Investigation of flowering dynamics of the basil (Ocimum basilicum L.) and its consequences in production

The flowering dynamics of Ocimum basilicum L., (a common population maintained at the UHFI) was studied with the aim to create an exact and practically applicable method for definition of phenological phases.

According to our observation the development of individual flowers of the basil can be characterized by 8 distinguishable phases, which must be considered for description of the actual phenological stage of a spike.

An accurate model was created for the unambiguous description of flowering process of different flowers within the spike and for the individual plant as a whole. The new flowering index formula is calculated from the number of flowers weighed by their phenological phases.

The time dependence of flowering is presented by functions fitted to values of the flowering index. The results reflected different patterns of the main inflorescence, the inflorescences formed on the side shoots in the first or in the second half of the flowering period. However, for description of the flowering process of the whole plant, a sigmoid function proved to be the appropriate model.

The accumulation process of the essential oil could be characterized by the flowering index values. They showed close correlation (r=0.964) at high probability.

The new method assures an exact definition of phenological phases in basil. Its application seems to be optimal during breeding procedures, seed production and even in production of high quality drugs, according to the requirements of the Good Agricultural Practice (Guidelines for GAP, 1998).


Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)

The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.

Effects of silicon in plants with particular reference to horticultural crops - Review article

Silicon (Si) has long been considered as non-essential element for plant’s growth and production. Numerous efforts are being made for the discovery of its beneficial effects with large scale studies laying foundation for new findings and hypotheses. Therefore, Si has been suggested to be a quasi-essential element due to its positive effects against biotic and abiotic stresses alike. Though Si is the second most abundant element in the soil profile, its availability to plants is limited to the form of monosilicic acid only. Besides, plants’ ability to take-up Si and use it in their physiological processes also depends on the available transporters associated with it. Thus, the present review covers uptake and transport of silicon in plants as well as Si mediated physiological processes, including mechanisms underlying induced tolerance against biotic and abiotic stresses with a particular emphasis on horticultural species.

Scheduling of ornamental plant production

The production of ornamental plants represents an important branch of our horticulture. The growing area is relatively small (round 2800 ha), the production value, however, a rather large. Notwithstanding, its financial balance is inactive because the demand surpasses the supply copiously.

The most popular plants are as follows: carnation, gerbera, rose, bulbous flowers, chrysanthemum, other cutflowers, cutgreens and Gypsophyla — their total production value makes up to Ft 8-9 billion. The area of ornamental nurseries — about 800-900 ha — is to be found mostly on the western part of our country.

Our accession to the EU will have undoubtedly an impact on our ornamental plant production. We must take into account, that in greenhouse production the specialization extends all over the world, field production over a limited region. Our chances will not deteriorate by joining the EU. The buyer — chiefly because of ecological purposes — will prefer the domestic product to the foreign one. By the way, our products will be competitive, as far as quality or price is concerned, with those of western Europe. Last but not least, we may hope a greater saleability of home-bred, special varieties and cultivars, the so-called "hungaricums", both in the domestic as well as export markets. It is anticipated that we will have a good turnover with potted ornamental plants and flower seedlings. Our nursery products will become marketable too.

To exploit the opportunity, of course, the necessary conditions are to be created. First of all, we need development in research, with special regard to breeding, at the same time in education, in extension service, in the training of experts, on a high level. Some tasks can be solved, no doubt by improvement of the organisation within the branch. At the same time the state subsidy is indispensable in order to promote both the technical and the research activities. State subsidy is also necessary to build up more advantageous conditions of sales like in some foreign countries (e.g. the Netherlands).

Production and marketing of medicinal and aromatic plants

The utilisation of medicinal and aromatic plants is growing continuously, which is motivated by both psychological and rational factors. Species belonging to this special group of plants were produced in the territory of Hungary many centuries back. However, the intensification of the production started in the first years of 20 th century, only, and in spite of many political and economical contradictions the medicinal and aromatic plant sector became a successful part of Hungarian Agriculture. Some of the national products had been accepted as a special Hungarian product ("Hungaricum") evaluated as most respectful in the world market. To stabilise, even to develop the production of medicinal and aromatic plants in Hungary the understanding of the actual world situation, especially the forthcoming EU conditions, became essential. Based on the analysis of the situation market and economy of medicinal plant production, a new strategy had to be developed. The medicinal and aromatic plant sector requires a special development program, which should be supported by Hungarian authorities, focusing on the subjects, which are as follows:

  • development of "quasi" EU compatible Hungarian regions specialised for medicinal plant production,
  • development of biological background, technology as well as the quality assurance system of the production,
  • EU compatible regulation and subvention system of the sector, including processing, production and marketing of raw and phytomed ici nes,
  • development of new marketing strategy in both national and international relations.


Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA

Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-life has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.

The effects of ACS (1-aminocyclopropane-l-carboxylate synthase) gene down regulation on ethylene production and fruit softening in transgenic apple

A detailed examination of the production of ethylene and other ripening parameters during storage period has been undertaken in transgenic apple fruits, where the ethylene biosynthesis was inhibited by antisense ACS (l-aminocyclopropane-l-carboxylate synthase) gene. Data indicate down regulation of ethylene production, softening and spoilage in some transgenic lines. In some cases ethylene production was inhibited for over 90 percent, considerable reduction of softening and spoilage was observed probably due to the reduced activity of cell wall degradable enzymes. ACS activity was also monitored during ripening. The fruits of the best transgenic lines could be stored for minimum 4-5 months longer under 5 °C cold room storage conditions and one month longer at normal room temperature. This molecular approach can provide an alternative way to replace the commonly used and costly atmospheric storage of fruits.

Production of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) gene

Dianthus chinensis and Dianthus caryophyllus varieties were tested for shoot regeneration from leaf and petal explants and transformed with Agrobacterium tuniefaciens strains (EHA 105 and LBA 4404) harbouring an apple derived ACS cDNA in antisense orientation in order to reduce ethylene production and influence the ethylene dependant traits in carnation. After transformation regenerating shoots were selected on MS medium containing 50-75-100-125-150 mg/1 kanamycin and supplemented with 1 mg/1 BA, 0.2 mg/1 NAA. Transgene integration was proved by PCR analysis with npt II spcific primers followed by Southern hybridisation of DNA isolated from green shoots on medium containing 150 mg/1 kanamycin. Several putative transformants were subjected to RT-PCR in order to examine the npt 11 expression at mRNA level. Both the transformant and the non-transformant plants were potted into glasshouse to observe the effect of changed ethylene production on flowering time, petal senescence and vase life.


Evaluation of Colour Versions of Wild Sage (Salvia nemorosa L.)

In the continental weather zone, more and more frequently occurring extreme conditions require continuous renewal of the market which generates constant challenge for the ornamental plant breeders. Most of the traditionally used decorative ornamental plants are sensitive to these extreme conditions. In 2001, Department of Plant Biotechnology, Debrecen University initiated an interdisciplinary breeding program in collaborations with Zoltan Kovats (he dealt with hungarian drought-tolerant plant species) to produce new or reintroduce forgotten drought-tolerant ornamental species into public parks and roadsides. From ~900 species of Salvia genus, Salvia nemorosa L. has been known as a medical plant, however, because of its high adaptation ability and decorative nature it is a highly recommended ornamental plant as well. Salvia nemorosa L. has a low maintenance, extremely droughttolerant, fast growing plant, generates proper cover, and highly competing weeds on roadsides. Nowadays, 50-60 varieties are available; however this number could be increased by new hybrids. Great morphological and colour variation could be seen within the species, from different white to deep violet. The main goal of this research is the production of elite lines with wide colour and morphological variation in wild sage. We have already obtained 25 different clones for further investigation without eliminating the original plants generating an in vitro gene bank as it has been done by Italian breeders.

Analyses of the pathogen and weather components of disease progress for modeling apple scab epidemics in integrated and organic production systems

The pathogen and weather components of apple scab disease progress were analysed in a three-year study, in two environmental-friendly production systems (organic and integrated) on cvs. `Idared', `Jonica' and 'Mutsu'. Linear regression analyses of transformed disease incidence and severity data and "area under the disease progress curves" (AUDPC) were used for the analysis of the pathogen component. To evaluate the role of the weather component in apple scab epidemic, first, the weekly disease increase was determined at a certain week (n). Weekly disease increase was related to rainfall, relative humidity, Mills' wetness period, temperature and interaction between temperature and relative humidity. Five different periods were used in the analyses: i) week (n-1), ii) week n(n-1), iii) week (n-2), iv) week (n-1)(n-2) and v) week n(n-1)(n-2). In the analyses of the pathogen component, the best transformation function was the logistic one. Regression analyses showed that disease growth rates were higher for disease incidence and for the organic production system than for disease severity and for the integrated production system, respectively. Disease growth rates for leaf incidence were higher than fruit incidence on all the three cultivars. AUDPC values showed great differences in both leaf and fruit incidences among cultivars and between the two production systems. The results the of analyses of the weather component showed that the best relationships between disease increase and weather parameters were found for fruit incidence and leaf incidence in week (n-2) in the organic and integrated production systems, respectively. Results also demonstrated that in week n(n-1) temperature played a more important role in the fungus development than the water parameters (relative humidity, rainfall and leaf wetness). Consequently, infection process is significantly dependent on almost all weather parameters, but during the incubation period the most important weather parameter is the temperature. Results were compared with similar studies and biological interpretations of the analyses are discussed.

Economics of sea buckthorn production and processing in Hungary

This study focuses on the business management-related advantages and disadvantages of sea buckthorn production and processing based on economic analyses. It is the main objective of the authors to identify the expected economic findings in a high standard plantation with different average yields. A deterministic model calculation was performed on the basis of technological processes, using the primary data collected from enterprises dealing with sea buckthorn production. The calculation is based on the assumption of a 10 hectare plantation with intensive production technology (high soil quality (golden crown value: 32 GC per ha), irrigation, high plant density per hectare). The cost and income relations and the long-term return of the plantation were examined in the case of different average yields (12 t ha-1, 18 t ha-1 and 24 t ha-1). Under the economic circumstances of 2016, the planting cost of an intensive plantation is around 4-4.1 million HUF ha-1. In the years following the fruit-bearing stage, direct production costs are between 2.5-3.9 million HUF ha-1, depending on the given average yield. On the contrary, 5.6-11.1 million HUF ha-1 revenue can be reached based on the current market prices, resulting in a gross margin of 3.1-7.1 million HUF ha-1. Under the modelled circumstances, return is realised on the plantation’s costs in 6-8 years. The net present value (NPVr=3.24%) calculated for the 15-year-long life cycle of the 10-hectare plantation is between 151-466 million HUF, while the internal rate of return (IRR) is between 23-45%. From the business management aspect, the advantage of sea buckthorn production is that it provides better income and return at a planting cost which is similar to that of other small fruits and berries. At the same time, the disadvantage of sea buckthorn production is the fact that yields are harvested every two years due to the technological characteristics of harvesting. The negative impact of this bi-yearly yield on liquidity can be eliminated with the so-called delayed planting.

Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.

The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.


The impacts of different habitats on the development of Telekia speiosa (Schreb.) Baumg.

Telekia speiosa (Schreb.) Baumg. is a 100-150 cm high bushy perennial, which has yellow flowers and smells good. According to the descriptions (Farkas, 1999), it can be detected in two smaller areas within Hungary, namely in the Bükk hills and on the Szatmár-Bereg Plain. By the time of writing this paper, the population around Tiszabecs has already got extinct. Therefore, it is a protected relict species. It is named in honour of Sámuel Teleki, chancellor of Transylvania.Within the frame of the experiment, the Telekia speciosa (Schreb.) Baumg. was planted to places differently illuminated (sunny, semi shadow, shade), then the morphological changes brought about the various light conditions were investigated. The experiment was launched with a stock sown in October 2008. The seedlings were planted to three beds with diverse light conditions. The area of each bed was 1 m2, and ten seedlings were planted per m2. The parameters investigated are as follows: the length of leaf blade, the width of leaf blade, the length of petiole, the number of leaves per plant, and the alterations of leaves. As a result of our research, we can state that semi shadow is the optimal habitat for the plant. Under such ecological conditions the highest leaf production was observed, the leaves were species specific, healthy and big. The mean number of leaves per plant was 6.6, the mean length of blade was 16.6 cm, the mean width of blade was 13 cm, while the mean length of petiole was 14.2 cm. In the shade the plants grew poorly and the size of leaves were smaller. The mean number of leaves per plant was 4.1, the mean length of blade was 8.6 cm, the mean width of blade was 7.1 cm, and the mean length of petiole was 9.4 cm. In the sunny habitat a similarly high leaf production was observed as in the semi shadow; however, the leaves had brownish spots, they shriveled, feel rough, so they revealed a reduced aesthetical value. The mean number of leaves per plant was 6.6, the mean length of blade was 17.8 cm, the mean width of blade was 11.3 cm, and the mean length of petiole was 13.1 cm.

Investigation of some production factors on yield and quality of dry beans

The correlation between plant density and yield average shows that the volume of yield increases in varieties of large and medium-size seeds up to a plant density of 285-400 thousand/ha, after which it declines.

On the basis of the results, yield averages at plant densities of 285-334 thousand plant/ha are 0.17 t/ha higher than those achieved at low density (200 thousand plant/ha).

High levels of potassium fertilizer did not enhance the yield of dry beans. With adequate-water supply the high level of potassium decreased the number of pods and seeds per plant compared with a basic level of fertilizer, which could ensure 2.5 t/ha yield.

Giant reed (Arundo donax L.) from ornamental plant to dedicated bioenergy species: review of economic prospects of biomass production and utilization

Giant reed (Arundo donax L.) is a perennial, herbaceous grass, it has been spread all over the world from continent to tropical conditions by human activities. In continental climate, especially Hungary, it has been considered as ornamental species, due to its decorative appearance, striped variants’ colour of leaves, long growing season and low maintenance requirements. It does not produced viable seeds, so it can be propagated vegetative ways by rhizomes or stem cuttings and by in vitro biotechnology methods. Because of its growth habits and good adaptation capability, it has been considered invasive weed primarily in coastal regions in warmer climate areas. In the previous century, giant reed produced for paper/cellulose/viscose production, woodwind musical instruments, stakes for plants or fishing rods etc. Over the last few decades, it has been produced for bioenergy purposes (bioethanol, biogas, direct combustion) or utilize as chemical basic compounds or construction materials. It has been considered a dedicated promising biomass crops thanks to high biomass production, high energy balance of cultivation and adaptability of different kind of soils and conditions. The objective of the present paper is to overview the most significance literature data on giant reed production and utilization, compare to own experimental data and economic calculations and to determine some critical factors, advantages and disadvantages of giant reed production compare to other biomass species.

Rate of scab infection and quality parameters of apple fruit in organic and integrated production systems

In this study, the rate of scab infection and quality parameters (content of vitamin-C, dry matter, sugar, total acids, specific weight, quality index and flesh firmness) of some apple cultivars are given, moreover, the relation between those characters was examined in two environmental systems (organic and integrated) in a rainy season. In the organic fruit production, scab infection of leaves was significantly higher than in the integrated system, in case of the same cultivars, which means that the rate of decrease in leaf area was heavier in the organic production. Cultivars did not differ statistically in parameters of inner content when the integrated system was compared to the organic one. Results did not show statistical difference between loss of leaf area caused by apple scab and the parameters of inner content, which is attributed to the regeneration of leaf area due to favourable weather conditions. Data on apple scab and inner content of the fruit justify essentially the environmental systems of apple production.


Effect of pruning on disease incidence of apple scab and powdery mildew in integrated and organic apple production

In a three-year study, the effect of two winter pruning treatments (strong and weak) on apple scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) was evaluated on six apple cultivars (`Rewena', `Elstar', 'Liberty', 'Gala', 'Pilot' and `Jonica') in two environmentally-benign apple production systems (integrated and organic). Results on apple scab showed that compared to the integrated production system, the pathogen caused significantly more infection on the leaves of cvs. `Elstar', 'Gala' and `Jonica' in the organic production system. The effect of pruning treatments on apple scab was mainly detectable on the cultivars susceptible to the disease such as cvs. 'Gala' and `Jonica'. The weak pruning correlated with a higher level of apple scab infection than the strong pruning, especially in the organic production system. Primary mildew infection was very low in both protection systems (below 2 %), therefore, effect of pruning on primary mildew infection could not be analysed. Low secondary mildew incidence was detected in the organically treated orchard part; however, incidence of powdery mildew was significantly higher (P> 0.05) on cvs. 'Elstar', 'Pilot' and `Jonica' in the integrated orchard part compared to cvs. 'Gala', 'Rewena' and 'Liberty'. The highest incidence of mildew was on cv. 'Elstar'. Pruning had very low effect on incidence of secondary mildew infection on leaves.

Perspectives and tasks in horticultural production

The work summarizes the prospective conceptions of all the five horticultural branches. These branches (vegetable, fruit, grape and wine, herb and ornamental plant production) with the production of propagating material together amount to round 30-35% of the total value of the entire plant production. The performance of horticultural branches declined significantly because of privatisation and lack of capital. The accession to the EU urges the development of modernization and competitiveness, therefore the state subsidies are indispensable.

Impact of organic and inorganic fertilizers on the growth and yield of cabbage in Ghana

A field experiment was conducted at Dormaa Ahenkro, Ghana, to determine the impact of inorganic and organic fertilizers on the growth and yield of cabbage. The experiment was laid in a randomized complete block design with three replications. The experiment treatment comprises of sole application of poultry manure (PM), NPK 15:15:5, the combined application of poultry manure and NPK fertilizer (PM+NPK) and the control (no fertilizer). Data was collected on the number of leaves, stem girth, plant height, head diameter, head weight and edible head weight. There was a significant difference (P<0.05) among all the treatments. The combined application of PM+NPK recorded the highest values for all the parameters measured. From the results obtained farmers should consider the combined application of PM and NPK to maximize yield on their farm.

Improved evaluation method for reliable distinction of poppy (Papaver somniferum L.) cultivars

Investigating seven Hungarian standard cultivars (i.e. 'A1’, 'Kék Gemona', '13C-2`,'Kék Duna', `Kompolti M’, `Gödi N',

'Kozmosz) it was proved that for the reliable distinction between cultivars a complex analysis is necessitated. Because of the large morpho­phenological and production-biological plasticity of the species qualitative and quantitative features as well as alkaloid accumulation characters have to be evaluated simultaneously applying discriminant analysis.

It was proved by the investigations that some of the qualitative and quantitative characters included into the DUS evaluation procedure have slight importance for reliable distinction. As an example the majority of cultivars show high inhomogentity in such characters like the shape of stigmatic disc, the number of stigmatic lobes etc. Furthermore these characters never had been chosen for the goal of any practical selection and are modified by the weather conditions as well.

In the evaluation process the alkaloid accumulation characteristics (both qualitative and quantitative ones) have to be evaluated on a higher rank. For instance the high accumulation level of morphine in cv. 'A1' (10.5 %o), exceptionally high narcotine accumulation in 'Kék Gemona' (7.8 %o), or the low morphine accumulation level in cv. 'Kozmosz' (1.1 %o) proved to be proper characters for their distinction, alone. However, at the same time the weather conditions existing at the time of experiment have to be taken into consideration. From practical point of view, the years rich in precipitation and poor in sunshine are less suitable for distinction of cultivars selected for higher alkaloid accumulation, especially for production of codeine and thebaine characterized by a higher methylation level. Based on the example of 'Kék Gemona', the accumulation level of morphine decreases from 7.4 %o to 4.1 %o under such conditions, while thebaine and codeine disappear, practically.


Foliar Nutrition and Post-Harvest of Onion Seed: Effects of storage temperatures, storage period and foliar nutrition

The aim of onion bulb storage is to meet consumer demand for extended availability of onions whilst maintaining product quality. The principal biological factors leading to onion bulb deterioration are respiration, resumption of growth and pathogen attack. In onion bulbs a dormant period, when sprouting and rooting cannot be induced, is followed by a period of internal changes that prepare the bulb for breaking of dormancy and subsequent growth. Out of storage, the bulb then proceeds towards flowering and seed production. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on yield and yield components of onion seeds and storage The seed yield of each commercial compounds plot from previous experiment was divided into two groups, storage under room temperature and 5°C. Seeds transferred immediately after drying to Increasing Export Competition of Some Vegetable Crops Project Laboratory located in Faculty of Agriculture, Cairo University. The effects of storage temperature, storage period and foliar with some commercial compounds on onion seed quality were considered. Storage in 5°C had the higher germination percentage than storage in room temperature. Results indicated that as storage period increased the germination percent decreased. The treatment with boron or amica in the first season had the highest germination percentage. While, the treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer or amino X had the highest germination percentage in the second season. Storage in 5°C resulted in higher moisture content than storage in room temperature. Regarding the effect of storage period on moisture content, the water content was significantly increased with prolongation of storage period. The lowest values of water content were recorded for treatments with union feer, shams K or boron in the first season, and union feer, shams K, boron, magnesium, shetocare or hummer in the second one. Catalase activity was significantly decreased as storage period increased. The treatment with shams K, boron, shetocare or amino X had the highest catalase activity in both seasons. Peroxidase activity was significantly decreased as storage period increased. Foliar application with boron had the highest peroxidase activity in both seasons. Seed stored in room temperature had the higher malondialdehyde content than those stored in 5°C in the second season. The malondialdehyde content increased as storage period increased. The treatment with magnesium, caboron and the control in the first season, and the treatment with magnesium and the control in the second season had the highest malondialdehyde content.

Dr. Ottó Orsós, the forgotten Hungarian pioneer in plant tissue culture

The knowledge of tissue culture deserves attention in respect of understanding the development of universal biology. This study intends to contribute to the past of the plant tissue culture by such data of the history of science which have been unprocessed so far. It seems that the life-work of the Hungarian biologist, Dr. Ottó Orsós is a missing and essential link between those early plant hormone researchers and the representatives of the pioneers of tissue culture schools who have contributed substantially to the development of the modern in vitro plant morphogenesis and plant cell biology. Orsós cultured kohlrabi tuber cubes on White culture medium in a sterile manner. This way, he could efficiently direct the in vitro morphogenesis of the kohlrabi, the regeneration of its shoot and root, and the formation and steps to subculture of pure callus tissues in 1938. He supported the correctness of its statements by means of detailed anatomical examinations. Orsós successfully rooted and aclimatized complete regenerated plants. We may as well call the above system — in remembrance of the creators of the original concept — "Haberlandt-Orsós model". Between the publishing of his main paper in 1938 and 2003, a period of 65 years has lapsed. On the occasion of this anniversary, we bow before this forgotten pioneer.

Bioactive phenols in leaves of Forsythia species

Nowadays a number of lignans (arctigenin, matairesinol, pinoresinol and phillygenin) have come to the fore in research due to their various biological activities. In this paper the accumulation of these constituents in leaf extracts of Forsythia plants (F. intermedia, F. ovata 'Robusta’ and `Tetragold', F. suspensa, F. viridissima) was quantified using a new isolation method, supercritical CO2 fluid extraction. The total phenolic and flavonoid contents, the antioxidant capacity and the aglycone lignan profile were determined in leaf extracts of Forsythia species. Within the phenols, the flavonoids were only present in small quantities, but the amount of aglycone lignans was extremely high. F. ovata `Robusta' had the highest total lignan content (103.8 mg/g) of all the Forsythia species. The main lignan in this species is arctigenin, which normally makes up about 60% of the total lignan content, but in the case of F. ovata `Robusta' this value was 96.1%. Since this arctigenin content is outstanding compared to that of other Forsythia species, it could be promising to develop a fermentation technology for the production of this natural compound.

Effect of Foliar Nutrition on Post-Harvest of Onion Seed under Sandy Soil and Saline Irrigation Water Conditions

Foliar application has been determined to be an effective nutrients delivery strategy in vegetable and fruits. The enhancement of vegetable and fruit yields affected by foliar nutrients application has been recognized in previously conducted studies with perennial tree crops. The efficiency of foliar nutrition is dependent on soil, climate, fertilizer and the amount of nitrogen used. There is no sufficient information concerning cooperation of foliar nutrition with all nutrients form as well as the rates of these nutrients fertilization in vegetable
and fruit crops. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on inflorescences diameter, flower stalk length, number of seed stem /plant, weight of 1000 seed, germination percentage, seed yield, moisture content, catalase , peroxidase activity and malondialdehyde content of onion seeds. The plants
sprayed with union Zn, union Mn, union feer, shams k, elga 600, boron, and amino x had the highest vegetative growth parameter, germination percent and enzyme activity. The plants sprayed with union Zn, union feer, shams K, magnesium, caboron, hummer and amino X had the highest seed yield ha-1. The seeds were stored for one year to study the effect of different commercial compounds and storage temperatures on germination, moisture content and change in antioxidant enzymes activities of onion seeds during the storage period. Storage at cold temperature showed higher germination percent, moisture content and lower malondialdehyde content than storage at room temperature. The treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer and amino x had the highest germination percent.

Fire blight in Hungary between 1996 and 2003

Shoot blight of pome fruits caused by Erwinia amylovora, i.e. fire blight, is present in numerous countries of Europe. The disease must have entered into Hungary in the middle of the 1990's and it was first noted and, respectively, identified in 1996 (Bacs-Kiskun county). The losses caused by the pathogen appeared — in orchards and scattered sites of production — in four counties, namely Bacs-Kiskun, Baranya, Bekes and Csongrad at the beginning. From June 1996, a process of eliminating infected parts started in the course of a large action performed under the control of the Department of Plant Protection and Agro-Environmental Economy of the Ministry of Agriculture, under the direction of the plant protection inspectors of the then existing Stations of Plant Health and Soil Conservation. The 'operation' against the disease commenced by cutting back out the infected parts of the canopy and, grubbing them out, respectively. As for the spread of the pathogen (1996-1998) it could be observed that the disease entered into Hungary from the south, south-east and then it also spread into the middle part of the country. As a result of adequate official action and efforts as well as of adequate chemical and antibiotic treatments, moreover because of the introduction of more modern technologies of plant cultivation and those of plant protection it can be reported on that the pathogen hardly appears or does not occur at all on the northern, north-western part of the country. The infection also appears mainly on the parts east of the Danube. Cultivars less susceptible or non-susceptible to the disease are planted in recently established orchards what is also a considerable factor in respect of preventing spread of the pathogen.

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