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Study of genetic resources for Powdery mildew resistance by biochemical and physiological parameters on peaches and nectarines in Romania
Published March 16, 2004

During 1997-2002, studies on the physiological and biochemical changes due to powdery mildew attack in peach and nectarine cultivars and hybrids belonging to different classes of resistance had been performed at the Research Station for Fruit Tree Growing (RSFTG) Baneasa Bucharest, located in the southern part of Romania. The study was based on... disease assessments in the field and on other diagnosis criteria.

The southern part of Romania has favourable ecological conditions for growing peach and nectarine but trees have been attacked by various pathogens such as Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. persicae Woronichin fungus for a long time. The presence of the pathogen causes important changes in the quantity and quality of fruits and affects the metabolism of trees.

This study was focused on several physiological and biochemical parameters such as photosynthesis and respiration intensity along with catalase activity; and changes in some biochemical compounds such as free, total and linked water content, dry matter, nitrogen and protein content, tannin levels. The aim was to find possible correlation among the above parameters and the biotic stress induced by the powdery mildew fungus calculated as attack degree (AD%).

Results showed that photosynthesis diminished significantly in the case of the attacked phenotypes, the respiration was more intense in the infected cultivars and catalase proved to be less intense in the affected plants. The total water content significantly increased in contrast to the dry matter and tannin contents, which greatly reduced in the case of resistant cultivars such as 'Victoria', Vectared 7', 'KB 11-40'. The total nitrogen and protein levels showed less influence on resistance to powdery mildew on all cultivars. All genetic material found resistant to powdery mildew is currently used in peach and nectarine breeding programs in Romania.

Further studies focused on isolation and characterization of resistance genes for powdery mildew resistance will be done in the future based on data collected during several years.

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The effect of different berries in human nutrition
Published September 19, 2007

The aims of this study were on the one hand to compare of examined compounds according to their importance in nutrition and human-health, and on the other hand we made preexperiments to investigate the relationships among antioxidant capacity and the endogenous substances which contribute the antioxidant status of the plants. The following spec...ies were involved in the experiment: raspberry, blackberry, black currant, elderberry and sour cherry. These fruits have potent health-promoting antioxidant power. Glucose, fructose, total phenol, formaldehyde and anthocyanin contents were determined in addition to ferric reducing ability. Our results reflected considerable differences in the measured parameters of the analysed species. In blackberries and elderberries the high antioxidant capacity is coupled with low carbohydrate content. Besides the formerly proven correlations between total phenol content, anthocyanin concentration and antioxidant capacity, these parameters also correlated with the measured formaldehyde concentrations, hereby we can follow the methylation /demethylation status of the plant.

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Preliminary biochemical studies on a model growing of different tobacco plant (Nicotiana tabacum L.) cultivars
Published May 15, 2007

The aim of our preliminary studies was to characterize the change in the activity of enzymes PPO and PDX, the concentration of total soluble phenolic compounds and soluble protein content of different tobacco cultivars measured in tobacco plants during cultivation. The results suggest that a shorter cultivation period (13-14 weeks) is ...more favourable for tobacco plants as protein source than for tobacco industrial use (16-17 weeks). We found some correlation between the concentration of total soluble phenolic compounds and PPO activity data. In the increasing and maximum period of total phenol content the PPO activity was high. But later because of decrease of substrate phenol content the activity of PPO also decreased. We found higher soluble protein concentrations in Virginia than in Burley varieties.

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