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Damages caused by winter frosts, their temporal variation and frequencies in the main fruit growing region of Transdanubia and of the East Tisza regions of Hungary
Published July 26, 2012

The aim of the study was the study of winter frost damages, especially their changes expressed in temporal frequencies on the main fruit growing regions of the country. In our earlier paper, we introduced the calculation with the term LT50 as the quantitative expression of temperature threshold, when the lethality halves the survival of plant o...rgans, buds or cells causing 50% death rate. The damage is highly dependent on the temperature and on its duration (length of time), but not at least on the frost tolerance of the fruit trees. The incidence and severity of damage is analysed according to the apricot and peach varieties of their different susceptibility or tolerance too. Four fruit growing regions, two of the in Transdanubia and two belonging to the regions east of the Tisza river have been selected to trace the incidence and severity of frost damages. For that purpose, we analysed the history of the past 60 year period, 1951–2010, utilising the database of the network of 16 meteorological stations of the countrywide service. Being aware of the values of LT50 during the rest period and afterward, the compulsory dormancy caused by low temperature, the number of days, the probability of frost damage could be predicted. The role of the orographical profi le, the height above sea level and the exposition of plantations are also decisive. Within the same plantation, 20–30 m difference of level may cause large diversity in temperature and frost damage. Air circulation and regular incidence of winds within the Carpathian basin modify the occurrence and severity of damages. Lowlands near the southern and northern country borders are particularly exposed to winter frosts. Most damages are reported in February, as temperatures below –20 °C especially if the fi rst part of the winter was mild, or in January was a warm period. With the end of the physiological rest period of the trees, the frost-susceptibility increases signifi cantly, and a cold period of –15 °C may cause heavy damage. This study proves that tolerance of varieties infl uence the damages substantially. By planting frost tolerant varieties, winter frost damages could be diminished by 40–50% at the same growing sites. Present results may also offer a tool to estimate the risk of frost damages and express the security of yields at a given site based on the data accumulated in the database over many years.

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Microsporogenesis of peach (Prunus persica L. Batsch) varieties
Published October 16, 2002

Bud dormancy during winter is a critical factor in peach production in Hungary. The yield is determined basically by the survival rate of flower buds during winter frosts and by their ability to develop normal floral organs. It is important to investigate the genetic basis of slow floral development during dormancy for the purpose of breeding p...each varieties with better winter hardiness. The aim of the present research was to examine microsporogenesis in 14 peach varieties during three successive winters in a Hungarian germplasm collection and to study the effectiveness of this method in variety evaluation. There were significant differences in the dynamics of microsporogenesis both between the varieties and between the years. Of the varieties, ‘Mayfire', bred in California, possessed the quickest pollen development rate. The microsporogenesis of `Piroska', a Hungarian local variety, was the slowest. Rapid floral bud development was observed in `Aranycsillag', `Springcrest' and 'Venus'. A medium developmental rate was characteristic of `Babygold 6', Fairlane', `Michelini' and `Red June', while development was slow in 'Champion', 'Early Redhaven', `Redhaven', `Harko' and `Mariska'. Based on these results, the study of microsporogenesis represents a reliable method for the phenological description of peach varieties during dormancy. The application of this method makes it possible to identify varieties and landraces with slow flower bud development, suggesting better winter hardiness.

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In vitro comparative study of two Arundo donax L. ecotypes’ selenium tolerance
Published September 7, 2014

Selenium tolerance of two somatic embryo-derived Arundo donax L. ecotypes (Blossom, 20SZ) were compared in in vitro culture. Sodium-selenate (1 – 100 mg L-1) as known the most phytoavailable selenium form and the less studied red elemental nanoselenium (100 mg L-1) were applied as selenium treatments. Basis on the results Blossom ecotype seem...ed to be more sensitive to the sodium-selenate than 20SZ. Inhibiting effect of selenate was effectuated above 10 mg L-1 in case of Blossom, which was manifested in decreased survival rate and growing parameters. Contrast to this 20SZ could tolerate the selenate ≤ 20 mg L-1 without any toxic symptoms. Lower selenate tolerance of Blossom could be explained with higher selenium accumulation. Both of two ecotypes could also uptake and accumulate the red elemental nanoselenium however in much less extent compared to selenate.

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Micropropagation of an orchid Dendrobium strongylanthum Rchb.f.
Published March 19, 2007

A simple and reliable procedure for in vitro propagation of an orchid Dendrobium strongylanthum Rchb.f. was studied. Protochorm was induced from seed explants on 1/2 MS medium supplemented with 0.2 mg/L NAA. A mass of protochorm could be multiplied on proliferated medium of 1/2 Ms containing 0.5 mg/L V-6-BA. And bud differenti...ation of green global body was cultured in the same media, 2-2.5 cm shoots were formed after 30 day of culture. Addition of mashed banana and 0.5 mg/L NAA to 1/2 MS medium promoted root formation and vigorous growth. The plantlets were acclimatized and transplanted to compound materials of humus:sawdust (1:1) in greenhouse, the survival rate was more than 98%.

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Effects of activated charcoal on rooting of in vitro apple (Malus domestics Borkh.) shoots
Published March 21, 2001

Rooting of in vitro 'Royal Gala' shoots was studied under different conditions of root induction and root elongation phase. The rooting capacity was affected by both rooting phases. Very high rooting percentage could be reached with both liquid and solid root induction media. Raising the temperature from 22 °C to 26 °C during root in...duction phase increased the rooting percentage. Presence of activated charcoal in root elongation media can affect the number of roots per rooted shoots and can increase the rooting percentage, the length of roots and the rate of survival depending also on other conditions during rooting. Presence of NAA in root elongation media reduced the number and the length of roots considerably. Favourable effect of activated charcoal on rooting was mainly due to adsorption of NAA.

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Comparison of flower bud development in almond, apricot and peach genotypes
Published April 19, 2006

The phenological processes of flower bud development of stone fruits during dormancy are not thoroughly known. The yield of these species, especially of almond, apricot and peach is determined basically by dormancy of flower buds, the survival rate of buds during winter frosts and by their ability to develop normal floral organs in the next After the initiation of floral primordia, flower bud development is taking place in continuous space until blooming, though at different speed characteristic to the species. To study flower bud development during dormancy we applied two alternative methods in different genotypes of almond, apricot and peach: (1) examination of pollen development (microsporogenesis), and (2) the measurement of pistil length. The samples were collected from the central part of Hungary during the dormancy period of 2004/2005. The three fruit species differed significantly in the speed of flower bud development, it was the quickest in almond, followed by apricot and peach. In addition to the species, there were significant differences in the process of microsporogenesis and pistil development between genotypes within species and also between the different types of shoots on which the buds were located. On short shoots buds developed at a higher speed, than on long shoots. Based on our observations, on the short shoots the period of endodormancy was shorter with 5-30 days, according to genotypes, compared to the long shoots. This difference, however, decreased to 2-3 days by the time of blooming.

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New in vitro micrografting method for apple by sticking
Published August 23, 2000

The requirements for in vitro micrografting in apple are described. In vitro multiplicated shoots of cv. Royal Gala were the sources of rootstocks and scions after different pre-treatment, respectively. Oxidative browning of cut surfaces could be inhibited by the use of antioxidant mixture during grafting process. Sci...on base cut in v-shape was stuck by 1% agar-agar solution into the vertical slit of rootstock. There was no any displacement and the rate of fused and further developed grafts was 95 percent. Agar-agar between the rootstock and scion made the transport of different materials possible and hold the graft units together until the fusion took place. Fusion was proved also by histological studies. Some of in vitro micrografts were planted and acclimatisated and the survival was 100 percent.

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