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Studies on the essential oil of different fennel (Foeniculum vulgare Mill.) populations during onthogeny
Published September 13, 1999

In the recent studies two Foeniculum vulgare Mill. populations (Hungarian and Korean) had been studied in open field trials in 1997-98 in Budapest for the identification of their essential oil characteristics. The essential oil accumulation level as well as the composition of the oil were studied during ontogenesis and at 6 phases of the umbel ...development from budding to overripening.

In the vegetative phases, the accumulation level of the essential oil was higher in the Hungarian genotype. In the leaf rosette stage, anethole is the main compound in both genotypes (40-96%), except the root of the Korean populations which contains 54% dillapiol. The accumulation level of anethole is slightly decreasing, while dillapiol is slightly increasing during the ontogeny. a- and [3-pinenes are characteristic compounds of the leaves, especially in the Korean genotype (10-11%). Before seed setting, fenchone was present in a considerable amount (7.5%) only in the umbels of the Hungarian genotype.

During the generative development, the maximum values of essential oil content are reached at the milky fruit stage (10,11%) in the Hungarian. and at the green fruit stage (7.1% ) in the Korean type, while the composition of the essential oil changed only slightly. The ratio of fenchone is increasing after flowering and being stable during ripening. Anethole varies to a smaller extent in the umbels, only.

We proved, that the dinamics of essential oil accumulation and the oil composition may vary according to genotype. Based on our results, the Korean population is characterized in the system of Bernáth et al. (2) as a high anethol-low methyl chavicol chemoform of the anethol chemovariety (fenchone<15%; anethole>68%; methyl chavicol< 3,2%).


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The influence of nitrogen-fertilizer and harvest time on the productivity of Thymus vulgaris L.
Published August 23, 2000

The influence of nitrogen-fertilizer applied in 0, 50, 100 and 150 kg/ha dosages, as well as the time of the harvest carried out in full flowering and early fruit set stages were studied on the herb and essential oil production of garden thyme (Thymus vulgaris L.). The small plot experiment was installed in the Experimental Field of Ta...rbiat Modarres University near Teheran, under sandy loam soil conditions. On the basis of the results the nitrogen-fertilizer had a significant effect on the dry-matter production of the species: the herb yield, calculated on one hectare, increased from 671.88 kg up to 1021.00 kg value as a result of 150 kg nitrogen dosage. The essential oil yield proved to have a similar tendency because neither the accumulation level of essential oil, nor the ratio of thymol were effected by the nutrient supply. Analyzing the effect of harvest time changes in both dry-mass production and essential oil accumulation were observed. The highest herb yield (1238.20 kg/hectare) was obtained in early fruit set, when about.50 per cent of fruits reached their full size in the inflorescence. The accumulation level of essential oil also reached its maximum at the sane development stage, showing 0.75 per cent value, which is about two fold higher comparing to the accumulation level was measured at the time of full flowering (0.41 %).

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Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
Published February 8, 2006

In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.

In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.

According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.

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Volatile constituents of Nepeta cataria L., N. glechoma Benth. and N. parviflora M. Bieb. from Hungary
Published June 6, 2001

In the temperate zone live about 150 species of the Nepeta genus. Our investigations covered the examinations of the volatile oil containing species of the genus endemic in Hungary, Nepeta cataria and Nepeta parviflora. Latter is a relict of the ancient steppe-flora and endemic in Hungary as well. Phytochemical examin...ation of the volatile oil containing plant material has also been carried out. Catnip growing in the Botanical Garden of PTE Department of Botany contained 0,67% volatile oil in May and 0,14% in November. Chemical character of the volatile oils were measured by gas chromatography/mass spectrometry and citronellol, citral-A, citral-B and geraniol components were identified. The composition of the oil of November samples shifted towards citronellol (65%). In both samples insecticide and repellent activity bearing compounds (+)-cis-p-menthane-3,8-diol, and (—)-trans-p-menthane-3,8-diol in 2-2.5 and 4-4.5% amount have been found. The catnip sample deriving from Germany contained a small amount of anetol, citronellol, neral, geraniol and geranial (6-13%), and possibly two isomers of nepetalactone in 23-31%.

The Nepeta parviflora endemic in the Nagyvolgy valley near Nagykaracsony consisted of the same compounds in the investigated years (1998-2000). Its limonene, methyl chavicol, b-cariophyllene, b-selinene, b-cubebene, davanone, germacrene-D constituents have been identified. In the year 2000 different GC % of these compounds were detected in the different organs of the plants.

The closely related species Nepeta cataria var. citriodora contained 83% citral, and the N. glechoma (= Glechoma hederacea) contained 41% a-cubebene, 20% patchoulenol, 7,7% spathulenol respectively. These compounds were identified by gas chromatography and gas chromatography / mass spectrometry.


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Evaluation of essential oils by gas-chromatography and a new method: "electronic nose"
Published March 21, 2001

The chemical quality of the essential oils is determined by their composition as well as by the complex aroma features. For the evaluation of odour and aroma, sensory tests are practised, especially for applications such as aromatherapy, food industry or perfumery. In the recent studies we investigated eight Mentha origins (populations... of Mentha iperita, M. spicata and M. arvensis) of our genebank collection using a new and effective instrumental sensory evaluation method parallelly with the usual GC analysis.

The results show that the examined mint clones possess different essential oil accumulation levels and special flavour characteristics, too. The GC analysis of the essential oil revealed characteristic differences at both inter- and intraspecific level in the proportion of mentol, menthon, carvon, limonene, menthyl acetate and in two not identified monoterpenes.

In the sensory tests the special complex aroma enables a reliable distinction with a single exception among the populations by the help of the "electronic nose" equipment. The distinction among the samples based on the sensor signals of the instrument — evaluated by multivariate methods — shows a close relation with the detected monoterpene components of the essential oil.


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Utilisation of nutrients by cucumber plants oil rockwool substrate
Published May 18, 2005

Cucumber production by soil less culture on rockwool substrate in Hungary is an open system regarding its water and nutrient supply. Environmental and economical reasons require the recycling of nutrients of the system. Experiments have been planned in order to estimate the utilisation of individual nutrient elements applied. All around the veg...etation period, chemical analyses monitored the depletion of the elements initially administered by sampling the solutions at successive phases from the injection to the overflow. The results have been compared with earlier experiences refering to general rules. It has been stated that the intensity of photosynthesis is decisive in determining the composition of the overflow. The less changes are observed during the period of frequent watering.

The absorption of the nutrient elements varied between 25-51% deperiding on the individual elements. The differences are significant. Further examinations are needed in order to clear:

  • which are the main elements of technology, which are decisive in utilisation of nutrients
  • what are the possibilities of the secondary utilisation of nutrients.
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Comparative analysis of Hungarian Matricaria recutita (L.) Rausch. populations
Published October 20, 2003

Matricaria recutita L. is a traditional medicinal plant in Hungary and its drug is known as „Hungaricum", world-wide. Plant samples and seeds were collected from 12 different habitats of three significant geographical regions of Hungary in 2001. Morphological, production biological and chemical properties of samples were examined. In... relation to the morphological characteristics, a negative correlation (r= -0.75) could be observed between the average height of the plants (height of flowering shoots) and the pH value of the soil. According to the composition of the essential oil, the populations accumulating typically chamasulene (10-20%), a-bisabolol (30-50%) or bisabolol-oxid (30-50%) could be completely distinguished. Concerning the flavonoid composition the quantity of apigenin-7-glucoside was outstanding in the populations originating from the Great Hungarian Plain, it has reached the concentration of 1.8-2.8 mg/g, while the samples collected in Transdanubia could be characterised by much lower level of apigenin-7-glucoside (around 1.5 mg/g).

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Yield and composition of supercritical fluid extracts of different Lamiaceae herbs
Published May 15, 2007

In our work the effectiveness of supercritical carbon dioxide extraction was studied on volatile components of Lamiaceae herbs. The aim of our investigations was to determine the optimal extraction parameters yielding high amount of volatiles in a desirable composition. As plant materials, dried and powdered cude drugs of Thymus vu...lgaris, Thymus pannonicus, Satureja hortensis, Ocimum basilicum, Rosmarinus officnalis and Salvia officinalis were chosen. Pressure (8-30 MPa), temperature (35-60 °C) and time (5-60 min) of extraction were regulated. Results obtained by supercritical fluid extraction (SFE) were compared to that of the conventional extraction procedur, hydrodistillation (HD). In the case of Thymus vulgaris, Thymus pannonicus and Salvia officnalis, extract yield of SFE was comparable to the essential oil amount obtained by hydrodistillation from the same drug. Essential oil rich thyme extracts were analysed by GC-FID. We have found that yield and quality of SFE extracts highly depend on the conditions of extraction.

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Influence of abiotic factors on the green mass and seed productivity of the raw material of Levisticum оfficinalе c. Koch in the conditions of Transcarpathia
Published July 29, 2019

For the wide implementation of Levisticum officinalе C. Koch in Transcarpathia into production it is necessary to widen its sort composition, as it is rather poor in Ukraine. Solving this problem is possible by enriching the source material with the samples of different ecological and/or geographical origin, by studying the influence ...of abiotic factors on the formation of green mass and seed productivity, separating the specimens best adapted to the conditions of growing, involving them into the selection process and creating new, highly productive sorts on this basis. The scientists of the Transcarpathian State Agricultural Experimental Station (TSAES) conducted researches (during 2011-2016) on the adaptive potential of the source material of the type of Levisticum officinalе C. Koch, which counts seven samples (Ukraine – 5 samples, Poland – 1, and Romania – 1), the selection work on creating new varieties for the given region is being done. The investigations were made on soddy podzolic gleyed low-humus soils with low content of nutrients. The results were obtained on the basis of generally accepted methods of investigation, i.e.: laboratory, field, visual, measuring-weighing, biochemical, mathematically statistic, and comparative-calculating. In the conditions of Transcarpathia the spicy greens of the high quality L. officinalе C. Koch are being gathered on days 25-30, the output of the essential oil makes up from 0.72 tо 1.89% on c.d.s., depending on the climate conditions of growing. The plants pass through all the stages of development, the bush is 55.4 to 92.7 сm in height, the plants form a big amount of rosette-shaped stems (11-34 pieces), the productivity of spicy greens is between 9.9 and 42.1 t hа1. The scientists of TSAES created the following sorts – Mriya (А. с. 06131 Ukraine, first registered in the State register of plant varieties of Ukraine) and Coral (Pat. on the variety № 140830). Economic effect from growing of the Coral variety in the conditions of Transcarpathia is 10230 UAH/ha and the level of profitability is 28.4%. The geographic position and agroclimatic conditions of Transcarpathia, as well as the unpretentiousness of L. officinalе C. Koch. tо the conditions of growing contribute to the enrichment of the local flora with useful plants; and the rich chemical composition contributes to the spread of this culture in the given region.

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Yield and composition of supercritical fluid extracts of different Lamiaceae herbs: Satureja hortensis L., Ocimum basilicum L. and Melissa officinalis L.
Published December 8, 2008

In our work the effectiveness of supercritical carbon dioxide extraction was studied on volatile and nonvolatile components of Lamiaceae herbs. The aim of our investigations was to determine the optimal extraction parameters yielding high amount of volatiles and nonvolatiles in a desirable composition. As plant materials, dried and pow...dered cude drugs of Satureja hortensis, Ocimum basilicum and Melissa officinalis were chosen. Pressure (8-50 MPa), temperature (35-60 °C), time (5-60 min) and the ratio of modifier (5-50% methanol) of extraction were regulated. Results obtained by supercritical fluid extraction (SFE) were compared to that of the conventional extraction procedure, hydrodistillation (HD). In the case of Satureja hortensis, Ocimum basilicum and Melissa officinalis extract yield of SFE was comparable to the essential oil amount obtained by hydrodistillation from the same drug. Essential oil rich extracts were analysed by GC-FID, while extracts rich in nonvolatile compounds were analysed by HPLC. We have found that yield and quality of SFE extracts highly depend on the conditions of extraction.

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Evaluation of supercritical plant extracts on volatile and non volatile biologically active lipophil components
Published June 6, 2001

Authors dealt more than ten years with the analysis of supercritical extracts. For extraction (SFE) carbon dioxide was used as supercritical solvent. Fractionation of extracts was carried out by releasing the separations pressure at two stages. The extracts were collected as separate samples successively in time.

The traditional extract...ions were carried out with steam distillation or by using n-hexane and ethanol in Soxhlet apparatus. For the analysis of volatile compounds GC, GC-MS; of non volatile compounds TLC-densitometry and spectroscopic methods were used.

The following general characteristics were established comparing the composition of steam distillated oils with that of volatile SFE fractions. The SFE fractions were richer in monoterpene-esters and poorer in alcohols than the essential oils prepared by traditional way (clary sage, lavandel). Regarding the distributi,n of the monoterpene and sesquiterpene compounds, the SFE fractions contained sesquiterpene hydrocarbon in higher percentage than the distillated oils (e.g. 13-caryophyllene in Salvia fruticosa, (3-caryophyllene, y­muurolene, y-cadinene in Ochnum basilicum). Further the proportion of sesquiterpenes increased in SFE fractions collected successively in time.Significant difference was remarkable in respect of the optical rotationability of lovage oil and SFE fraction which was probably caused by the different ratio between the two ligustilid enantiomers. It was verified in some cases that a part of mono- and sesquiterpenes were present originally in a bounded form (glycosides) in plants. Therefore they appeared in essential oil fractions only after previous acidic treatment (Thymus, Origanum species). During the supercritical extraction the azulenogene sesquiterpene lactones did not transform to azulenes (in chamomile, yarrow), but the non volatile SFE fractions of some Asteraceae plant contained sesquiterpene--lacton of unchanged structure in high quantity (e.g. cnicin in blessed thistle, parthenolide in feverfew). Authors obtained also SFE fractions which were rich in triterpenoids and phytosterols (marigold, common dandelion).


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Sensory analysis as a supporting method for marjoram breeding
Published June 6, 2001

Marjoram is one of the most important spices in Hungary, which is utilized both in phytotherapy as well as in alimentary industry. Organoleptic quality of the drugs of two Hungarian varieties of Majorana hortensis /'Francia' and 'Magyar'/ were investigated by two different methods of sensory analysis. The chemical composition of the ma...terial was checked by GC analysis of the distilled essential oil. Although their main compounds and proportions showed similar patterns, both the human and instrumental sensory tests based on the complex odour and aroma of the drug, proved a significant differentiation of the two varieties.

On the basis of our results, sensory evaluation may be a useful tool in the practice in determination of complex aromatic values of marjoram, as a spice. At the same time it seems to be a suitable method in promotion of breeding efforts.


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