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The brown rot fungi of fruit crops (Monilinia spp.): III. Important features of disease management (Review paper)
31-49.

In the third part of this review, important features of disease management are summarised for brown rot fungi of fruit crops (Monilinia fructigena, Monilinia laxa, Monilinia fructicola and Monilia polystroma). Several methods of brown rot disease management practices were collected and interpreted in five main chapters. In these chapters, details are given about the legislative control measures, the cultural, physical, biological and chemical control methods. Chemical control is divided into two parts: pre-harvest and post-harvest chemical control. In addition, host resistance and fungicide resistance statuses are also included in this part of the review. Finally, future aspects of brown rot disease control are discussed.

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Mechanical and physical control in apple orchards as preventative fungal disease management
19-21.

In this minreview, mechanical and physical control against apple fungal diseases among non-chemical control approaches were summarized. This overview listed five groups of mechanical and physical control methods:  pruning, removal of inoculum sources, shredding of leaf litter, burying of inoculum sources and flaming of leaf litter. These methods were shown to reduce succesfully infection potential of inoculum sources in orchards and these non-chemical control measures are one of the most essential approaches for preventative fungal disease management. However, most of these methods are not widely spread in the apple-growing practice due to relatively low control efficacy, medium to high labour costs and/or time limits during the season.

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Effect of physical treatments on germination of Ginkgo biloba L.
31-34.

In our country the maindenhair tree (Ginkgo biloba L.) is raised mainly from seeds, so the aim of our experiments was to determine the most useful generative propagation method. However, some experiments have been conducted earlier connected to the germination of the species, but the comparative control of physical seed treatment was done first time by the authors.

After the statistical evaluation of the results it can be stated that the percentage of germination has significantly increased if the seeds received physical treatment (scalding, mechanical scouring). Hereby the pericarp is getting soft or growing thinner, so the germination of the seed is easier. These treatments are extraordinarily simple, easy to carry out and their effect is very favourable, that is why their use is strongly advised.

Comparing the seeds collected at different times was found that the ability of germination is decreasing proportionally with the time spent in the open field. On the basis of our experiments and of earlier practice in Hungarian tree nurseries, our opinion is that the stratification of seeds is not necessary.

 

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Comparison of weed management methods in organic carrot
55-58.

14 combinations of mechanical and also physical (thermal) weed management techniques are compared for organic growing of carrot. Crop of our weed management research is carrot because of its difficulties in weed management (long growing period, poor weed tolerance) and because carrot needs to be important product of organic farming. Herbicide treatment is used as control — cultivator, brush hoe, hand hoeing and hand weeding are mechanical control tools and flame weeder is used for thermal control. Measured parameters are the weed cover, cover of the crop and dry mass of them. Result of the two appraisable years shows contradictory results, which contradiction can be justifiable with different weather conditions of these two vegetation periods. In 2000 brush hoe was significantly the best in interrows but in the year 2001 cultivator combined with hand weeding in rows seemed to show the best result. We can see in this example that agriculture and weed management depends very much on the weather of the year, so that is why it is so difficult to develop a method, which can be generally used for organic weed control of carrot.

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Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars
35-37.

The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.

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High-velocity microprojectile mediated DNA delivery into Phaseolus vulgaris callus cells
99-102.

We report the method for the establishment of rapidly growing callus cultures of Phaseolus vulgaris and the conditions required for efficient transformation using high velocity microprojectiles and high level of transient gene expression. Using hypocotyl explant and vertical culture on B5 medium with lmg/1 kinetin and 2 mg/1 2,4-D, we can recommend to get a rapidly growing callus from bean which is a good starting material to introduce foreign DNA into bean cells. The GeneBooster particle delivery system was used for the bombardment of bean callus and the Hgm resistance gene (Hgmr) was used as a selectable marker gene. 25mg/I hygromycin (Hgm) concentration was sufficient to kill the control callus. We used the standard physical factors, the appropriate pressure of N2 gas for the bombardment of the callus tissue, the shooting distance and the size of tungsten particles used as microprojectiles. Selective and nonselective tests were made by transferring the healthy green and white calluses, subcultured for 4 months on selective and nonselective medium. Several Hgm resistant calli had been obtained. Selective pressure was maintained over a period of 10 months.

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