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• Analyses of Hungarian sour cherry germplasm with simple sequence repeat markers

  27-31.

  Z. György

  T. Szabó

  J. Nyéki

  A. Pedryc

  Views:

  198

  Twenty-four sour cherry cultivars (genotypes), belonging to four cultivar groups were fingerprinted using microsatellite markers. All genotypes have been arisen from the Carpathian basin, which could be secondary gene centre of sour cherry, since its progenitor species, ground cherry and sweet cherry overlap here. Five SSR primer pairs, earlier used for fingerprinting Turkish sour cherry germplasm were tested. None of the five primer pairs showed any polymorphism within the cultivar groups. The primer pairs were able to distinguish between the cultivar groups. The Oblacsinszka and the Cigánymeggy cultivar groups were the most difficult to separate, while the Pándy cultivar group was the most distinguishable.

• Molecular identification of old Hungarian apple varieties

  37-42.

  B. Wichmann

  Zs. Galli

  S. Molnár

  Zs. Galbács

  E. Kiss

  T. Szabó

  Heszky L.

  Views:

  242

  Altogether 40, mainly old Hungarian apple varieties were screened with six previously described microsatellite markers. A total of 71 polymorphic alleles were detected (average 11.8 alleles/locus) and the heterozygosity of markers averaged very high (0.8). The genetic variability among the genotypes proved to be so remarkable that as few as three markers from the applied six were enough to distinguish between the 40 varieties. This was also confirmed by the cumulative probability of obtaining identical allele patterns for two randomly chosen apple genotypes for all loci, which value was quite low: 2.53 x 10^-5. The molecular identification of these genetically very different old apple genotypes could be very useful in future breeding programs.