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Analysis of vitamin E content in pilot dairy products
49-52Views:158Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount.
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Microbiological status of bulk tank milk and different flavored gomolya cheeses produced by a milk producing and processing plant
73-78Views:228The microbiological quality of milk is important not only for food safety, but it can also influence the quality of dairy products. In this study, our aim was to assess the microbiological status of the bulk milk of a milk-producing farm, and some natural and flavored (garlic, dill, onion) gomolya cheeses made from pasteurized milk produced by their own processing plant. We determined the number of coliform bacteria, Escherichia coli, Staphylococcus aureus, and molds of three milk and eight cheese samples. The tests were conducted between July and September, 2017.
In bulk milk, the mean coliform count was 3.83±0.17 log10 CFU/ml; the mean E. coli count was 1.38±0.14 log10 CFU/ml; the mean mold count was 3.74±1.30 log10 CFU/ml; and the S. aureus count was <1.00 log10 CFU/ml, respectively. The mean coliform count in gomolya cheeses was 3.69±1.00 log10 CFU/g; the mean E. coli count was 2.63±0.58 log10 CFU/g; the mean S. aureus count was 3.69±1.35 log10 CFU/g and the mean mold count was 1.74±0.37 log10 CFU/g. The amount of coliforms detected in different flavored gomolya cheeses were significantly different (P<0.05). More than 10 CFU/g of E. coli was found only in the dill flavored cheeses, and S. aureus was found only in dill (3.66±1.86 log10 CFU/g) and onion (3.71±0.52 log10 CFU/g) flavored gomolya cheeses. Based on the obtained results, it was found that the amount of coliform bacteria and E. coli in bulk milk exceeded the limit set in regulation of the Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.) and the amount of S. aureus was below the limit. For gomolya cheeses, the S. aureus count exceeded the limit. The amount of coliform bacteria remained above the limit in cheeses, except for the garlic flavored gomolya cheese. In cheeses, a larger E. coli count was detected than in the bulk milk, but there is no specific limit for cheeses in the regulation. The mold count exceeded the limit specified in the regulation in cheeses, but a lower value was detected relative to milk.
The results show that, in the case of bulk milk and gomolya cheeses, certain detected quantities exceeded the limit values set forth in regulation of MoH 4/1998 (XI. 11.). The results indicate an inadequate microbiological state of the raw material and the finished products. The reasons for these are due to reduced technological hygiene or the inappropriate handling of raw material and finished products. In this study, we have summarized the results of our preliminary studies, which can provide a basis for further hygiene studies.
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Effect of feeding linseed on the fatty acid composition of milk
45-50Views:213In the last decades many researches were made to change the animal product food’s composition. The production of better fat-compound milk and dairy products became a goal in the name of health conscious nutrition. These researches were motivated by the non adequate milk fat’s fatty acid composition. There have been made researches in order to modify the milk’s fatty acids’ composition to reach the expectations of functional foods. With the optimal supplement of the feed can be increased the proportion of the polyunsaturated fatty acids and can decreased the saturated fatty acids. Row fat content of milk was not decreasing in the course of examination neither of the cold extruded linseed nor the whole linseed supplement as opposed to observations experienced by other authors. In case of monounsaturated and polyunsaturated fatty acids when supplementing with cold extruded linseed the most significant change was observable in the concentration of the elaidic acid, oleic acid, linoleic acid, alfa-linolenic acid, conjugated linoleic acid. In case of saturated fatty acids the quantity of palmitic acid and myristic acid lowered considerably. When observating the feeding with whole linseed the concentration of many fatty acids from the milkfat of saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, alfa-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers as well. The producing of milk with favourable fatty acid content from human health point of view can give scope propagate the products of animal origin. -
The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:173Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
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Comparative analysis of Carpathian Braunvieh’s morphological traits
15-19Views:176The Carpathian Braunvieh cattle established by the cross-breeding of the Schweizer Braunvieh and the local breeds of the Carpathian basin approximately 150 years ago. The evolved three usage breed was durable and resistant, however in comparison with the high-productivity breeds was less competitive. The dramatic lay-off, and the endangered status of the breed requires a conservation programme. University of Debrecen took the investigation of the Hungarian population and it’s detailed genetic studies. Within this research project among others we carry out body measurements and rates of the body traits. Depending on these results, we may decide on the subsequent gene-reserving objects. Based on body measurements and live weight we established that the breed at Mikóháza favours to the one lived in Hungary in the 1960’s, and to another, named Ukrainian Carpathian Brown. Compared to the earlier data (Horváth, 1966) smaller body traits can be seen. Our livestock is proportionately smaller than the other breeds in height at withers, as well as in live weight. Based on the comparative analysis, we established, that – thanks to the aware sorting – the imported individuals represent the ancient, primitive Carpathian variant.
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The effect of grazing of various cattle breeds on botanical composition of low-lying pasture in Hortobágy
57-63Views:161Coenological surveys were conducted in the Hortobágy National Park (Pap-ere and Zám-puszta) in May 2015 and 2016. During the tests,a total of 40 permanent plots were analyzed on grasslands grazed by extensive cattle (Hungarian Grey) and mixed genotype intensive cattle. The presence of plant species, percentages of total coverage of species and vegetation cover were recorded. Two habitat types were chosen according to their moisture content: wet salt marsh meadow (Bolboschoenetum maritimi) and drier salt meadows (Beckmannion eruciformis).
We compared the impact of increased number of animals (2016 years) and the low number of animals (2015 years, initial state) and the grazing exclusion on vegetations.
We tested: (i) what is the impact of grazing on the vegetation, (ii) how do species composition and vegetation charachteristics differ in the two habitat types (iii) and is there a difference in the impact of different cattle breeds (Hungarian gray, intensive beef cattle) grazing on the grasslands species composition? During the investigation we found, (i) that the greatest number of species was recorded in 2015, on the area that received moderate to intensive grazing (14.3 species per m2). Somewhat the number of species was reduced in 2016 due to more intensive grazing. The control group had the lowest number of species (11.7 species per m2). The undergrass and legumes cover significantly increased on intensive grazed lands. (ii) Our results indicate that the effects of different grazing differ in the two studied habitat types. On the drier grasslands greater number of species were found (16.2 species per m2), oppositely to the wet grassland (11.2 species per m2). The cover of the undergrasses was higher in the drier habitat than in the wet. (iii) The extensive beef cattle left a bigger number of species (16 species per m2) than the intensive beef cattle (11.4 species per m2). The grass cover was more intense on areas grazed by intensive cattle. The absolute and potential weeds cover showed a higher value on areas grazed by Hungarian Grey. Our two-year results suggest that grazing by both extensive and intensive cattle breeds can be a proper tool for the conservation management of alkali grasslands. -
Possibility of oil seeds in feeding dairy cows
67-73Views:147The efforts to modify the fatty acid composition of milk have intensified with health conscious nutrition coming to the forefront.This experiment of ours was designed to investigate to what extent the natural-based feed additives, such as oilseeds, can influence the fatty acid composition of cow’s milk.Further information was gained about feeding of oilseeds in specific amounts to be fitted into the technology of a large-scale dairy farm in practice. The feed supplements were whole, untreated rapeseed and whole, untreated linseed, as part of a total mixed ration. In case of saturated fatty acids when supplementing with whole rapeseed the most significant change was observable in the concentration of the caprylic acid, capric acid, undecylic acid, lauric acid, myristic acid, stearic acid. In case of unsaturated fatty acids the quantity of oleic acid enhanced considerably. When observating the feeding with whole linseed the concentration of many saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, α-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers, as well.
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Origin, history and utilization possibilities of Carpathian Braunvieh at Hungary
15-20Views:166The Carpathian Braunvieh was established by the cross-breeding of Braunvieh and the small dun mountain breeds of the Carpathian basin. It has been breeding in Hungary about 100 years before and still living in Transcarpathia and Transylvania, but only in very mixed populations. Since it was a characteristic native cattle breed of the country, it should be retain for future generations and re-establish in Hungary. Our primary aim is to preserve the breed’s genetic resources and - if appropriate - to acclaim it as an indigenous breed. If there are no longer residuals of the old type Carpathian Braunvieh, it is still worth to preserve as a local variety, because of its many favourable features. Above all, it’s our duty to maintain the breed, because neither Transcarpathia nor Transylvania has a breeding programme for it.
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Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
105-112Views:289Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
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The Effect of Grazing Intensities on Magnesium Contents
8-13Views:158Research was carried out on two areas of grassland in Hortobágy National Park, Hungary. Two herds of Hungarian Grey Cattle were kept in free range grazing and the effects of grazing pressure on the magnesium content of soil and ryegrass (Lolium perenne L) were determined.
Changes of plant available and total soil magnesium content under different grazing intensities did not show any evident tendency on the investigated grasslands. Different amounts of cattle faeces, urine and trampling had no effect on the magnesium concentration of ryegrass. We conclude that the magnesium content of ryegrass on both grassland sites as moderate grazing and overgrazing matches the requirements of cattle. Symptoms of magnesium deficiency of cattle will likely not appear. -
Macro element contents of different genotype cows’ milk
27-31Views:104The level of mineral elements is important factor regarding the quality of milk. The aim of our research study was to determine the content of mineral elements in milk of Holstein, Jersey, Brown Swiss, Ayrshire, Norwegian-red, Swedish-red cows in the first stage of lactation. All cows were fed with the same type (composition) of feed and they were kept under the same condition. The concentration of macroelements (K, Na, Ca, P, Mg, S) in digested milk samples was determined by inductively coupled plasma optical emission spectrometry (ICP-OES).
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Inner pelvic measurements in dairy breeds
31-35Views:97Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons done on dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jerseys turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshire, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the apparent ideal 1:1 horizontal and vertical diameter ratio; however, this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Although this dairy breed is the lightest, when the pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:108Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:124The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group. -
The examination of presumed Escherichia coli count of raw milk samples on several milk production farms
31-37Views:104For dairy farms, it is of great importance to insure the appropriate hygienic status of milk and to examine it regularly. Escherichia coli, belonging to the coliform bacteria type of, is a good indicator of contamination, and therefore suitable for characterising the hygienic condition of milk production.
The aim of our research was to examine the connection between the Escherichi coli count in bulk tank milk and housing and milking technologies of different-sizes farms. We examined the relation using various statistical methods.
Analysing the connection between the E. coli count and the farm size we found no significant difference between the farms. On the basis of the mean values of the E. coli count, we can say that the hygienic conditions are appropriate for mid-sized farms, and tolerable for large farms. We found differences in the hygienic status among the small farms. Half of the eight small farms, had no adequate hygiene. The results of the analysis of the quality categories show that the probability of inadequate quality milk was the largest on small farms (37.5%).
Comparing the various housing and milking methods with each other, there were numerical differences in the E. coli count, but these differences were not significant. We got higher E. coli count values on those farms using tied stall barn and bucket milking installation. The reason for this could be that in cases of farms using bucket milking installation, it is harder to meet the requirements.
After forming groups by farm size, housing and milking methods, we found that the E. coli counts are adequate on mid-size farms using various housing and milking methods; and tolerable on those large farms using loose housing stable and a milking parlour. At the same time, we found inadequate E. coli counts on the smaller farms using tied stall barns and bucket milking installation.
The results show that if there is suitable attention, independent of farm size, housing and milking procedure, it is possible to produce milk with low E. coli counts, and to insure appropriate hygienic conditions.
Further detailed examinations are needed to decide which factors of housing and milking technologies influence the E. coli count of bulk tank milk. -
Analysis of vitamin E content in pilot dairy products
31-34Views:115Within the framework of Ányos Jedlik program we started an experiment on feeding anti-oxidants with dairy cows at Körös-Maros Biofarm Kft. The purpose of the experiment was to test whether vitamin E and selenium fed as feed-supplement have an effect on the composition of milk produced. Furthermore we examined the cheese and yoghurt made out of the milk to explore how much vitamin E and selenium is retained in the products after processing. Our goal is to develop such functional dairy product which can be part of the daily diet helping us to remain healthy.
After analyzing the data we found out that the anti-oxidant fed to the cows resulted in elevated vitamin E and selenium levels in the milk and that these were retained in the cheese and yoghurt in relatively large amount. -
Effect of vitamin e and selenium supplementation on the antioxidant content of milk and dairy products in dairy cows
9-12Views:113In 2007, the aim of the Ányos Jedlik program and the call for tenders was to support application-oriented, strategic research and development projects, which can increase the competitiveness of the Hungarian economy. In the framework of our project, we intended to examine whether non-protected antioxidants - in this case: vitamin E and selenium – used as feed-additives can increase the antioxidant content of milk. The milk with an increased level of vitamin E and selenium content can be used for producing functional foods which will represent competitive products on the current market of milk products. Our results show that the use of vitamin E and selenium as feedadditives can significantly increase the amount of vitamin E and selenium in the milk and also in the diary products.
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Estimation of Inheritabilities from Holstein-Friesian Test Day Data in Hungary
23-25Views:106Recently, test day models (TDM) began to be increasingly used for the genetic evaluation of dairy cattle. The main advantage of the TDM compared with the 305 days lactation yield models is that more effects can be used in the evaluation. Therefore, the TDM is more accurate than the lactation models. The main disadvantage is the increased computational requirement, but this can be offset by improvements in computer capabilities.
The topic of this paper is the use of a fix regression test day model to estimate the inheritabilities of test day data from Hungarian Holstein-Friesian dairy cattle. The inheritability was 0.26 for milk production, 0.2 for fat production, 0.24 for protein production and 0.06 for the somatic cell count. -
The effect of breed and stage of lactation on the microbiological status of raw milk
37-45Views:296The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.
There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.
Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.
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The effect of season on the microbiological status of raw milk
95-99Views:117Many factors can influence the microbiological quality of raw cow’s milk. In this study, our aim was to determine whether there was any difference between the microbiological statuses of milk produced in different seasons. Samples were collected and analysed from five dairy farms in Hajdú-Bihar County, from February to November in 2019. During our studies, total plate count (TPC), coliform count and Staphylococcus aureus count of raw cow’s milk samples were determined.
There was no significant difference (P>0.05) between the mean TPC values detected in the milk collected in winter and autumn, but that values were significantly (P<0.05) lower than in the milk samples collected in spring and summer. Similarly to the TPC, in the case of coliform bacteria the lowest mean colony count was detected in the samples collected in winter. The difference was significant (P<0.05), compared to the values observed in the samples collected in summer. S. aureus was detected in bulk milk of only two farms in excess of 1.0 log10 cfu/ml. Also in case of S. aureus, there was a significant difference (P<0.05) between the values observed in the samples collected in winter and in summer. Samples from spring and summer contained the highest amount of S. aureus.
Based on the results of our studies, in the case of almost all farms the mean TPC, coliform and S. aureus counts were lower in the samples collected in winter, than in the samples collected in summer. The fact that the samples collected in winter contained the lowest amount of colonies could be attributed to the inhibition of growth of mesophilic microorganisms below 8 °C. Furthermore, the fact that we observed the highest colony counts in samples collected in summer, can be related to the heat stress of cows during the summer due to unfavorable weather conditions (high temperature and humidity).
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Effect of using antioxidants as feed additives in the diet of dairy cows on the vitamin E and lycopene content of milk
69-72Views:99In 2007, the aim of an Ányos Jedlik program and call for tenders was to support application-oriented, strategic research and development projects, which can increase the competitiveness of the Hungarian economy. In the framework of our project, we intended to examine whether non-protected antioxidants – in this case: vitamin E and lycopene – used as feed-additives can increase the antioxidant content of milk. The milk with an
increased level of vitamin E and lycopene content can be used for producing functional foods which will represent competitive products on the current market of milk products. Our results show that the use of vitamin E as feed-additive can significantly increase the amount of vitamin E in the milk. The use of lycopene as feedadditive also gave good results. At the beginning of the experiment, the lycopene content of the milk was below the detection limit, while in the post-feeding milk samples the lycopene became detectable. Based on our results, we are of the opinion that further experiments and analyses are needed regarding the quality of food of animal origin and animal health. -
The role of type traits of dairy cattles in long productive life
11-18Views:92In the last few decades, a new intensive milking stock has developed as a result of breed-converter crossing in Hungary. Additionally, the reproduction biology of the population was adversely affected by keeping and feeding technologies. Productive lifetime has shortened and the service period has lengthened. However, profitable milk production requires cows with longer productive lives and larger life-time productions. In our study, we made a comparison between production, culling causes, and type traits of culled Holstein Friesian cows after first calving and of cows with a minimum of eight lactations. We established that the first lactation production of cows with longer productive lives was more or less homogeneous. Disease of metabolism as well as digestive and respiratory problems, were the main causes of the culling of cows with short productive lives, and reproduction and udder problems as well as low production, were the main causes of the culling of cows with long productive lives. We found a significant difference in the distributions of strength, body depth, dairy form, croup width, rear leg side view, foot angle, fore udder attachment and teat placement between the two groups. Furthermore, we established that cows with lower stature, less strength, a somewhat deeper body, better dairy form, narrower croup width, smaller foot angle, looser fore udder attachment and some outside teat placement had longer productive lives.
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Production and disposition of purebred Jersey populations
89-96Views:98In Hungary crossbreeding wit Jersey has been in practice since 2004. Uniquely in the Hungarian history of the Jersey breed two times fifty head pregnant heifers were imported in 2006. The aim of our study is to present through the description of the production and the type traits of the population the inherent possibilities of Jersey. The breed is presented by the figures of the rolling lactations, 305 day milk production, correlations between the traits and by the results of the evaluation of type traits. Analyses of the data were done by using independent samples t-test.
Based on data of 87 cows the average number of days in milk is 308 and 48 cows has finished lactation. During the rolling lactation they produced 5050 kg milk with 5.34% fat and 3.8% protein content.
The first lactation Jersey cows’ production when corrected to 305 days was 5089 kg milk with 5.35% fat and 3.81% protein. Between the herds there was significant difference in milk kg, amount of fat produced, protein content and amount of protein produced. On the two farms distribution of cows belonging to different production levels is very different. The biggest difference is when categorized by the amount of milk produced.
Correlation between the milk kg and fat kg is the greatest (r=0.950). Similar values were obtained for the correlation between fat and protein kg (r=0.919) and for the milk kg and fat kg (r=0,898).
Disposition of the Jersey cows is very well balanced. Their stature, strength, body depth, dairy form, rump angle and pin width is favorable, the average scores are between 4.5 and 6.5. The leg of the cows is a bit sickly, and is hocking in a little with medium-low angled hoof. Average values of all traits describing the udder are between 4 and 5. Cows belonging to the two herds differ by disposition. Significant difference exists in the following traits: body depth, dairy form, pin width, rear leg - side view, rear leg, rear view, foot angle, fore udder attachment, teat length. The
Jersey population imported to Hungary has a final score of 78 points which equals with the “good” qualification. -
Studies on Ostrich (Struthio Camelus) - Review
15-22Views:906Ostrich has been reared in Hungary for decades, but we have limited information on this species. The aim of this review was to provide a concise description of the ostrich (Struthio camelus) based on international studies. We were to investigate some of the most relevant parameters, such as egg, meat and skin production. In this critical review we managed to sum up the most significant features and productivity parameters of ostrich and effecting factors. So as to make an accurate description of the species we have to know its morphological, behavioural and physiological characteristics. Ostrich is a very special bird with special nutritional and environmental requirements. Ostrich products, regarding egg, meat and skin are considered luxury products not only in Hungary but also abroad. Though egg has a significant nutritional value, it is mainly used for hatching chicks. In Europe we can expect roughly 40–50 eggs from a hen yearly. Ostrich has a lean meat with high protein and low fat and sodium content, moreover it is rich in minerals and polyunsaturated fatty acids. Having these advantageous qualities, ostrich meat should be part of our healthy diet. Skin production is not important in our country, but there is a special demand for high quality ostrich leather globally. In the future our aim is to conduct a research on some of the parameters. As low production rate and embryo mortality is a great deal of problem in ostrich industry, we need to investigate the incubation environment. There is an apparent disagreement among researchers on optimal slaughter age. This is also an area for further investigation. The value of skin and effecting factors on our climate as well as adaptability of the species are also worth for further investigation together with the nutrition what differs from other ostrich breeding countries. Nowadays, there is a great emphasis placed on animal welfare too.
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The possibilities of the development of functional dairy products
33-36Views:119Because of our new aged world’s emerging stress, over exhausting and move less lifestyle the pollution, the not appropriate food consuming and the low calories in the consumed food there are more and more the called „civilian” disorders.
Many people have diabetes and osteoporosis others fight with lung, cardiovascular system, problems and find cancers of many kinds without age exemptions.
There could be a solution in changing lifestyle. In the developed side of the world there are presence of food lines with higher content in nutritious and/or vitamins and fibers such as: kalium, calcium, selen, magnesium, plus it contains less saturated fat. Because of that there is a new word in dietetics as functional foods.
Food having more inner content and/or biological values, so healthier, are called functional foods. Those foods components picked carefully for healthiness by modern knowledge of dietetics.
One of the best raw materials for functional food is the milk. It is already healthy by itself. The Körös-Maros Biofarm Ltd. has a goal of developing and marketing, health protecting organic functional foods in hungaricum products from cured milk. The Jedlik Ányos project helps the Ltd. to achieve this goal. We just finished an antioxidant test and we check if the antioxidant in present in the milk than we check the final product of the presence of the original antioxidant and the form and amount of it.
It had been feeding, for two weeks for three herd of cows selected for age, milk output and consanguinity. All of the three herd had been feeding with basic forage. One of the herds had been getting vitamin-E in the amount of 250 mg/bwkg the other group got licopin in the amount of 200 mg/bwkg once a day each by each orally, the third herd was the control group.
After two weeks we toke a sample of every herd were processed the samples into yogurt and cheese. Than we checked the raw milk, the cheese and the yogurt for antioxidant content with HPLC method. The test ended with good results by finding a great amount of antioxidant, in not only the raw milk but also in the final product.