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Results with the establishment of in vitro culture of Leucojum aestivum
67-71.Views:217Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too.
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Histological studies on small bulb flower crops
83-85.Views:128Investigations were done in order to obtain more information on the histological structure of bulbs of Allium moly, Allium sphaerocephalon, Muscari armeniacum and corms of Crocus sativus. While starch grain were found in the bulb of Muscari armeniacum, fluid nutrient content were shown in Allium species. Narrow and fleshy scales were found in the bulbs of Muscari. Histological characteristics of Allium moly and Allium sphaerocephalon showed a closer relationship with corms than with the bulbs. To clarify this question, further investigations are needed (Bailey, 1942; Larson, 1980).
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Histological studies on some native perennials
79-82.Views:124Growing of native perennial species became more and more popular in the last ten years. In order to obtain more information on their histological structure, investigations were done on Aster linosyris, Inula ensifolia and Prunella grandiflora. The histological features are usually relating to the plants' ecological demands which is an important aspect in their growing. Differences were found in the structure of the stem of Asteraceae and Lamiaceae members. While separated vessels were formed in the stem of Aster linosyris and Inula ensifolia, continuous vessel-system forms in the stem of Prunella. Alternating segments of collenchyma and chlorenchyma are found in the stem of Aster linosyris, while palisade parenchyma is situated both on the abaxial and adaxial surface of the leaves. Vessel-system of the root is tetrarch. Histological structure of the stem of Inula ensifolia differs from Aster linosyris in the broader cortical parenchyma which is composed of approx. 8-12 cell layers. It contains neither collenchyma nor chlorenchyma. In the stem of Prunella grandiflora a nearly continuous vessel-ring is formed from the four primary vessels. Long, multi-celled hairs were observed in the district of angles of the stem.
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The effect of Ferbanat L nano-fertilizer on the growing of Petunia x grandiflora ’Musica Blue’
107-109.Views:241During our experiment, the effect of Ferbanat L concentrations were examined by Petunia x grandiflora ‘Musica Blue’ production. The leaf and shoot length, number of flower buds, diameter of flowers, and the date of appearing the first buds were measured. The solution of Ferbanat L in 0.1 % concentration was the most effective on shoot length (21.7 cm). Remarkable increase was observed by the other treatment groups as well comparing to the control group (5.6 cm), the plants treated with solution of Ferbanat L 0.2% (16.5 cm) and 0.3% (14.4 cm) had significant effect as well. The nano-fertilizer had not positive effect on the other examined parameters as leaf and flower size, number of flower buds or chlorophyll content. The culture period shortened five days.