A collection of 151 apple cultivars was investigated with 7 RAPD primers generating 71 informative bands, to evaluate genetic variability and relatedness. All cultivars presumably derived through genetic recombination were distinguished whereas identical DNA profiles indicated that some cultivars had arisen as sports. A cluster analysis and a P...CO did not reveal any distinct geographic patterns, but there was a weak tendency for Swedish and foreign cultivars to differentiate.. Many cultivars however clustered together with either one of their parents or with siblings. Overall genetic diversity among the 151 cultivars was estimated with Nei's diversity index (H), 0.269, and with Shannon's index (H'), 0.594. The cultivars were also analysed in six groups, according to time of origination and country of origin, with an average H = 0.262 and H' = 0.546. No major differences in genetic diversity were observed over time or space, although the group with recent, foreign cultivars had the lowest diversity (FL = 0.235, H' = 0.493). Comparison between the entire material and a subset with 94 mandate cultivars chosen for preservation in Sweden, showed similar genetic diversity: HFN.rj1E = 0.268, frE,NTIRE, = 0.593 and HMANDATE = 0.263, WMANDATE = 0.575. No major differences in band frequencies were observed between these two sets, but 5 RAPD bands were missing in the set with mandate cultivars.
Quince (Cydonia oblongaMill.) is a minor fruit crop, which is primarily used for marmalade, jam and sauce.Very few quince cultivars are known all over the world and in many cases similar names are used for presumably different cultivars. The aim of the present study was to evaluate and characterize the genetic diversity of 36 quince cultivars a...nd selections with SSR markers. Seven out of 8 SSR markers designed from apple sequences could successfully yield amplification also in quince cultivars. Number of alleles per locus ranged from 2 to 3 alleles. These allele numbers are quite low when compared to apple. It is supposed to be the consequence of a genetic bottleneck. In spite of the low allele number per locus, the 36 quince cultivars formed 30 different genotypes. The ratio of homozygosity was low, which might be coupled with the self-(in)compatibility phenotype of quinces. SSR markers proved unable to differentiate putatively closely related cultivars (e.g. ‘Bereczki’ and ‘Bereczki bôtermő’). In general, the level of polymorphism among the tested quince genotypes was much restricted due to the low allele number detected. However, it must be considered that the number of analysed SSR loci is not enough high to estimate the overall heterozygosity of the quince genome. Further experiments are needed and the SSR markers proved to be a reliable and useful tool for such analyses.
Apricot takes an important place in Hungarian fruit production. Considering morphological characteristics of apricots it was concluded that the genetics background of European cultivars is very limited. Molecular markers and their use for genotyping have revolutionized the identification of cultivars. In a classic apricot breeding program, it i...s important to be able to establish unique DNA profiles of selections to identify them unambiguously and to determine their genetic relationship. Presently SSR is far the most frequently performed technique for genetic diversity studies. In this study there were used peach and apricot primer pairs from four different sources in order to examine microsatellite polymorphism among cultivars and investigate relationships among them. The possibility of cross species amplification among different Prunus species using SSR primers allowed us to use primers developed in peach to study genetic diversity in apricot. In this work, 90% of the primers used were able to amplify SSRs in apricot and more than half of them were polymorphic. With the 10 primer pairs utilized were proven to be sufficient to set unique fingerprint for several cultivars studied. The obtained dendrogram classified of the 45 cultivars included in this study into two major groups and several subgroups.
TheHungarian pear collection (Pyrus communis L.) consists of 423 genotypes distributed over seven genebanks inHungary. This is one of themost extensive collections of native and cultivated pears found in Eastern Europe and includes a wide range of genotypes with small size fruit (referred to as “Miniature pears”). Based on the in situ and e...x situ measures taken by governmental and other institutions for fruit tree conservation in Hungary, an overview is given on some activities regarding areas of Pyrus collection and genebanks where pears are collected and grown. Descriptions of traits of miniature pears found in Hungarian genebanks for the interest of genetic characterization and breeding are presented.
The principle task of the sustainable development is the preservation of the genetic variety, which is similar challenge in the horticulture regarding the sublimation of fruit species. The breeders of the traditional fruit strains give stock to the sustenance diversity of the agro-environment on the species and landscape level. In 2009, hypersp...ectral images have been taken by AISA Dual sensors from the pear gene pool in Újfehértó, Hungary. The hyperspectral data cube (in the wavelength range of 400-2500 nm, with 1.5 m ground resolution) ensured possibility to make the spectral library of pear species. In the course of the simultaneously field work the spatial position and individual extent of all pear trees was defined to set up a detailed GIS data base. The water stress sensitivity of single species and the descriptive spectral curves were determined with common evaluation of the spectral and spatial data. Based on the unique methodology processing and the hyperspectral data base suitable strains can be chosen for agro-environment and let take adaptive stocks regarding climate change into the genetic grafting work. Furthermore we could determine and map the sparsely species in the region with the help of the hyperspectral data.
Rhodiola rosea L. is an important adaptogen medicinal plant. In this study two new microsatellite markers were developed. The assessment of the genetic diversity of R. rosea has recently started with molecular markers, but only a few species-specific microsatellite markers have been published so far. However the small number of markers allows o...nly a limited insight into the genetic variability of the species therefore the aim of our work was to develop new microsatellite markers for R. rosea with a microsatellite enrichment library technique. Genomic DNA was cleaved with an endonuclease enzyme followed by adaptor ligation and PCR amplification. DNA fragments that contained microsatellites were first isolated using a biotin-streptavidin linkage based magnetic selection and then cloned into plasmids. Out of forty-three sequenced clones three contained microsatellites, in these cases primers were designed for the amplification of the microsatellite repeats. The newly developed primer pairs were tested on individuals from distant R. rosea populations and the variability of the amplified fragments was estimated by fragment-length analysis. The locus RhpB14a was found to be monomorphic while RhpB14b and RhpB13 were polymorphic. As a result of the present study, two novel variable microsatellite loci were identified in the genome of R. rosea.
RAPD markers were used to reveal genetic diversity between nine varieties of Cucumis melo L. and to identify the studied varieties. Of the 60 primers tested 12 primers produced polymorph patterns. A set of 4 primers was sufficient for distinction the nine investigated melon varieties.
The widespread genus Cotoneaster has its centre of diversity in the Himalayas and surrounding areas. Most taxa appear to be polyploid and apomictic, and many of them have become popular ornamentals due to their attractive foliage and berries. One of the taxonomically most critical groups is Section Alpigeni which contains seve...ral important ornamental plants. The number of species belonging to this section varies widely between different taxonomic treatises, depending on whether 'splitting' or 'lumping' of species is preferred. Using a rather narrow species definition, we have investigated 13 different species using RAPD analysis. A simple matching (SM) coefficient-based principle coordinate analysis (PCO) was calculated from the RAPD data. Some species were clearly more similar to each other than to other species in the analysis. The levels of similarity did, however, not correspond very well to the lumping together of several taxa under the same species name as performed e.g. in the recent Flora of China. Obviously, the complex hybridogenous origination and, in some cases, still ongoing recombination with sexual species or among the apomictic taxa themselves, produces a genetic variability structure that cannot be properly reflected in a hierarchical taxonomy.
Blackspot, caused by the fungus Marssonina rosae (Diplocarpon msae), is one of the most devastating and widespread diseases in garden roses, and it has as yet not been fully characterized in molecular terms. In this initial study we used RAPD analysis to investigate the genetic diversity among and within a few geographically diverse gr...oups of single-spore isolates of M. rosae. DNA was extracted from in vitrogrown mycelia of 1 I single spore isolates grown on PDA medium. High levels of polymorphism were detected among the isolates. They clustered into three distinct groups: Group !consisted of isolates from eastern North America plus a European isolate (Germany), Group 2 included isolates from southern Sweden, and Group 3 included the isolates from Manitoba, Canada. The greater similarity of the environmental conditions in eastern North America and Europe as compared to the Canadian prairies suggest that. climate and weather could be key factors in influencing the potential race structure of M. rosae. However, variations among closely situated sites, e.g. southern Sweden, also occurred.
Almond [Prunus dulcis (Mill.) D. A. Webb.] as one of the oldest domesticated plants is thought to have originated in central Asia. Gametophytic self-incompatibility of almond is controlled by the highly polymorphic S-locus. The S-locus encodes for an S-ribonuclease (S-RNase) protein in the pistils, which degrades RNA in self-pollen tubes and he...nce stops their growing. This study was carried out to detect S-RNase allelic variants in Hungarian and Eastern European almond cultivars and Turkish wild growing seedlings, and characterize their S-allele pool. Five new alleles were identifi ed, S31H, S36-S39 in Eastern European local cultivars. The village Bademli and Akdamar island are two distinct places of almond natural occurrence in Turkey. Trees growing wild around Bademli city showed greater genetic diversity than those originated on Akdamar island. Many of the previously described 45 S-RNase alleles have been also detected in these regions. Homology searches revealed that Turkish almonds carried some P. webbii alleles indicating hybridization between the two cultivars and massive introgression events. Our results supply long-awaited information on almond S-allele diversity from regions between the main cultivation centres and the centre of origin of this species; and are discussed from the aspect of methodological developments and evolution of the cultivated almond.
European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the secon...d intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.
As it well known the decrease of biodiversity is a large problem all over the world. In case of medicinal plants, where the huge majority of drugs are collected from natural ecosystems, the sustainable, utilization of the populations and reservation of their gene-pools has an increased interest.
In Hungary, the major background of 'in s...itu' reservation of medicinal plant species, their natural plant associations and ecosystems is the official protection by law. Successful examples are known for the controlled utilization of medicinal and aromatic plant species grown in protected areas. Assuring the naturally occuring high degree of biological and chemical diversity of species is a special task in this field: only maintenance of valuable intraspecific races can form the real genetic basis of natural biologically active compounds.
Maintenance of chemotaxonomical gardens and gene bank collections (seed banks, tissue banks) as 'ex situ' methods of reservation is carried out on an extended range in Hungary. As the required information on storage and maintenance of many medicinal and aromatic plant species is yet missing, exhaustive research is carried out at both genebanks in Hungary, which are specialized for medicinal plant reservation (RIMAP- Budakalász, SZIU- Budapest).
Beside the static conservation methods, 'quasi-production systems' are intended to assure an up-to-date and economically possible way of dynamic reservation with sustainable utilization.
Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such... as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.