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Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation
53-56.Views:137The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.
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Ecological drought resistance and adaptability of apple varieties
113-122.Views:295For adequate yields in apple plantations, during the long growing period of the fruit primordia, one of the decisive factors is water supply. Indicators of stress are valuable signs for the diagnosis of drought and necessity of watering, i.e. planning the irrigation of plantations. The aim of the present study was to find reliable signs of water stress on apple trees and at the same time conclude on the drought tolerance of different varieties. The plantation of apple varieties grafted on various stocks and cultivated according to different systems (irrigated, non irrigated, integrate and biological) has been examined continuously by leaf analysis. Along the period of growing fruits, measurements were made in the field, then the leaf samples were analysed in the laboratory for composition of pigments, carbohydrates and antioxidants, as well as the histology of the tissues checked. Without irrigation, the mean leaf mass and the relative chlorophyll content (SPAD) of the variety Idared on M4 stocks increases beyond the values of 50 SPAD, whereas on M26 stock and integrated system, it declines. In the collection of varieties, grown according to the „integrated” system, during the development of fruits, ‘Gála’ and ‘Remo’ varieties have been affiliated to the “less susceptible” group regarding drought tolerance because of the leaf morphology, structure and content of SPAD and antioxidants. However, ‘Idared’ and ‘Jonagold’ belong to the “susceptible” group. To the same group are ranged the ‘Akane, Red Rome vanWell, Pink Lady’ varieties. Those varieties reacted to a short period of drought by increased production (content) of carbohydrates. Regarding changes of carbohydrate content ‘Greensleeves’ and ‘Ozark Gold’ varieties belong to the moderately water dependent (requiring) group. In the field, SPAD-tests facilitated the measurement of nutrient-uptake and incorporation, which is proved by the tight correlation between the data of SPAD and the increment of leaf weight (r=0.76–r=0.88), however, this depends on the variety too. SPAD is an indicator of water supply and is related with the density of stomata, cannot used for the selection of water-exigent varieties but for (drought) tolerant ones. In integrated culture, the (drought) susceptible varieties display (water-soluble) hydrophilic and lipophilic antioxidants (ACWand ACL resp.) the quantity of which may have some role in drought tolerance.
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Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:354In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
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Virus susceptibility and resistance of Hungarian pepper varieties
68-73.Views:156The aim of our study was to examine susceptibility or resistance of 18 pepper varieties to four viruses [tobacco mosaic
tobamovirus (TMV), sowbane mosaic sobemovirus (SoMV), NTN strain of potato Y potyvirus (PVYNTN) and cucumber mosaic cucumovirus (CMV). Out of the 18 varieties, 13 were resistant to CMV infection. Thirteen varieties were susceptible to TMV, while five ones (Dabora Fl, Brill Fl, Feherozon Synthetic, Ciklon Fl, Cecil Fl) showed only local hypersensitive reaction. All of the tested pepper varieties showed resistance to SoMV. Eight varieties (Tuba, Fehérözön Synthetic, Boni, Alba Regia, Korona, Édesalma, Cecil Fl, Star) were found to be resistant to PVYNTN. Out of the examined varieties five (Boni, Alba Regia, Korona, Édesalma, Star) were resistant to three viruses (SoMV, CMV and PVYNTN). Only one (Cecil F1) displayed complex, extreme resistance to SoMV, PVYNTN, CMV and hypersensitive reaction to TMV, therefore this hybrid is very important in pepper breeding and growing for virus resistance.
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Micropropagation of Rudbeckia hirta L. from seedling explants
105-108Views:162We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strenght solid Murashige and Skoog (1962) culture medium containing 10 mg/1 of kinetin or 2 mg/I of kinetin + 0.1 mg/1 of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/1 of IAA. After four weeks of incubation we obtained elongated shoots which we separated and inoculated into a new culture medium and we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respect of breeding.
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The fertilization problems of cultivated red- and black currant varieties in Hungary
38-40.Views:119Small fruits have a modest share in the fruit production of Hungarys. Red currant was grown traditionally in home gardens 60-70 years ago. Commercial production was established only in the surroundings of some town. The black currant was unknown until after Wold War II. An important change occured in small fruit production in the 1950s. Socialist countries, which had cheaper labour power, made efforts to meet these demands. In this time we produced 25.000 t.
Presently the country produces 13-15.000 tons currant fruit yearly 60% from this is black currant, which has a better market. It is our own interest to make our currant production more profitable. The currant is the second most widely cultivated soft fruit. Our product is disposed mostly on EU markets.
There was no breeding activity in this field in Hungary earlier. Cultivars used were mostly of foreign origin (W. European; Boskoop Giant, Silvergieter, Wellington XXX, Russian; Altaiskaya Desertnaya, Neosupaiuschaiasya, N. European; Brikltorp, Ojebyn). Besides well-known advantageous this cultivars have also some defects mainly unfavourable—adaptation to climatic conditions, which caused fertilisation problems, reduced the fruit set and uneven growth with decreased yields (Dénes & Porpáczy, 1999). About 140 black currant cultivars were investigated during the last four decades in our variety trials and only four of them were introduced with satisfying yielding capacity (3.5-5.5 t/ha).
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Preliminary results of fruit quality of resistant sour cherry clones in 2014
31-34.Views:156The richness of Hungarian sour cherry cultivars in the world is unique; they can be consumed in many ways. Sour cherry is a Hungaricum and has excellent fruit quality and nutritional value. Nowadays the demand for chemical-free fruits is increasing. Reducing the number of chemical applications can be achieved most effectively by the use of resistant varieties, technological developments and cultivar innovation. The domestic and international sour cherry breeding programs use almost exclusively the resistant ‘Csengôdi’ clones as a basis. They are mainly Monilinia and Blumeriella resistant and have higher dry matter and antioxidant content. These pathogens influence not only the qualitative and quantitative parameters of the fruits, but may play an indirect role in the deterioration of the overall condition of the trees as well. The cultivation of resistant varieties can greatly reduce the pesticide load of the environment, so we can produce fruit with excellent nutritional value with fewer chemicals. Our aim is the expansion of the sour cherry assortment by introducing new resistant cultivars. With this we can contribute to the spreading of environmentally sound production technologies and supply “super fruits” to the consumers.
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A complex system for the production of pathogen-free grapevine propagating material
59-62.Views:207The use of pathogen-free planting stock for new vineyard establishment is a key component in the maintenance and expansion of vine and quality table grape production. The success of the necessary changes in the structure of the grape industry is forced by the globalization process, the climate change, the rediscovery of autochton varieties as well as breeding of new tolerant and resistant varieties. The renewal of vineyards largely depend on the availability of planting stocks. Serbia and Hungary found a common interest in establishing pathogen-free stock materials from newly breed resistant varieties and clonal selections of varieties which are traditional in the Serbian-Hungarian border area. During a cross-border cooperation program a complex system for the production of pathogen-free grapevine propagating material was established. Using heat therapy, in vitro shoot tip culture and traditional and molecular diagnostic techniques new pathogen-free stock materials were established from 26 varieties. They have been or will be tested for the presence of most important grapevine viruses, phytoplasmas, as well as bacterial and fungal pathogens. The complex system applying green grafting for indexing on grapevine indicators can shorten the duration of the procedure from 4 years to two-three years.
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Effects of different pollination treatments in genotypes of Prunus salicina Lindl.
141-146.Views:118The low productivity in the Japanese plum (Prunus salicina Lindl) is related with self-incompatibility characteristics, so other species or varieties that act as pollinators need to be present to improve fruit production. The objective of this work was to study the efficiency of pollination in different genotypes of P. salicina using treatments of natural self-pollination, cross-pollination with P. armeniaca cv. Giada and open pollination. These treatments were evaluated through viability techniques and in vitro and in vivo germination of pollen grains; the growth of pollen tubes along the pistil was also observed. Genotypes used in this study showed differences for each one of the pollination treatments. Some genotypes showed signs of self-sterility and interincompatibility with P. armeniaca cv. Giada, while others showed partial self-fertility characteristics or pseudocompatibility. Moreover, some genotypes showed a higher affinity coefficient with cv. Giada and these will be indicating a possible intercompatibility. These studies will be an important contribution breeding and selection of intra and intercompatible genotypes to be used in commercial orchards.
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Susceptibility of some traditional pear cultivars of Hungarian and foreign origin to the pathogenic bacterium Erwinia amylovora
41-45.Views:137Fire blight, a disease caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., has been causing serious damage in Hungarian pear plantations since 1996. A prospective control measure could be the use of resistant cultivars. For that purpose ten pear cultivars have been tested under laboratory and greenhouse conditions for resistance to Erwinia amylovora strains collected in Hungary. Six of these cultivars are Hungarian ones of unknown origin, while four are traditional old varieties. Resistant cultivars should serve as germplasm for future breeding. Inoculations were made with a mixture of different pear isolates of the bacteria collected from various growing regions of Hungary (Ea 21, 23), at a density of 5x108 cells/ml. Susceptibility/resistance has been assessed on the basis of intensity of blight symptoms observed on shoots, flower parts and fruits. Cultivars were assigned to three susceptibility groups (low, moderate and high). Complete resistance was not found among the cultivars tested. The highest level of resistance was found in cultivar 'Kieffer', while the other cultivars displayed either moderate or high susceptibility to infection.
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Variability and differences of growth vigour in the set of 36 genotypes of apricot (Prunus armeniaca L.).
30-34.Views:121Growth vigour of 36 apricot cultivars and new hybrids grafted on apricot seedling rootstock (Prunus armeniaca L.) was evaluated on the base of measurements of stem girth from the 411' to the 10th year after planting. There were differences in growth vigour of genotypes under study. In the evaluated set of genotypes the control cultivar 'Veecor may be classified as a genotype with below-average growth vigour. Only four genotypes (-Reale d'Imola-, Sanagian -Moldavskii krupnoplodnyl and 'LE-2385') were found with significantly higher growth vigour than that of control cultivar 'Veecot' in years of the end of experimental period. Two genotypes (Farmingdale', -LE-SE0-24') were found with significantly higher growth vigour only at the beginning of experimental period and one cultivar ('Vivagold-) with significantly lower growth vigour in the first four years. Genotypes with different growth vigour can be used in further breeding programmes and/or as components inhibiting or supporting the growth in indirect vegetative propagation. Within the whole experimental period, the rank of growth vigour of genotypes practically did not change. This was demonstrated by highly significant or significant coefficients of correlation existing between individual pairs of years (r=0.32+ to r=0.96++). As far as the time difference between years in individual pairs of years was higher, the correlation coefficients were lower. In individual years, variability of growth vigour was relatively low and ranged from 9.83 to 13.64%.
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The inheritance and durability of scab resistance in apple progenies
39-46.Views:128In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
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Evaluation of some Achillea L. accessions based on morphological, cytological and chemical characteristics
69-74.Views:152Taxonomical evaluation of six taxa of the Achillea aggregate was carried out to determine their potential for breeding and cultivation. We used complex morphological, cytological and chemical aspects of characterisation. Three taxa of tetraploid genom and high chamazulene contents (33-40% ess.oil) proved to be A. collina Becker. A strain of wild origin had also high -bisabolol content and high oil level, comparable with the selected cultivars. A population was identified as new chemotype of A. pannonica Scheele with typical morphological and cytological traits, a wide sesquiterpene spectrum but lacking of 1.8 cineole and -pinene. We found a mixed population which consisted of both A. collina and A. pannonica plants. A tetraploid, azuleneless taxon could be concluded as A. pratensis Saukel and Langer not described before in Hungary. The diverse performance of the populations calls the attention to the significance of controlled plant raw in phytopharmaceutical products.
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Inheritance of male sterility in apricot
12-14.Views:128Progenies (total of 1,114 seedlings) from crosses representing all possible genotypic combinations between 4 male-fertile and 1 male-sterile apricot parents were scored for the male sterility trait. Crosses between putative heterozygous normal cultivars yielded 25% of male-sterile seedlings, which supports a previous hypothesis that male sterility is controlled by a recessive allele of one nuclear locus. Crosses between those parents and putative homozygous normal cultivars did not produce any male-sterile tree. Finally, the proportion of male-sterile progeny in crosses between a male-sterile and two male-fertile cultivars depended on the genotype of the male parent. When it was heterozygous approximately 50% of the progeny was sterile, whereas when a homozygous fertile parent was used, no male-sterile progeny was obtained. These results confirm a previously proposed model, in which the male sterility trait in apricot is controlled by a single recessive gene.
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Study of genetic resources for Powdery mildew resistance by biochemical and physiological parameters on peaches and nectarines in Romania
57-61.Views:122During 1997-2002, studies on the physiological and biochemical changes due to powdery mildew attack in peach and nectarine cultivars and hybrids belonging to different classes of resistance had been performed at the Research Station for Fruit Tree Growing (RSFTG) Baneasa Bucharest, located in the southern part of Romania. The study was based on disease assessments in the field and on other diagnosis criteria.
The southern part of Romania has favourable ecological conditions for growing peach and nectarine but trees have been attacked by various pathogens such as Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. persicae Woronichin fungus for a long time. The presence of the pathogen causes important changes in the quantity and quality of fruits and affects the metabolism of trees.
This study was focused on several physiological and biochemical parameters such as photosynthesis and respiration intensity along with catalase activity; and changes in some biochemical compounds such as free, total and linked water content, dry matter, nitrogen and protein content, tannin levels. The aim was to find possible correlation among the above parameters and the biotic stress induced by the powdery mildew fungus calculated as attack degree (AD%).
Results showed that photosynthesis diminished significantly in the case of the attacked phenotypes, the respiration was more intense in the infected cultivars and catalase proved to be less intense in the affected plants. The total water content significantly increased in contrast to the dry matter and tannin contents, which greatly reduced in the case of resistant cultivars such as 'Victoria', Vectared 7', 'KB 11-40'. The total nitrogen and protein levels showed less influence on resistance to powdery mildew on all cultivars. All genetic material found resistant to powdery mildew is currently used in peach and nectarine breeding programs in Romania.
Further studies focused on isolation and characterization of resistance genes for powdery mildew resistance will be done in the future based on data collected during several years.
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Scheduling of ornamental plant production
76-86.Views:128The production of ornamental plants represents an important branch of our horticulture. The growing area is relatively small (round 2800 ha), the production value, however, a rather large. Notwithstanding, its financial balance is inactive because the demand surpasses the supply copiously.
The most popular plants are as follows: carnation, gerbera, rose, bulbous flowers, chrysanthemum, other cutflowers, cutgreens and Gypsophyla — their total production value makes up to Ft 8-9 billion. The area of ornamental nurseries — about 800-900 ha — is to be found mostly on the western part of our country.
Our accession to the EU will have undoubtedly an impact on our ornamental plant production. We must take into account, that in greenhouse production the specialization extends all over the world, field production over a limited region. Our chances will not deteriorate by joining the EU. The buyer — chiefly because of ecological purposes — will prefer the domestic product to the foreign one. By the way, our products will be competitive, as far as quality or price is concerned, with those of western Europe. Last but not least, we may hope a greater saleability of home-bred, special varieties and cultivars, the so-called "hungaricums", both in the domestic as well as export markets. It is anticipated that we will have a good turnover with potted ornamental plants and flower seedlings. Our nursery products will become marketable too.
To exploit the opportunity, of course, the necessary conditions are to be created. First of all, we need development in research, with special regard to breeding, at the same time in education, in extension service, in the training of experts, on a high level. Some tasks can be solved, no doubt by improvement of the organisation within the branch. At the same time the state subsidy is indispensable in order to promote both the technical and the research activities. State subsidy is also necessary to build up more advantageous conditions of sales like in some foreign countries (e.g. the Netherlands).
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Epidemiological survey of Xanthomonas arboricola pv. juglandis and Gnomonia leptostyla on natural population of walnut (Juglans regia) in eastern Transylvania
7-9.Views:2077In Romania, walnut Juglans regia L. is an important fruit crop, although most of the fruit production comes from non-grafted walnut trees, which are natural hybrids. Breeding programs have been launched during last 30 years to develop new cultivars with uniform fruit quality. In addition, foreign cultivars have been introduced and tested to establish a valuable walnut genepool. To improve the present assortment of generative rootstocks in walnut and to examine the infestation level, a long term survey was carried out in Eastern Transylvania. The main physical characteristics of fruits and its variation to the infestation level were considered. The cumulative distribution of Xanthomonas arboricola pv. juglandis and Gnomonia leptostyla were relatively low and the maximum value was around 15%. The highest infestation of husk with X. arboricola pv. juglandis was observed for roundish forms and differences were statistically significant comparing with other phenotypes. Infestation with G. leptostyla was similar for roundish, elliptic and thwarting egg-shaped phenotypes, while the husk infestation for egg-shaped phenotypes was not observed. The walnut population studied in our experiment can be considered as a genetically valuable population. More than 20% of them have upper class quality fruits with at least or more than 50% of nutmeat. Do to the large scale climate variation in Eastern Transylvania and the high humidity favourable for pathogen infestations, these population can be considered resistant and well adapted to abiotic and biotic factors.
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Comparative evaluation of different valerian (Valeriana officinalis L.) lines
63-66.Views:157Research project to establish a Hungarian valerian cultivar with acceptable production, biological and chemical properties of five promising Valeriana officinalis L. lines were studied. The populations were not homogeneous especially with regard to leaf shape and colour. Line 'IV/1' showed the strongest vigour, was characterised by the highest root-yield (381.79 g/plant), volatile-oil (0.60 m1/100 g) and valtrat content (1.15%). However, the highest valeric-acid content (0.20%) was measured in another line, 'I/5'. According to our results, the above-mentioned lines (1/5', 'IV/1') could be the basic material of breeding work in the future.
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The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time
27-29.Views:243Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.
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The importance of clonal selection of grapevine and the role of selected clones in production of healthy propagating stocks
15-24.Views:290Genetical alterations and phytosanitary status promote the variability and modify the appearance of vine. Old vine varieties in old vineyards are highly variable and well adapted to selection. Clonal selektion is based on a visual performance: valuable individuals (clones) are picked out according to visible symptoms or characters. The genetical stability of clones is proved by testing the vegetatively propagated progenies on the basis of morphological and molekular (SSR, AFLP, SMPL, RAPD) markes. Authors take great care of the visual phytosanitary selection as part of the clonal selection being the oreliminary step to develop pathogen-free propagation stocks. In Serbia (Vojvodina) the selection breeding has been carried on for several decades resulted in comparative clone trials with home and imported clones of Welsch Riesling, Chardonnay, Pinot gris, White Riesling. Among the clones of home selection SK.54 Welsch Riesling clone is the most valuable. Its clearing from pathogene is being carried on in an interregional IPA programme (HUSRB/0901/214/123) in Kecskemét. In Kecskemét, the centre of the Hungarian Danube vine region 5 vine clones have been registered (Cegléd szépe K.73, Irsai Olivér K.11, Kövidinka K.8, Hárslevelû K.9, Pannónia kincse K.56). Besides them 18 virus-tested clones have also been qualified.Works aiming at their complete exemption are going on in order to obtain clones free of propagation wood-borne diseases.
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Self-incompatibility in plums (Prunus salicina Lindl., Prunus cerasifera Ehrh. and Prunus domestica L.). A minireview
137-140.Views:423Japanese plums (P salicina) and cherry plums (P cerasifera) are diploid species, while European plum (P. domestica) cultivars are hexaploids. Most diploid species are self-incompatible while fertility relations of the hexaploid European plums are variable between self-incompatibility and self-compatibility. About twenty S-alleles and six inter-incompatibility groups and one S-haplotype responsible for the self-fruitful phenotype were described in Japanese plum cultivars, but studies on cherry plums and even on the European plum cultivars are severely restricted. This review is focused on the available information obtained from myrobalans and European plums; and discusses recent hypotheses regarding the putative origin of the hexaploid plums, and thereby indicates the possibility of allele flow between different plum species.
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Antioxidant capacity, total phenolics and mineral element contents in fruits of Hungarian sour cherry cultivars
59-64.Views:355Several epidemiological studies revealed that the consumption of antioxidant compounds and the risk of atherosclerosis, increased blood pressure or cancer are inversely proportional. Fruits of sour cherry contain a wide range of antioxidant compounds including melatonin, perillyl alcohol, ellagic acid, several flavonoids, polyphenolics, and anthocyanins. This study was carried out to survey the antioxidant power and mineral element content of seven commercial sour cherry cultivars and three cultivar candidates and to assess the influence of some external conditions on fruits' functional properties. Our analysis revealed nearly 5- and 2-fold differences between the lowest and highest antioxidant capacities and total phenolics content, respectively. Some cultivars (`Kántorjánosi' and ‘Újfehértói fürtös') and cultivar candidates (D, 'Petri' and 'Éva') showed outstanding antioxidant capacity and total phenolic content; in addition, mineral element content in fruits of the ‘Újfehértói fürtös' cultivar was also favourable. Redox parameters of fruits were influenced by the cultivation plot or fruit positions within the canopy in about half of the cultivars tested. Genetic background of cultivars forms the decisive factor in determining fruits' antioxidant capacity, although external factors may have also sizeable modifying effects. Enhanced functional properties of the fruit may also be further increased through breeding programs since considerable variation exists within the tested germplasm.
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Fire blight (Erwinia amylovora) susceptibility of old Hungarian apple cultivars
35-38.Views:139The aim of the Hungarian apple breeding program started in the Department of Fruit Science was to find resistant apple cultivars against major diseases (scab, powdery mildew, fire blight). The outbreak of fire blight (Erwinia amylovora) in 1996 motivated us to search new resistant sources principally from old traditional apple cultivars. First of all, cultivars have been gathered since 1997 from Carpathia (Visk) and evaluated between 2001 and 2003. In this recent study evaluation of resistance of old Hungarian genotypes to fire blight collected from the English National Fruit Collection (Brogdale, Faversham) is presented.
13 old Hungarian apple cultivars in 2002, and 38 genotypes in 2005 have been evaluated. We used `Idared' and 'Jonathan M41' as susceptible controls and 'Liberty' and 'Remo' as resistant ones. Shoots of two-year-old potted plants were inoculated with a mixture of virulent E. amylovora isolates (Ea2, Ea60, Ea67) at a concentration of 5 x 108 cells/ml. Resistance of apple cultivars was evaluated weekly, four times after inoculation by disease severity of symptoms. Numbers of bacterial colonies in 1 cm length shoot were determined in the fourth week after infection.
8 cultivars in 2002 and 9 cultivars in 2005 displayed notable resistance to fire blight based on one-year data. Based on the coincident data of both years, out of the cultivars collected also from Carpathia ‘Pónyik', and `Sikulai' were found to be resistant and gene sources additional old Hungarian valuable apple genotypes could be selected: `Szabadkai szercsika' and `Tordai piros !davit'. The cultivar `Szemes alma' originated from Visk has proved to be recurrently resistant.
The number of bacterial cells in shoots of the investigated cultivars correlated with the severity of symptoms. With this method, which was introduced by us earlier, we can screen cultivars displaying weak visible symptoms, which cannot be proposed as a source of resistance because of their latent infection.
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Cultivars, marketing and integrated Production of apples in the Czech Republic
87-92.Views:108Development in apple production in the Czech Republic has been adversely effected during a few recent years by escape of capital investment from the agrifood industry (due to privatisation, restitution and transformation). On the other way round the competition on the domestic market is much higher than it was before because of unlimited import of subtropical and tropical fruit and some import of traditional fruit species as well. Therefore fruit growers are very much concerned in any possible means (which are not economically feasible) to be more competitive. One of the most common approach is the use of new cultivars bred in the country which are resistant or tolerant to diseases and offer better quality both for fresh market and processing. Another way for increasing competition ability for fruit growers is the join and mastering of integrated production which also considerably improve the quality of fruit as far as aspects of health are meant.
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Healthcare values and potential uses of the new Hungarian apple varieties on the basis on fruit analysis
25-28.Views:179Biological active compounds and valuable characteristics of some apple varieties and candidates were measured in our trials. Fruits of ’Rosmerta’, ’Hesztia’, ’Cordelia’ and ’Artemisz’ are recommended to enrich the Hungarian assortments for fresh consumption and choice of new tastes. Based on examined parameters it can be assumed that novel Hungarian resistant varieties are suitable for juice and fruit concentrate production, and due to high pectin content of their remaining pomace these varieties can be raw material of pectin production as well as they are also suitable for jam production mixed with other fruit species. Furthermore, functional food industrial product having high quality can be produced by using novel resistant varieties because of their high pectin and polyphenol content. Beside of their high market value their suitability for growing among orchard conditions is confi rmed by lower costs of production because of less plant protection treatments.