Search
Search Results
-
Comparative investigation on Hypericum perforatum L. populations of different origin
56-60.Views:174Widespread application for herbal medicines based on Hyperici herba has been experienced in the last few years, especially in the treatment of depression syndrome. As the wild origins could not satisfy the market demands neither in quantitative nor in qualitative respect, the necessity of the development of intensive growing methods has been raised. In the course of our investigations we intended to clear up the morphological and chemical variability among and within populations of different origin in order to start a new breeding program. According to our data, growth dynamics of populations could be characterised by a logistic curve. According to the time of flowering the populations formed early, middle and late groups. Morphological diversity among populations was measurable in differences of flower length, plant height, different leaf types and plant habit, according to which characteristics groups were distinguished. Generally, the accessions were the most homogenous in plant height (CV: 7-15%), followed by inflorescence-lengths (CV: 11-36%) and the least uniform characteristics proved to be the number of flowering shoots (CV: 14-59%). The greatest morphological heterogeneity was experienced in the accessions of wild origin as it has been expected. In the second vegetation period generally a much better homogeneity was obtained, than in the first year. The most outstanding accessions produced 1.2 t of dry flowers and 4 t of dry flowering shoots, calculating to one hectare area. The individual yields varied on a large scale in each population (CV: 18-70%).
The content of hypericin varied between 1,85 and 9,9 mg/g in 1996, and between 0,18 and 2,70 mg/g in 1997, showing high individual divergences. Flavonoid values -including first of all hyperosid, rutin and quercitrin - reached 17-39 mg/g in the first and 15-20 mg/g in the second year, respectively. Individual and seasonal variation was less than in hypericin. A joint high level of these two compound groups seems to have low frequencies.
The superior population were selected for further breeding.
-
Evaluation of generative accomplishment of new apple cultivars in Hungary
11-15Views:251In a two year study, 14 new apple cultivars (cvs ’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’ and club cultivar) were studied in Eastern Hungary at Nyírbátor. The following parameters were measured: trunk cross sectional area (cm2), harvest time with yield (date, kg/tree, number/tree), fruit size (mm), fruit surface color (%), and color intensity of the fruit surface (1-5). According to our two year assessments regarding, the best generative accomplishment was achieved for the cvs ‘Gala’ sorts trained to slender spindle and the cv ‘Red Jonaprince’ trained to super spindle. According to the trunk cross sectional area the ‘Gala’ sport trained to slender spindle showed the strongest growth among the 4 years old trees, while the cv ‘Fuji SW’ and the cv ‘Jeromine’ presented lower vegetative accomplishment. The ripening time of the cultivars started at the end of August and finished at the beginning of October. ‘Gala’ mutants trained to slender spindle provided the highest yields. Fruit size of all cultivars reached the market required standard, however, cvs ‘Red Jonaprince’ and ‘Red Idared’ produced large fruits over 90 mm. The fruit size of the ‘Gala’ sort was 72–75 mm. Most of the cultivars showed high fruit surface color and color intensity.
-
Variety specific integrated fruit production development in order to optimize inner content values
27-31.Views:168In this paper we introduce our results of three years (2007-2009) investigatio ns carried out in the framework of "Research and development in foodstaff chain" - project of Regional University Knowledge Center by the members of Corvi nus U niversity of Budapest - Department of Pomology and. Research Institute for Fru it growing and Ornamentals. Budapest-Erd. The main objectives of the project were the followings: submi ssion of sour cherry candidate suitable for industrial process for state approval; determination of physica l parameter and inner content value changes of sour cherry varieties duri ng ripening; evaluation of health care attributes of sour cherry fruit; ripen ing process description by the colour and the force required to pick fruits of sour cherry. We stated that the optimal begi nning and period of the harvest can be determined wi th the fruit removal force. In this period the fruit growth stops. ju iciness rat io does not change. refractions are approximately equal.acidic content turns to decreasing trend.However the proportion of anthocyan in and polypheno l can still increase. Fruits harvested in this period fulfil a wide range of industrial process opportunities. 'Erdi jubileum ·and candidate IV-3/48 according to their inner content values arc suitable for high quality products (containing real fruit material in high proportion).
-
Tomato (Lycopersicon esculentum Mill.) anatomical, physiological, biochemical and production responses to drought stress - A mini-review essay
40-45.Views:782The cultivated plant climate association in agricultural yields is getting expanded consideration with regards to changing climatic conditions. Abiotic stressors can lead to morpho-anatomical, physiological, and biochemical alterations in harvests, resulting in a significant loss of profit. A comprehension of ecological elements and their communication with physiological cycles is critical for improving agricultural practices. Drought stress is among the main natural factor affecting plant development, growth, and yield measures. Assessing the impact of environmental change and atmospheric variability on tomato crop output will require a thorough understanding of this stress element. The physiology, development, improvement, yield, and quality of the tomato crop are all affected by dry season stress. This mini-review essay presents the most prominent features about the effects of drought stress on tomato crop plant physiology and production, with specific highlighting for the complex relationship between drought stress, and nutrients uptake.
-
Real-case application of mycorrhizal inoculums on Capsicum annuum L. var. longum cv. Szegedi and Kalocsai
75-79.Views:175The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, a mixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF) was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights of shoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weights due to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative crop production compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai. Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsai and Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonization was observed in June in general and colonization percent increased during vegetative development and there was a slight decrease at harvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spice pepper production.
-
Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA
101-104.Views:127Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-life has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.
-
Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:183A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
-
Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
75-79.Views:111Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.
-
The inheritance and durability of scab resistance in apple progenies
39-46.Views:128In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
-
Investigation of the in vitro regeneration of mericlones in the caribe variety of carnation
87-89.Views:129In vitro culture conditions were experimented for the relatively sensitive, but very esthaetic "Caribe" variety of carnation with uniformly dark violet flowers. Regeneration of new plants from shoot apex meristems can be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. Callus formation as a prerequisite for the induction of somaclonal variability can be achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Acclimation of the obtained mericlones to the ex vitro conditions was also evaluated.
-
Vegetative parameters of sweet cherry (Cerasus avium L.) cultivars in two training systems
42-45.Views:256Hungarian sweet cherry production has been changed recently. Thanks to the favourable selling prices many new plantations were established in the last years. In the new orchards mainly spindle canopy are trained, although many type of crown forms are used all over the world. In our study slender spindle and bush canopy were evaluated with three cultivars (‘Petrus’, ‘Vera’, ‘Carmen’) grafted on Prunus mahaleb rootstock. Vigor of the 3-4 years old trees showed spectacular differences. The highest vigor with Spanish bush was found for cv. ‘Vera’, but on slender spindle canopy the thickness values were medium. Meanwhile cv. ‘Petrus’ showed the highest trunk thickness with spindle canopy, but the lowest with Spanish bush. On spindle canopy cv. ‘Carmen’ presented the highest ramification ability, but its 2-3 years old twigs started to bald very early, which can be considered an unfavorable phenomenon. Bush canopy still can be described with strong vegetative growth on 3-4 years old trees, as vigor of spindle trees are more moderate.
-
Pruning of plum trees and maintenance of balanced yielding
131-138.Views:204In this review, we give an overview of the pruning techniques that should be applied in commercial plum orchards. First, a detailed description of pruning for shaping the different crown types for the first, second, third, fourth and fifth years and the phytotechnical works is given for crowns with a circular projection area. Then, pruning for open crowns forms, such as the vase form is discussed. Following the shaping of the crown, it should be maintained by pruning. Detailed information is given on pruning for maintenance with special emphasis on the comparison of winter and summer pruning. In the following part of the manuscript, pruning experience is described separately for the major commercial plum cultivars in Hungary such as Cacanska lepotica, President, Bluefre, Stanley. Recommendations for cultivation systems under non-irrigated conditions are also included. Finally, light requirements, growth characteristics and suggested crown forms of the different cultivars are described for cvs. Ageni, Althan Tingle), Besztercei szilva Nm. 122, Bluefre, Cacanska lepotica, Cacanska rodna, Debreceni muskotály, Montfort, President, Ruth Gerstetter, Sermina, Stanley, Tuleu gras and Zöld ringló).
-
Factors affecting rhizogenesis in vitro and acclimatisation of three cherry rootstocks
40-46.Views:127Factors affecting rhizogenesis in vitro and acclimatisation of three rootstocks of cherry, i.e. Mahaleb, Maxma-14 and Weiroot -10 were investigated.
Rooting was easily achieved within 2-4 weeks on MS-based liquid or agar-gelled media containing auxins IBA at conc. 0.49 or 2.45 pM or NAA at conc. of 0.49 pM. On liquid media with 2.45 pM IBA, a maximum rooting efficiency of 95-100% was obtained. However, high concentrations of auxin delayed the time of root initiation for 3-5 days.
Rooted plantlets were transplanted into pots with a mixture of 3:1 (v/v) peat:perlite and acclimatised gradually to field conditions with efficiency of 60%.
-
Review on rain induced fruit cracking of sweet cherries (Prunus avium L.), its causes and the possibilities of prevention
27-35.Views:725The rain induced fruit cracking is a big, serious and costly problem for cherry growers. Cracked fruits lose their value and they are not marketable because of the poor fruit quality. Cracked fruits have different storage diseases and shorter storage and shelf life.
There are many influencing factors of the cherry fruit cracking such as: water uptake; fruit characteristics (fruit size, fruit firmness; anatomy and strength of the fruit skin, stomata in fruit skin, cuticular properties, osmotic concentration, water capacity of the fruit pulp, growth stage of the fruit,); orchard temperature and other environmental conditions;
The most effective protection technique is the plastic rain cover over the tree rows. The installation of these equipments is too expensive for the cherry growers. That is the reason why researchers tried to find other less expensive and sufficiently effective ways against the Lim induced fruit cracking.
Several calcium formulas: calcium chloride (CaC1,), calcium hydroxide (Ca (OH)2) and calcium nitrate (Ca(NO3)2 ) can be sprayed in appropriate concentration. Spraying with other mineral salts (aluminium and copper salts, borax) and PBRs (Plant Bioregulators) may be also effective to reduce fruit cracking.
-
Nutrient demand of stone fruits
19-23.Views:134Effects of nitrogen, phosphorus and potassium fertilization were investigated on the change of nutrient content, vegetative and generative production of apricot, peach and sour cherry trees, as well as on frost hardiness in long term experiments. Nitrogen and phosphorus fertilization increased only the concentration of these elements in cherry leaves without effect on growth and yield. Consequent potassium effect was proved on these stone fruit species. Effect on yield appeared following the first higher crop load.
Potassium supply has positive effect on frost hardiness of apricot and sour cherry flowers and peach flower buds.
In peach, the lime content of soil decreased the yield but it could be compensated by potassium dressing to some extent. Favourable nutrient boundary values were determined for soil and foliage.
-
Role of hydrogen peroxide and Pharmaplant-turbo against cucumber powdery mildew fungus under organic and inorganic production
39-44.Views:410Cucumber leaves have been sprayed with a solution of hydrogen peroxide (H202) or Pharmaplant-turbo combined with organic or inorganic fertilizers under plasic house. Under the influence of H202, leaves exhibited resistant against Podoshaera fusca fungus, the causal agent of cucumber powdery mildew. H202 (15 mM) was able to decrease the disease severity from 90.4% to 12% in two experiments conducted in two seasons. Pharmaplant-turbo (Turbo) is new chemical compound and used as an antifungal compound. Turbo in 1 ml/L was able to decrease the disease severity from 90.4% to 11.5% in the both experiments as well. Both of H202 and Turbo were combined with organic treatment (compost + compost tea + seaweed extracts) which showed significant effect against cucumber powdery mildew fungus and strongly suppressed it as compared to control leaves. Organic treatment produced higher vegetative growth characters and greater early and total yields as compared to inorganic treatment, also organic fruits produced the lower nitrate content and the higher ascorbic acid content as compared to inorganic fruits. Our study have indicated that, H202 and Turbo combined with organic fertilizers play a role in the resistance of cucumber against powdery mildew by decreasing the disease severity. We suggest to give more attention to the direct application of H202 in low concentration and Turbo against powdery mildew diseases and other plant diseases.
-
Bee pollination and association of apricot varieties
20-24.Views:188Apricot yields are highly variable according to the season. The variation is caused mainly by the adversities during the critical processes of floral biology, i.e. blooming and fertilisation. On the basis of information concerning blooming time and mutual compatibility relations of apricot varieties a system of securing regular and adequate yields has been developed.
Winter frosts of the continental type are well tolerated by most of the apricots, however, after the end of rest period, flower buds are loosing frost tolerance, 'rapidly.
Being one of the fruit species blooming earliest during the early spring, apricot start to bloom in Hungary around the end of March or early April as a mean of many years, but it also happened, exceptionally that apricot started to bloom at February 20 (at Letenye South Hungary). The early season, exposes the floral organs to frost injuries. As a consequence, apricot orchards on the Great Plain produce low yields in 3 years, intermediate yields in other 3 years out of a ten-year-period.
Moreover, weather conditions during the blooming period are often unfavourable for pollination. Cool, windy and rainy weather prevents the flight of insects and on the other hand, warm spells shorten the blooming process, nectarines and stigmata get dry and the female gametes loose viability before effective pollination occurres.
The fertility of individual cultivars are meeting different obstacles. Apricot cultivars differ greatly in the rate of flowers bearing underdeveloped pistils, which may attain even 60% (e.g. Orangered). New commercial cultivars are often self-incompatible. Local varieties of that type in Hungary are the „óriás" varieties (e.g. Ceglédi óriás, Szegedi mammut), and the new hybrid Ceglédi Piroska. Many of the cultivars are variable in their self-fertility (partially self-fertile): Budapest, Harmat, Korai piros, Mandulakajszi.
Inter-incompatibility is also known in apricots. The „óriás " varieties do not fertilise each other. During the growth of fruits, cool spells (2-4 °C) caused severe fruit shed in Ceglédi óriás.
Apricot flowers produce pollen and nectar at average rates related to other fruit species, thus bees are attracted sufficiently. Bee visits are very variable according to growing site and season. Most of the bees are pollen gatherers but sometimes nectar suckers are in majority. Bee pollination is necessary not only for the self-incompatible varieties but also to enhance the yield of self-fertile varieties.
Taking the blooming and fertility relations of the cultivars into account, plantations should not exceed two rows to a particular self-incompatible varieties, and possibly two different polliniser varieties are suggested to be planted as flanking the block in question.
In commercial plantations 2 to 4 bee colonies per hectare are proposed to move for the whole blooming period.
-
Estimating of water consumption of cherry trees
15-17.Views:175Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday- I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.
-
New sweet cherry cultivars in intensive plantings
13-16.Views:312The study took place in the largest sweet cherry plantation in West Hungary. The purpose has been the identification of those varieties, which will be suitable for intense cultivation, early fruiting and excellent fruit quality, moreover, the selection of the optimal phytotechnical procedures. At the same time, scion-rootstock combinations have been tested also from the point of view of growing intensity and fruiting in high-density plantation. The dense planting is induced to start fruiting early and yield regularly by special methods.Yielding was stimulated by maintaining the balance of vegetative-generative growth by binding the shoots, by summer pruning, by cuts on the trunk and root pruning. Best experiences have been found in yield and quality in the following varieties: Canada Giant, Carmen, Firm Red, Giant Red, Katalin, Kordia, Regina. Dense planting has been feasible also on vigorous rootstock, like P. mahaleb. Dwarfing rootstocks like P-HL-A, Gisela 6, accelerate the formation of flower buds and yielding earlier with fruits of adequate size. ‘Firm Red’ and ‘Giant Red’ excelled with their large fruit (>27 mm diameter) in all combinations, thus being promising under Hungarian conditions.
-
Evaluation of hot pepper (Capsicum annum L.) varieties for green pod yield and quality under rain fed production at Teppi, South Western Ethiopia
45-49.Views:199The trials were designed with three replications in a randomized complete block design in order to evaluate the phenological, growth and yield potential of hot pepper varieties such as ‘Mareko fana’, ‘Melka Zala’, ‘Melka Awaze’, ‘Melka Shote’ and local check. Significant difference was observed between growing year and used varieties. The varieties also performed significantly different (p<0.05) for most of the considered traits in the study. The result revealed that varieties ‘Melka Zala’, ‘Mareko fana’ and ‘Melka Awaze’ were scored highest green pod yield of 8.39, 8.71 and 11.39 ton per hectare, respectively. However, ‘Mareko fana’ variety was susceptible to disease attack as compared to other varieties. Therefore, promoting both ‘Melka Awaze’ and ‘Melka Zala’ varieties for widespread production for Teppi and the areas with similar agro-ecological conditions could contribute to boost the productivity of hot pepper. ‘Mareko fana’ could also be used for dry pod purpose due to its attractive color.
-
Sunburn incidence of apples is affected by rootstocks and fruit position within the canopy but not by fruit position on the cluster
45-51.Views:220Authors investigated sunburn incidence of apples on the combinations of three different growth inducing rootstocks (M.9,MM.106 and seedling) and five varieties (‘Smoothee’, ‘Golden Reinders’, ‘Granny Smith’, ‘Gloster’ and ‘Jonagold Jonica’). Symptoms were classified as sunburn browning, sunburn necrosis and photooxidative sunburn. The frequency of symptoms was recorded at various parts of the canopy (N, E,W, S, and lower canopy, upper canopy) and on the cluster (terminal, lateral). Cultivar susceptibility varied between 0.30 and 5.65% on M.9 rootstock, ‘Granny Smith’ seemed to be the most susceptible cultivar whereas relatively low percentage of damaged fruit was observed for ‘Gloster’. On MM.106 and seedling rootstocks, damage level was significantly lower than on M.9. Remarkable differences were not observed in the share of the three sunburn types between cultivars. The most common symptom observed was sunburn browning. Far less fruit was affected by sunburn necrosis and photooxidative sunburn. Photooxidative sunburn symptoms were not found on ‘Granny Smith’ and ‘Gloster’ fruits on MM.106 rootstock. Latter cultivar did not show sunburn necrosis symptoms either. With increasing growing vigor of the rootstocks the share of sunburn browning increased. Fruits with sunburn symptoms were found in a great majority on theW quadrant of the trees. This was true for all cultivars. Remarkable differences in the location within the canopy of affected fruits between the three types of sunburn were not observed. Specific distribution of sunburned fruit was observed along the vertical axis of the canopy, too. Most of the damaged fruit were found in the upper canopy. This is particularly true for trees on vigorous stocks such as MM.106 and seedling. On M.9 rootstock, depending on cultivars 5.9 to 38.9% of sunburned fruit was located in the lower canopy. Most common symptom in the lower canopy was the sunburn browning, however symptoms of sunburn necrosis were not found at lower canopy level. Low rate of photooxidative sunburn was observed such lower canopy conditions. Sunburn incidence was very similar on king or side fruit. Significant differences were not found in the share of each sunburn types between fruit positions on the cluster. This was not influenced by rootstocks either.
-
Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation
30-38.Views:311Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.
-
Carbohydrate utilization of Erwinia amylovora in vitro
31-34.Views:152Nectar is a multi-component aqueous solution that promotes bacterial multiplication. The concentration of nectar in plant flowers is not stable since it is under the influence of environmental conditions, especially free moisture and relative humidity. Experiments were conducted with "artificial nectar" and directed along two lines: (1) determination of the optimal concentrations of carbohydrates for the growth of E. amylovora development (2) consumption of different carbohydrates besides basic sugars.
Solutions of "artificial nectar" were prepared in different compositions by changing the dominance of basic sugars (fructose — glucose —sucrose) in proportions of 2:1:1, 1:2:1, 1:1:2 and between concentrations of 10-0.6% (diluted with Basal minimum broth) in order to determine optimal conditions for the development of E. amylovora.
At a basic sugar concentration of 10% bacterial multiplication started and continued until I log degree (from 106 to 107 cfu/ml). At concentrations of 5% and 2,5 % cells developed with nearly the same kinetics (from 106 to 8x107 cfu/ml and from 106 to 9x107 cfu/ml, respectively). Multiplication was more pronounced and nearly the same at concentrations of 1.2 % and 0.6 % (from106 to 2x108 cfu/ml). At a basic sugar concentration 30% total sugars bacterial multiplication did not occur, while at 20 % it was negligible, not measurable photometrically.
At minimal concentrations of F, G, S (between 1-0.1 %) bacterial cells were still able to multiply, producing organic acids from sugars.
Our study showed that E. amylovora requires only a small amount of sugars (0.1%) for multiplication (acid production) while high concentrations inhibit multiplication. There was a negative correlation between sugar content and cell density. The optimal range of sugar concentration was at about 1%.
Effect of "less frequent carbohydrates" to E. amylovora multiplication was also determined using the API 50 CH strip. We could provide information on utilization of 39 carbohydrates by the bacterium at different categories as follows: Not utilized-, Slowly and weakly utilized-, Slowly and completely utilized-, Quickly and completely utilized carbohydrates. We suppose that carbohydrates that belong to the latter two groups could play an important role as nectar components in promoting E. amylovora multiplication in the blossoms of pome fruit trees.
-
Sustainable food production with greenhouse technologies
99-105.Views:194The greenhouse fruit and vegetable production is one important area which supports sustainability. To achieve
sustainable economy and growth, the given natural resources have to be used in a smarter, renewable way in order to avoid
depleting them. New technologies and new methods are developed and implemented to utilize resources in more optimized way.
Sustainable food supply is essential globally for the world, however it has to be managed and achieved on local levels. We present
the greenhouse production market restructuring with new players. What kind of difficulties arise in the open field vegetable
production and what benefits can be realized by the customers and producers from the covered greenhouse technology in the
continental and desert climate? What are the technical boundary conditions to establish and operate greenhouse production in
different regions and what are the benefits realized from local food production? As an example, we analyse Qatar’s energetics,
climate conditions and food resources, Qatar’s food supply process and its barriers. We will show how the sustainability and
food safety appear in Qatar’s National Strategy Plan. -
A study of processes active in regeneration of different sweet cherry varieties
55-57.Views:206Cherries belong to the group of fruit trees, which require little attention in pruning. The process of getting bald branches is less accentuated, which is associated with the light requirement of organs changing along with the aging process. The growing habits of the varieties (length and number of growing shoots, etc.) is closely related with the productivity of fruiting parts. The ability of regeneration and the functional effectiveness of fruiting structures are expressed by the distribution according to their age. The differences registered justify the necessity to develop pruning policies for each particular type of variety. For that purpose, we have to examine the age dependency of the processes related to regeneration, which may serve as starting point of a pruning strategy. Growth of some parts should be stimulated in order to develop or regenerate fruiting parts. Some branches need to be rejuvenated, partially, as an antidote to progressive senescence. Every intervention is aimed to strengthen the development of the most productive constructions. Reactions induced by rejuvenation should be expressed in fruiting intensity. A pruning program adapted to the particular variety as well as to the actual phase of a rotating pruning system should anticipate results.