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Effect of the storage temperature onto the quality of the semen of rams of different breeds
75-79Views:114Semen of 10 Tsigai,3 Prolific Merino and 7 Barbados Blackbelly rams was taken in January, out of the season. Split samples of the diluted semen samples were kept at 23, v.s. 8oC and their motilitywas evaluated daily for 3 days by subjective microscopic investigation. The ratio of motile spermatozoa was strongly decreased during the first day, later the motility rate was sinking more slowly. It could be stated by multivariance analysis that the motility value of diluted semen of Prolific Merino rams was higher and showed a slower reduction as compared to the other two breeds. The decrease of the motility rates was slower at 8oC than at 23oC.
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Factors affecting the efficiency of superovulation and embryo transfer. Season and hormonal background
73-76Views:208Success of the embryo transfer programs is affected by many factors (breed, type, season, treatment). We assume, that periferical blood level of some metabolic hormones (IGF-1, thyroid hormones, leptin, insulin) affects the success of superovulation and ET. According to our results, there is a
significant difference between the in and out of season periferial blood level of IGF-1 and insulin. Furthermore, decrease in the periferic blood levels of IGF-1 and insulin is less drastic in the pregnant recipients. -
Effect of sex on some composition traits of wild boar (wild pig) meat
25-29Views:189The authors examined the nutrition value of the meat of shot wild boars (wild pigs) (n=66) from three wild boar enclosures with different feeding intensity and also the technological properties of the meat. Samples were taken immediately after the evisceration. Considering the storing and processing properties of game meat the samples were taken from m. serratus anterior. As for dry matter examination results, the highest values were measured in case of semi-intensively fed wild boars, then followed the data from the samples of intensively and extensively fed wild boars. The fat content from the meat samples of intensively and extensively fed wild boars proved to be lower while in case of the semi-ntensively fed wild boars it was higher. In females the dry matter content, while in males the fat content was higher. As for the protein content there were no differences in either the feeding groups or in the genders. It was only the water holding capacity of the samples from the meat of the females from semi-intensive feeding intensity wild boar enclosure that fell in between normal values.
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Studying the reproduction-biological effect of the GPR54 receptor gene in sheep
48-51Views:82One of the main economical problems of sheep breeding is that continual production is not possible due to the seasonality of animals. Today, genes, mutations that may develop aseasonality in sheep are extensively researched in order to make the establishment of populations capable of aseasonal estrus possible.
According to the current knowledge, the GPR54 gene participates in the GnRH release from the hypothalamus, and thus in the alteration of the reproduction state of the organism. Our research is aimed to determine whether the GPR54 gene has allele variations that influence the proneness to aseasonal estrus in sheep in a different extent. Therefore, the GPR54 gene sequence of sheep is first examined. -
Examination for Susceptibility of CAE
33-36Views:95Caprine Arthritis Encephalitis (CAE) is a group of diseases in goats caused by a retrovirus, namely the Caprine Arthritis Encephalitis Virus. CAEV belongs to the lentivirus group within the retrovirus family. The CAEV is intimately associated with white blood cells, therfore any body secretions which contain white blood cells are a potential source of virus to other goats in the flock. Once goats are infected with CAEV, it remains infected for life and has to be eliminated.
Since not all goats infected with CAEV progress to disease, very important to test goats for infection using a serology test (ELISA, AGID, IDT). These serological tests demonstrate the presence of antibodies to CAEV in goat serum or detect the virus genome in the white blood cells. However, Swiss results (Dolf and Ruff, 1994) pointed out individual and species differencies in predisposition for this disease. This result was considered as a starting point to our examination. Microsatellite analysis was used in order to find whether there was any association between genotype and serological results, and to look for a marker associated with this disease. To date, altogether 135 goats have been examined. Unfortunately, a significant association between serological results and genotype was not found using the Chi2 test -
Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64Views:147In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.
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Data supporting the quality of sheep milk for processing
67-73Views:82Although the volume of ingredients in ewe’s milk is substantially higher than in cow’s milk, its hygienic quality is lower. The weak quality of raw ewe’s milk limits the possibilities of processing and results in bad quality products. In our investigation we analysed the state of ewe’s milk processing at a typical medium size dairy firm. We investigated the collection, the amount and the quality of milk and the level of ingredients in milk throughout the purchasing period (lactation period).
The purchasing of ewe’s milk was limited to 5 months (from April to September). Although meat (lamb) provides the major source of income to sheep breeders the extension of the period of ewe’s milk production can be beneficial to shepherds and dairy firms. The amount of ewe’s milk ingredients found corresponded to published findings. However, the hygienic quality of ewe’s milk was varied greatly in the different milk samples and these deviations meant bad quality on average. Physiological factors, the circumstances of sheep breeding and milking, the slow cooling of the milk, the little amount of daily milk and the long storage before transportation to the dairy firm together cause poor hygienic quality. The main problem is the long storage time of milk, but our results raise the question of reconsidering the quality classes. Investigating the effect of the hygienic quality of raw milk on product quality, we can get correct data that can be really authoritative. -
Vitrification of mouse embryos by vitroloop technique
81-83Views:86The objective of the study was to vitrify mouse embryos with the cryoloop technology using a new combination of vitrification mediums. Embryos were exposed to a 2- step loading of CPA, ethylene glycol and propylene glycol, before being placed on the surface of a thin filmy layer formed from the vitrification solution in a small nylon loop. After warming, the CPA was diluted out from the embryos by a 3-step procedure. Our data show that a high percentage of embryos survived (92.7%) vitrification in the mixture of EG and PG combined with cryoloop carrier and developing normally (89.1%) in vitro after thawing.
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Lambing Frequency in the Ile de France, Suffolk and Babolna TETRA Stocks of Babolna c.o., Szendrő
29-32Views:115Lambing frequency of three different genotypes was evaluated in the breeding stock of Babolna c.o. Szendrő. Ile de France, Suffolk and Babolna TETRA breeding ewes gave the data for the further evaluation. The main aim of this research was to find out if there is any significant effect of selected parameters (registration class, lambing season, lambing type and the years) on the lambing frequency, the period between consecutive lambings. Production parameters of 9,471 Ile de France, 1,244 Suffolk and 7,618 Babolna TETRA ewes were collected and processed. The analysis covered the period between the years 1989-1999. It was concluded that not all of the evaluated parameters had a significant effect on lambing frequency. Not all of the genotypes showed significant differences, according to the examined parameters.
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Carcass and the Meat Quality of Hungarian Lambs
65-71Views:93The author investigated 153 lambs of nine genotypes originating from breeding flocks, and 50 lambs originating from production flocks. The investigations were performed between 1995 and 1998. The authors discuss the evaluation of comformation and fat cover according to EUROP standards. The authors also investigate the proportion of valuable meat by genotype, and – out of the internal value indicators – the dry-matter, protein, fat, connective-tissue and hemin contents comparing the flavour, aroma, tenderness, and oven loss of the different genotypes.
The following findings should be highlighted:
♦ The Hungarian Merino breed should be improved, as – according to EUROP standards, more than 70% of these animals were rated as quality „R”.
♦ Hungarian fattening technology has to be preserved, as the lambs reach the desired slaughter weight within a short period of time, and without over-fattening.
♦ Readiness for slaughter, typical of each genotype, has to be defined, and slaughter at proper weight be achieved.
♦ It has to be re-evaluated whether the Hungarian Merino is the only breed which can be used in Hungary, as none of the investigations really proved the special characteristics and significance of this breed.
The author summarizes the findings of the investigation in five tables. -
Effect of selenium supplement on proteome of chicken liver
9-13Views:137The aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.
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Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:124The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group. -
Identification of cow’s and buffalo’s milk and dairy product using a DNA-based method
279-282Views:138Aim of our study was the optimization of a DNA method, that is appropriate for reliable, low cost identification of animal species in milk and dairy product (cheese) and to determine the ratio of species. Mitochondrial DNA was used in our work to analyse buffalo/cow milk mixtures contained different ratio of bovine milk such as 0.1%, 0.5%, 1%, 1.5%, 2%, 5%, 10%, 15% (v/v%). Buffalo cheese were produced using buffalo and cows milk (0%, 2%, 5%, 10%, 15% – v/v% cows milk in buffalo milk). In case of milk mixtures, using species specific primers, the PCR assay showed a 0.5 v/v% detection limit. Cattle, in the buffalo/cows milk 99.9/0.1 v/v% mixture, was not detectable. The identification of buffalo and cows DNA in cheese was successful. The intensity of eletroforetic PCR fragment indicated the increase of cow milk ratio in milk and cheese samples as well.
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The analysis of the milk production and the body condition on two Saanen goat-farms
53-59Views:79The authors carried out an examination on two Saanen goat farms during four months from May to August 2008. The body condition score and milk production of the goats were measured. In data processing analysis of variance was used to compare the data of the studied farms. They tried to find the answer for the question how the body condition scores and milk production change in each month. The changes observed in each farm were examined separately then they compared the values of the two farms.. On one of the farms the nutrition was proper therefore with an ideal, intermediate body condition (average 2.73) the goats produced a good amount of milk (2.5 milk-kg/mother/day). On the other farm the nutrition was not proper, therefore the body condition of the goats was weak (average 1.88). Their milk production decreased significantly from month to month (average 2.35 milk-kg/mother/day). The authors could conclude considering both farms that the more milk the goats produced, the lower their body condition score became. The two parameters showed negative correlation.