different corn sizes of 160; 250; 800 μms used in the partition of the fractions. To study the differences the following measurements were conducted: dry matter, ash, protein content, wet gluten content, gluten index, gluten expansiveness, farinographic value, falling number and amilographic rate.
The results this research confirm that the quality of wheat flour can be modified by different methods of pattern production. In all cases the differences can be explained by the flour-bran ratio, and in some of the cases the higher germ content of the fractions also played a role. The results show differences between the various types of mills and grinders, too. -
The effect of short term storage on different winter wheat varieties rheological properties
83-86Views:153The aim of storage after harvest is to protect the quality of wheat, because after-ripening occurs in the first 5–6 weeks. During this time it very important to make the optimal storage conditions.
We have carried out storage experiment with wheat samples from Látókép Research Farm of the University of Debrecen. We analyzed the rheological parameters of Lupus and GK Csillag varieties from the crop year 2011/2012. The experiment period was between July and August 2012 (24. 07. 2012., 31. 07. 2012., 21. 08. 2012.).We determined the rheological parameters (water absorption, dough stability time and valorigraph quality number) of Lupus and GK Csillag during short term storage. Our results showed that after-ripening increased the baking quality of Lupus and GK Csillag during storage.
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The possibility of use of the 0,01 M CaCl2 and Baker- Amacher extractants for the determination of plantavailable potassium
7-15Views:71The Hungarian fertilizing recommendation systems use AL soil test for the evaluation of potassium supply. The 0.01 M CaCl2 is a definitely milder extractant, it extracts the easily soluble and exchangeable potassium amount. Its European introduction was already taken into consideration in 1994. The research project on this topic is started in several european countries, also in Hungary at the Department of Agricultural Chemisty of Agricultural University of Debrecen. Another advantage this multielement method is that the different element-ratios can also be calculated.
The Baker-Amacher extractant’s principle is that it contains a known amount of K, P, Mg in the CaCl2 solution. During the soil extraction adsorption and desorption process take place, so the adsorption or desorption can be calculated from the original and the final concentrations.
In this paper we introduce the results of comparing analysis of the samples (n=630) from Soil Information and Monitoring System. Our aim was to measure the use of new extractants beside conventional extractant (AL) for the evaluation of K-supply would be reasonable.
It can be stated that there is a medium close relationship (r=0.75) between AL-K and 0.01 M CaCl2-K. My calculations confirmed the results of former examinations, and proved that the two extractants don’t extract and change the same rate of K-fractions. We found that regression between 0.01 M CaCl2 and AL depend on texture classes, pH classes, amount of lime, and organic matter content of soils.
Comparing the relations between AL and Baker-Amacher we find relatively loose correlation (r=0.45). We stated that there are K-fixing soils among soils considered to be well supplied with potassium by AL. This might be caused by the high amount of mineral clay and the quality of mineral clay. We stated that the dK averages show that the Hungarian nutrient-supply categories characterize generally well K-supplement of soil.
It can be stated that it would be necessary to use new extractants to specify evaluation of plant available K. We found that the 0.01 M CaCl2 and Baker-Amacher extractants could complete usefully the AL procedure and could help effective potassium fertilization. -
Study of animal welfare status in dairy cow herds in Hungary – looking for causes of lameness
47-50Views:154In the last 20–30 years lameness in cattle was found to be third the most influential disease next to mastitis and reproduction disorders. Studies have been established to explore reasons for lameness and prevention. The problem with more robust prevention plans is that knowledge and research evidence is not strong enough to run an effective prevention plan. The aim of the research is to look for reasons of lameness by observing number of cows on 6 farms during 2 lactations. Performance data will be put together to body condition score (BCS) and lameness scores. Other examination is focused on monitoring of 40 farms. This part of the project is more related to extension, collecting and sharing solutions for decreasing lameness. Producers are advised what kind of measures are possible to reduce occurrence of lameness. Effectiveness of those actions will be measured at the end of the study. The first preliminary results show lack in almost all preventive measures needed to be taken in minimising lameness. Those areas are related to poor facilities, lack of straw, problems with labour and basic management.
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Population date of Great Cormorant at the area of the Hortobágy Fish Farm Co.
51-54Views:223Hungary’s great cormorant stock did not use to be a considerable one before the 1980s. After that, their number has been rising significantly. The size of the stock now is rather stagnating or slightly growing. The presence of cormorants has been significant around ponds of the Hortobágy Fish Farm in the past few years. Due to considerable fish consumption of cormorants it is essential to know which and what kinds of units of the ponds (spawning, supply, and market) are preferred by the birds in order to see to adequate protection. The aim of our research was to discover any differences in the number of cormorants present at particular units of the fishpond system and certain ponds of equal kinds within them.
Our observations were in line with the methods used in ornithological researches. The experiment took place between April and October 2012 on a monthly basis. According to the results we found relevant differences in the number of cormorants based on the seasons and months as well. This fact was supported by statistical data. During the autumn and winter birds prefer supply and market purpose ponds while their presence here is vanishing during the summer. Relevant presence of cormorants around the area of the Old Fishponds (70 to 200 specimens) might be due to lack of disturbance and large sizes of the ponds.
Our research has great scientific relevance since it is vital to be aware of the preferred periods and areas in order to maintain effective protection; this way alerting activities can be applied in a more harmonized way. By keeping our research up as a monitoring activity, we will be able to receive more precise data later on.
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Overview of higher education funding models
129-133Views:104t can be defined in the course of the review of the financing characteristics of higher education that every model has its advantageous feature which is missing from the others, at the same time; it has its weaknesses as well. None of the basic models can meet the complex requirements of the nowadays society and government, thus the choice between the models have to be based on the preferences of decisionmakers and society. In most countries the combination of models are implemented, which compensate the weaknesses of the basic models, however, the models formed by the combination of different principles are neither perfect.
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Coincidences between molecular genetic and studbook data of gidrán mare families on the basis of mtDnA
69-73Views:157The traditional Hungarian horse breed, Gidran has been close to the edge of extinction several times. Despite the multiple bottleneck effect, the breed has retained a part of its genetic variability, and performed prominently in carriage driving and show-jumping competitions. Maintaining of the Gidran breed is important in the point of view of world heritage; because besides Hungary, smaller Gidran populations exist only in Bulgaria and Romania. Taking advantage of the special inheritance features of mtDNA, our study focused on two mtDNA regions of Gidran mares. Altogether, 251 hair samples from various Hungarian studs were examined. The analysis was successfully made in case of 251 samples of the cytochrome b and in case of 246 samples of D-loop regions. Because of the distinct mutation rates of the two mtDNA markers, the number of the haplotypes and the way of grouping samples into haplotypes was different. Our key finding was that most haplotypes may be compatible with mare families of the stud book; however incidental mistakes in stud book have occurred only in a few cases. Our results indicate the importance of the preservation and breeding those mare families, which are molecular genetically more diverse than the others, and are in the edge of extinction.
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Susceptibility of maize hybrids to corn smut diseas
39-42Views:142Corn smut disease is one of the most common maize diseases. In the previous years the disease lost from its importance, however nowadays it is becoming more and more important and widespread. The protection against it encounters great difficulties, therefore it is important to emphasize prevention and resistance based protection. The aim of the research was to investigate under laboratory circumstances the resistance of Hungary’s most commonly cultivated maize hybrids to corn smut disease. Twelve species were investigated. We distinguished two susceptibility groups within the hybrids based on the investigated parameters. The results are intended to assist the protection against corn smut disease and to make a suggestion for the farmers so that they could apply more environmental friendly and economical production.
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Investment funding programs on dairy farms in Hajdú-Bihar County
11-16Views:166The dairy farming sector needs constant modernization of its buildings, machines and technology in order to maintain its competitiveness and productivity. For the greater scale investments, these companies try to apply for state funds in addition to their own financial sources. Therefore, the investment support system plays a great role by improving the competitiveness and modernity of these farms. In the last 10 years, 7 funding programs were available for dairy farms. In this research, 25 dairy farms were interviewed in Hajdú-Bihar County. The examined farms cover more than 50% of the milk production in Hajdú-Bihar County. The survey questions focused on the investment funding program use of farms between 2007 and 2017 and renewable energy investments including all programs. The results show how many tenders were submitted, how many were successfully evaluated and completed. All farms had at least two successfully evaluated projects. A correlation was found between completed projects by farm size and completed projects. The connection of the modernity index of dairy farms and the completed projects was also shown.
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Leaf reflectance characteristics and yield of spring oat varieties as influenced by varietal divergences and nutritional supply
29-34Views:84Inadequacy of nutrients in the soil and sufficient inaccessibility to nutrients is caused by factors that affect production and productivity of spring oat varieties. Exogenous application of nutrient and real time nutrient assessment can therefore reverse these associated negative consequences. A field experiment was conducted to evaluate the response of spring oat varieties to sulphur and silicon based fertilisation. Eight spring oat varieties, four level of nutrient application was arranged in a split-plot design with three replications. The obtained results showed that foliar application of sulphur improves the grain yield of most nutrients responsive varieties by about 34.7%. However, application of silicon had shown a diminishing return association to grain yield of variety GK Kormorán, GK Pillangó, Lota, Panni. LAI, thousands grain weight, SPAD, NDVI was significantly (p < 0.05) influenced by genetic difference of the tested varieties, developmental plasticity, and nutrient application. Significantly higher grain yield was obtained from the variety Mv Pehely than the other tested varieties. Therefore, it could be inferred that a combined use of nutrient responsive spring oat varieties and sulphur containing fertilisers could be important agronomic practice to improve grain yield and to develop climate resilient oat varieties.
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Determining elements of variety-specific maize production technology
157-161Views:62Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:108Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested.