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Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods
37-42.Views:163Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.
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Impact of substrate supplemented with CaCO3 on mycelial growth, yield, morphological features and storability of fruiting bodies of black poplar mushroom Agrocybe cylindracea (DC.) Marie.
76-86.Views:354Black poplar mushroom, Agrocybe cylindracea deserves special attention, due to its medicinal properties. Water and alcohol extracts from fruiting bodies of the fungus have an anti-oxidant, anti-cancer, anti-fungal, cholesterol and triglycerides blood level lowering abilities. This mushroom is rich in proteins and vitamins, mineral elements and low in fat. The aim of the experiments was to determine effect of a substrate supplementation with CaCO3 on mycelial growth, yield, morphological features and storability of fruiting bodies of four strains of A. cylindracea (DC.) Marie. The amount of additive to sawdust substrate affects rate of mycelial growth and yield of investigated strains. A. cylindracea mycelial growth was not affected by addition of CaCO3 to substrate, however a significant effect of this additive was found on yield, which was the highest with CaCO3 addition in an amount of 8 g/100 g of substrate. Carpophores characterized with the largest caps diameter, and the largest individual mass obtained of substrate enriched with CaCO3 addition of 8 g/100 g of substrate. In addition, it was found that supplementation with CaCO3 affect storability of A. cylindracea. The lowest weight loss of fruiting bodies after 3 and 7 days of storage was found with addition of CaCO3 to substrate in an amount of 4 g/100 g of substrate.
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Influence of irrigation and row spacing on the growth, seed yield and active substance of milk thistle (Silybum marianum)
78-81.Views:198Milk thistle (Silybum marianum (L.) Gaertn) has been used in medicine since ancient times, but it has been cultivated as a medicinal plant only in recent decades. The ripe fruit of milk thistle contains flavonoids, which are used to prepare anti-hepatotoxic drugs. The main purpose of this study was to substantiate the effects of irrigation and row spacing on growth, seed yield and the content of active substances (silybin and silymarin) in milk thistle. The results showed that the suitable amount of irrigation was 20 mm and the appropriate row spacing was 25 cm.
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Comparison of Xanthomonas arboricola pv. juglandis isolates from walnut trees grown in Romania and Hungary
65-69.Views:262Among diseases that affect walnut, bacterial blight is considered the most important one in all walnut growing areas both from Hungary and Romania. For the determination of susceptibility/resistance of walnut cultivars in our posterior work planned, 61 bacterial isolates were collected from walnuts showing symptoms of blight, with the purpose of isolating Xanthomonas arboricola pv. juglandis (Xaj). The characteristic Xaj colonies, frequently present as saprophytes in the infected plant tissues, were separated from other bacteria according to their morphology, yellow colour, hydrolysis of starch, and oxidation of glucose. All isolates were tested for pathogenicity by hypersensitive reaction on tobacco leaves (Nicotiana tabacum L.), bean pods (Phaseolus vulgaris L) and unripe nuts (Juglans regia L.). Determination of taxonomy of the selected isolates denotes a possible subdivision (races, biotypes) of Xaj occurring in different geographical areas, API 20NE and API 50CH kits were used. Hungarian and Romanian strains showed a high degree of similarity of carbohydrate utilization but slightly differed from the type strain. All were Cu-sensitive.
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The effect of drying process on the pigment content and composition of table beet varieties
115-117.Views:175The food industry requires natural colouring agents in increasing quantities. Beet root is highly adapted to this purpose with its red (betacyanin) and yellow (betaxanthin) pigments suitable for numerous products. The two pigments are, however, very heat-sensitive depending not only on the method of drying as well. In our experiment we tested 6 table beet varieties with vacuum drying (instrument: Eurovac KIT-150) and obtained very big differences in the suitability of varieties for drying. The less colour loss was measured in Bordó (16.84%), while Rubin and Cylindra indicated 45.15 and 47.18% loss respectively. During the drying raw material with higher colour content produced higher rate of pigment loss (r = 0.880). In our experiment we found adverse effects on colour material and dry matter content in the fresh beet root. The variety with higher solids (mainly sugar) resulted in higher pigment loss (r = -0.847) during the vacuum drying process. Furthermore, we stated that the yellow pigments (betaxanthin) were less heat sensitive during drying than the red (betacyanin) ones. For the production of beet root powder varieties of high pigment content but low water soluble solids content (sugar) are needed.
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Organoleptic evaluation of pear varieties grown at different sites
33-37.Views:225It was stated as a summary that a system of testing pear varieties on scale of 100 degrees, weighing 11 properties, gives a comprehensive information on qualities of pear varieties. The points of the form to be filled out, distinguishes the appreciation of accessory flavours and tastes from the main properties. Under the title of taste, sweet and acid taste are evaluated separately. The presently used system is a step forward as the composed characters do not influence the judgement of each other. In the representation of results, the 100 degree system was more efficacious than the usual 5-point system because there was no need of using fractions, and the graphic profile is more expressive, so the commodity is better visualised. The assembly of the jury was divided into two groups as below and above 35 year old persons. The two groups judged differently the acidity, sweetness and the thickness of the fruit peel. It was suggested that the choice of the produce should consider the differences in taste and give up the idea of being equally favourable for both groups. The samples of different growing sites revealed that the best quality is produced at Nagykanizsa, and this capacity would be able to supply the market also in the large super-markets and chain of department stores in Hungary.
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Mechanical and physical control in apple orchards as preventative fungal disease management
19-21.Views:249In this minreview, mechanical and physical control against apple fungal diseases among non-chemical control approaches were summarized. This overview listed five groups of mechanical and physical control methods: pruning, removal of inoculum sources, shredding of leaf litter, burying of inoculum sources and flaming of leaf litter. These methods were shown to reduce succesfully infection potential of inoculum sources in orchards and these non-chemical control measures are one of the most essential approaches for preventative fungal disease management. However, most of these methods are not widely spread in the apple-growing practice due to relatively low control efficacy, medium to high labour costs and/or time limits during the season.
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The Role of Human Factor in the Innovation of Apricot Production-Empirical Study in a Disadvantage Area of East-Central European Country
131-134.Views:203Hungary is a traditional fruit growing country for ages. As fruit sector has a very high hand work request and value added, it has an important role to decrease the elimination of unemployment and the lack of income in the disadvantage rural areas. The study was made in the year of 2009, the studied population consisted of the members of the fruit-grower marketing organization (Gyümölcsért Ltd.), that organizes growing and sales of stone fruits in Hungary. The number of studied population were 95 capita, the number of fi lled out and evaluated questionnaires was 35. By the composition of the questions both qualitative and quantitative methods have been used. We tried to get answers to the following questions: Are the studied human factors (age, educational level, sex etc.) of growers, determined the extension of innovation of apricot production?
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Studies on the drought responses of apple trees (Malus domestica Borkh.) grafted on different rootstocks
29-36.Views:369The morphological and chemical changes in the leaves of eight apple varieties grafted on M4, M26 and MM 106 rootstocks were examined during fruit development in non-irrigated plantation. The aim of the experiment was to examine the defensive mechanisms in apple trees under the mild and serious dryness occurring during fruit development. The content of a relative chlorophyll (SPA D value) of the apple leaves on eastern side of the trees was lower than western and southern sides under drought occurring du ring fruit devclop111ent Under sustained drought from first of July to August the SPAD value, the weight of leaves were larger and the stomata density was significantly larger than in the previous periods. There was a close positive significant correlations bet ween SPAD and stoma density both in the group with less sensitive to drought (r=0,8429) and in the large water demanded group (r=0,9209) equally. The rate of increase in SPAD values and the stoma density was slower in the group with drought tolerant, than in the group required good water supply. The varieties being sensitive to water deficite such as Akane. Pink Lady and Red Rome van Well have a l ready responded intensively to short time of drought by the increasing of carbohydrate and antioxidants product ions. At 42 days of sustained drought as soon as the level of carbohydrate in the leaves decreased, the antioxidant s contents of the leaves rised suddenly in the apple varieties with moderate water requirement s as Greensleeves and ldared. Gala apple variety seemed to be able to defend the drought for longer time because there was no change in relatively high level of carbohydrates and antioxidants of their leaves under severe drought during fruit development.
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Influence of strawberry and raspberry consumption on the antioxidant status of human body
109-113.Views:141Several parameters influencing the antioxidant capacity were measured in our experiments to compare strawberry and raspberry cultivars. The analysed cultivars were: 'Honeoye', 'Elsanta', 'Onebor (strawberries) and 'Glen Ample', Fertődi zamatos', 'Mailing Expoit' (raspberries). Besides the determination of dry matter content the content of glucose, fructose, total phenol and anthocyanine, the reducing power, H-donating activity and total scavenger capacity were measured as well as the compound of mineral nutrients. Among the species and the cultivars, differences were obtained. These results call the attention to the features of cultivars and to count on the antioxidant-capacity describing values in case of consuming these fruits. In early spring and summer the consumption of strawberry and raspberry has a beneficial contribution to the antioxidant status of our body. In this respect these are more valuable than the year-long consumable apple. Accordingly, our measurements showed higher amount of sucrose, phenolic compounds and anthocyanine in raspberries, but strawberries contained more glucose and fructose and had stronger H-donating activity. It is worth to keep in mind that e.g. which cultivar contains more of certain compounds because of the strictly determined allowable dose in some diseases (e.g. diabetes).
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Evaluation of precision farming with regard to horticulture
105-110.Views:119The global positioning system was made available for public use, which made it possible' to apply a new management tool in agriculture. Precision farming gives much more information on plant-growing than former methods, which makes it possible to use technologies more suitable for micro-sites. It is supposed that more profitable production can be realised with its aid and the strain on the environment can be reduced, not to mention other economic advantages.
The study makes economic conclusions about the method more and more widely used in plant production taking the yield mapping of Józsefmajor Experimental Farm as a basis and starts ideas about its possible application in arable land olericulture, viticulture and fruit production.
These issues are important because precision farming has existed in glass-culture for a long time from another perspective, which should be spread to open ground horticultural enterprises that are labour and asset intensive, qualitative farming forms with great plantation value.
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Training systems of fruit trees in Hungary
123-127.Views:283Growing sites and soil conditions of Hungary warrant profitable production of several temperate fruits at elevated levels of quality. The climate of the Carpathian basin is a mixtures of three main climatic zones the prevalence of which may change seasonally: Atlantic, continental and Mediterranean, therefore, growing sites are rather various. Temperature minima of the winter and late spring frosts are the main elements of risk. In choice of the system of cultivation, regularity of yields and intensity are to be observed equally. Regular yields are particularly aimed in stone fruit cultures.
For apple and pear plantations of high density required for intense production are promoted favourably. Accessories of intense orchards (irrigation, supporting system, rootstocks, phytotechniques, etc.) are important. In peach and plum trees are trained to funnel-shape crowns, in general, intense-types are possible in plum, only. In apricots, a Hungarian speciality, the "umbrella" type of crown is applied, almost exclusively, according to Papp. In sweet and sour cherry, the harvest technique, manual or mechanised, according to the intended utilisation, are determining the form of training.
Red and black currants as small fruits are grown mostly as bushes or hedgerows without any supporting system designed to facilitate mechanical harvest. Raspberries and blackberries are grown as hedges on trellis. Gooseberry is a special case, being a low, thorny bush difficult to be picked. Thus grafted small trees are attached to a wire-trellis which helps to solve problems of plant protection too.
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Apricot fruit chilling injuries during the cold storage affected by harvest maturity
28-31.Views:230The study aimed to find the effect of different maturity classes (up to the days after full blossom) on the post-harvest life of 3 different apricot varieties Gold Cot, Bergarouge and Flavor Cot in Hungary. The fruit harvested in a particular time 65, 75 and 85 days after full blossom and divided to three maturity classes (Class 1, 2 and 3, respectively). Fruit stored in cold storage for 7 days at temperature 1 °C. Fruits were investigated in regard to physical parameters (weight loss, fruit firmness and soluble solid content SSC) and chilling injuries. The results showed that the varieties followed different ways in regard to response to different maturity classes. The maturity class I for all the tested varieties recorded the lowest weight loss, while with the increasing maturity stage the weight loss percentage for all the varieties recoded high values. The firmness decreased with delayed harvesting for all the varieties. Data of the chilling injuries showed that all the fruits which harvested at maturity I, recorded the highest percentage of fruit with CI (chilling injuries) at 0 (48.5, 37.25 and 38.75%) and CI class I (44.75, 35.75 and 39.75%) for Bergarouge, Gold Cot, and Flavor Cot.
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Flower density and winter damage of apricot and peach varieties
53-56.Views:223Hungary is located on the northern boundary of economical apricot and peach production. The present assortment of varieties and the actual, not adequately selected growing sites cause a permanent risk of winter and spring frosts in their cultivation. The field observations are performed at Debrecen, the Experiment Station Pallag, on 20 apricot and 21 peach varieties. The flower density among the varieties attained 3-4-fold differences. Three categories have been suggested for both species. The density was inferior in Hungary established varieties compared with the new varieties of foreign origin. The minimum temperatures of January 9, 2009 was –17,6 °C , and of December 21, 2009 also –17,6 °C. In some varieties the damage of buds attained 100%. For estimating the yield security, we need to consider also the flower density and the frost damage together. For a mediocre yield, we need a flower density in both species of at least 0.2 living bud/cm. The results confirm the statement that in Hungary, the revision of growing sites is indispensable in order to develop a profitable and competitive apricot and peach growing industry.
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Cultivation systems in plum orchards
125-129.Views:248In this review, we give an overview of recent cultivation systems in commercial plum orchards. The aspects of selecting the shape of the crown and the spacing of trees are discussed first and then growth characteristics and the structure of the crow are described in detail. Finally, the wound healing and regeneration characteritics of plum trees are detailed which have an influence on the applied pruning technique.
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Mechanical behavior of catching surfaces used for fruit harvesting
59-64.Views:104Various fruit gathering devices are important part of tree shakers used in fruit harvesting. The catching surface is generally used in the form of a stretched thin sheet interacting with the falling fruit. This interaction depends on many factors such as the shape and mass of the fruit, the falling height and the mechanical properties of the catching material.
In recent investigation different canvas, composite and silk materials were selected and their mechanical behavior was experimentally determined. Using the energy conservation principle, the main impact characteristics of the falling fruit was derived and used. Static and dynamic loading tests were carried out using spherical intenders and artificial fruits. Investigations have shown that, using the appropriate material characteristics, the catching surface can serve as a cushioning material and it can considerably reduce the impact stresses in the falling fruits.
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Possibilities and limits of use plastic constructions in fruit growing technologies
71-75.Views:214On the Experimental Station Pallag of Debrecen University different combinations of fruit species and rootstocks have been raised under and without plastic foil cover in 2002. The growth and productivity of the grafts was our objective of comparison. Results revealed substantial differences in fruit set and yield depending on species and varieties. Sweet and sour cherry varieties grew much shorter under the plastic cover, whereas apricots, peaches and plums set fruit much more eagerly compared with the trees outside the plastic cover. Outside the plastic cover, the trees were much more developed at the beginning of their fruiting period. In spite of that, the growing processes were more intense under the foil. The differences are allegedly due to the repeated summer pruning necessary under the restricted space of the foil, on the other hand, due to the root concurrence because of the dense planting. Regarding the inner properties of the fruits, soluble solids, sugar and acids were higher outside, whereas macro- and mezzo-elements (P, K, Ca, Mg) were more abundant in fruits grown under the foil cover. Further efforts to explore those relations with other varieties and rootstocks are justified.
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Terminology of fruit set and fruit drop of sour cherry cultivars
33-36.Views:201Fruit set and fruit drop rates of 9 sour cherry cultivars (‘Érdi bôtermő’, ‘Debreceni bőtermő’, ‘Kántorjánosi’, ‘Újfehértói fürtös’, ‘Éva’, ‘Petri’ ‘Oblocsinszka’, ‘Pandy 279’ and ‘Csengôdi’) of eight years-old trees grown in Újfehértó, located in the Eastern north part of Hungary which grafted on Prunns mahaleb have been studied. Significant differences have been found in fruit set among cultivars. The average percentage of fruit set was 18.3%, which the ‘Oblacsinszka’ by 32.6% the highest fruit set, while ‘Debreceni bőtermő’ is very similar to ‘Pandy279’ showed lowest (12%) fruit set. Seasonal changes of fruit set and drop shows that there are four abscission peaks. The first fruit abortion wave appears during second week after pollination Thereafter, the second and third dropping period was found during the third and forth weeks after pollination. The forth abscission happened on the forth week after pollination. The highest fruit drop happened on ‘Pandy279’ (92.4%) very similar to ‘Éva’ (90%) while the lowest fruit drop observed in ‘Oblocsinszka’ (71.5%)
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Association of varieties in stone fruit plantations
29-33.Views:166In the majority of Hungarian orchards of stone fruits, the planting distance is 6-7 m x 4-5 m. As many of the current varieties are self-incompatible, planting designs are applied to provide for adequate pollinisers. As long as differences in blooming time are small, i.e. 3-5 days at most, overlaps of blooming of the associated varieties are sufficient for fruit set.
In sour cherry, one leading variety, Pándy, is self-incompatible and requires two polliniser varieties at least (Ciganyneggy or some sweet cherry varieties). Pándy is, moreover, cross-incompatible with the varieties Debreceni bőtermő, Kántorjánosi and Újfehértói fürtös being all of them self-fertile as most of new varieties recommended, by the way, for being planted to monovarietal blocks.
Among European plums there are varieties registered as male sterile, self-incompatible, parially self-fertile and self-fertile, respectively. For the purpose of cross pollination, the choice of two varieties, at least, to be associated to any variety belonging to the first three groups, is recommended. The number of rows in blocks planted to self incompatible or male-sterile varieties should not be higher than 2-(4). Inter-incompatibility has been observed within the currently recommended assortment, between the varieties Cacanska najbolja and Stanley, only. Chinese-Japanese plums are scarcely represented in Hungarian plantations. Variation of blooming time in varieties is somewhat more pronounced, i.e. 5-8 days. There is but a weak tendency to self-fertility, thus practically, all varieties are considered as self-incompatible, thus the planting of two-row blocks for each of three varieties, at least, are recommended to be associated.
Self-incompatibility and partially self-fertile apricot varieties are recommended to be combined with two polliniser varieties, at least, each planted to two-row blocks. The varieties Ceglédi óriás, Ligeti óriás, Nagykőrösi óriás and Szegedi Mammut are mutually inter-incompatible. Most of the peach varieties grown in Hungary are self-fertile, thus they are planted to large blocks, each. On sites threatened by late spring frost, it is recommended to plant (monovarietal) blocks of 4-6 rows at most. Cross-pollination may increase fruit set even in self-fertile varieties.
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Biological performance of grape varieties in Eastern Hungary
31-35.Views:201Eurasian and interspecific grape varieties and candidates were inspected for biological productivity in the variety collection of the University of Debrecen at the Horticultural Experimental Station in Pallag. In this paper, data are reported on yields (kg/stock), cane production (kg/ stock) and use-up index of wood yield of each cultivar in the experimental years 2011-2012. From the range of Eurasian (Vitis vinifera L.) white wine-grape qualified cultivars and candidates ’Ezerfürtû’, ’Generosa’, ’Jubileum 75’, ’Müller Thurgau’, ’Zöld veltelíni’,’B-11’, ’CSFT-92’, ’Pintes’, from the range of Eurasian (Vitis vinifera L.) red wine-grape qualified cultivars and candidates ’Kármin’, ’Pinot noir’, ’Alicante Bouschet’ and ’Rubintos’ were highlighted. Promising data of interspecific ’Aletta’, ’Csillám’, ’Kunleány’, ’Orpheus’, ’Refrén’, ’Taurus’, ’Viktória gyöngye’, ’Alföld 100’, ’Reform’, ’RF38/32’, ’Toldi’ white, and ’Dunagyöngye’ ’Pannon frankos’ red wine-grape cultivars and candidates were emphasized. Published data refer only to the biological performance of the cultivars. The thorough evaluation of varieties needs respect to data on resistance to fungal disease and climatic extremities, and other characteristics concerning production technology and oenological parameters.
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Applied spatial analytic tools in orchards with special regard to agro-ecology
17-19.Views:204Our investigation was carried out in several orchards in Hungary. This study reviews applicability of the different spatial analytical techniques in orchard especially models based on surface relief, such as digital elevation model, digital terrain model, slope and aspect maps. In recent time, the generation of digital elevation model has become a popular examination method. However, the elevation models generated from contour lines or elevation points could be applied to evaluate agro- ecological potential of fruit orchards to some extent. The highest deficiency of these models is the fact that these show the altitude of soil surface only and do not demonstrate landmarks on the surface. Consequently, the calculation of the radiation value results data relating to soil surface. The terrain model generated and applied by our team demonstrates fine surface texture and the landmarks in the orchard, so it is suitable for further examination.
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The effect of pre-fruiting temperatures on the yield of Pleurotus strains
49-51.Views:151Authors studied the effect of different temperatures prior to fruit body appearance on the yield of 3 Pleurotus strains P70, HK35 and 357. After 17 days of incubation at 25 °C and 28 °C the substrate blocks were kept for 7 days at 13, 16, 19, 22 and 25 °C. Then temperature was reduces to 16 °C during harvest time. Tests showed difference of some degrees in the optimal prefruiting temperature requirements of the strains which could result in even in 10-15% surplus yield. It is advisable to use technologies specially adapted to strains.
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Mass and displacement relationships of tree shakers
36-40.Views:108The degree of mechanically harvested fruit removal by shakers is determined by the displacement pattern, the frequency and the amplitude of shaker mechanism attached to the tree trunk. The displacement pattern is derived from the structural setup of the vibration mechanism, the frequency can easily be adjusted by the rev/min (rpm) of the rotating masses. More problems are arising in determining the dynamics of the tree-shaker system. Among others the amplitude of the attachment point and its components, the effective masses reduced to the attachment point.
Recent work gives some new insight into the tree-shaker dynamics including new calculation methods to determine the displacement components and the effective masses reduced to the attachment point. A fully new approach is given to include the vibrating soil mass into the total effective mass. The assumptions are supported and verified by laboratory and field measurements.
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Grape rootstock - scion interactions on shoot growth and cane maturing
30-34.Views:170Interaction between the rootstock and scion varieties (clones) was examined. Berlandieri x Riparia T. K. 5BB, Berlandieri x Riparia T.5C being the most widely used varieties in Hungary, Fercal, Ruggeri 140 being recently used on special soil conditions ; Georgikon 28 a new established variety and Berlandieri x Riparia T. 8B GK 10 clone were the rootstocks used. The V. vinifera L. varieties were a clone of Italian Riesling and two new hybrids from the same cross population f ( Noble Italian Riesling X Ezerjó) X ( Noble Italian Riesling X Pinot Gris ) }. This study presents the results how the growth habit depends on different scion/stock combinations in the first three years.
Shoot growth characters were observed during the initial years after plantation in the vineyard until we got the first yields. Our results did not agree with those of Pospisilova (1977) that the anticipated vigour of shoot growth will appear later in the vineyard only. However we agree with Zimmerman (1970), who showed that the differences are caused by the rootstocks in the growth habit of vines during the first two years already. The differences in shoot elongation decreased similarly to the rootstocks in the first year of fruit production. It shows us that the intense root development caused vigorous shoot growth, which we think to be a rootstock effect. When the vegetative and generative development are balanced already, the effect of scions in shoot elongation became stronger than the effect of rootstock.
We also found great differences in the maturation of wood each year from planting of the vineyard to the year of fruit production.
During the first years the differences of cane maturation were greater according to the scion varieties, the means were significantly different. In the first ripening year the differences dwindled or increased due to the rootstocks. This means that the effect of the rootstock on cane maturation became stronger in the first year of fruit production.
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Genetic engineering of apple (Malus domestica Borkh.) for resistance to fungal diseases using g2ps1 gene from Gerbera hybrida (Asteraceae)
15-12.Views:266In the present study, g2ps1 gene from Gerbera hybrida coding for 2-pyrone synthase which contribute for fungal and insect resistance was used. The aim was to work out an efficient approach of genetic transformation for apple cvs. ‘Golden Delicious’, ‘Royal Gala’ and ‘MM111’, ‘M26’ rootstocks for improving their fungal resistance using genetic engineering techniques. Adventitious shoot formation from leaf pieces of apples studied was achieved using middle leaf segments taken from the youngest leaves from in vitro-grown plants.
Optimum conditions for ‚direct’ shoot organogenesis resulted in high regeneration efficiency of 0%, 95%, 92%, 94% in the studied apples respectively. Putative transgenic shoots could be obtained on MS media with B5 Vitamins, 5.0 mg l-1 BAP, or 2.0 mg l-1 TDZ with 0.2 mg l-1 NAA in the presence of the selection agent “PPT” at 3.0-5.0 mgl-1. Shoot multiplication of transgenic shoots was achieved on: MS + B5 vitamins + 1.0 mg l-1 BAP + 0.3 mg l-1 IBA, 0.2 mg l-1 GA3+1.0 g/l MES+ 30 g/l sucrose + 7.0 g/l Agar, with the selection agent PPT at 5.0 mg l-1 and were subcultured every 4 weeks in order to get sufficient material to confirm transformation of the putative shoots obtained. Six, seven, one and six transgenic clones of the apples studied respectively have been obtained and confirmed by selection on the media containing the selection agent “PPT” and by PCR analysis using the suitable primers in all clones obtained for the presence of the selection” bar gene (447 bp) and the gene-of- interest “g2PS1” (1244 bp), with transformation efficiency of 0.4%, 0.6%, 0.1% and 0.3% respectively. These transgenic clones were multiplied further in vitro in the presence of the selection agent ‘PPT’ and rooted in vitro. Rooted transgenic plantlets were successfully acclimatized and are being kept under-containment conditions according to the biosafety by-law in Syria to evaluate their performance for fungal resistance .