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Role of hydrogen peroxide and Pharmaplant-turbo against cucumber powdery mildew fungus under organic and inorganic production
39-44.Views:410Cucumber leaves have been sprayed with a solution of hydrogen peroxide (H202) or Pharmaplant-turbo combined with organic or inorganic fertilizers under plasic house. Under the influence of H202, leaves exhibited resistant against Podoshaera fusca fungus, the causal agent of cucumber powdery mildew. H202 (15 mM) was able to decrease the disease severity from 90.4% to 12% in two experiments conducted in two seasons. Pharmaplant-turbo (Turbo) is new chemical compound and used as an antifungal compound. Turbo in 1 ml/L was able to decrease the disease severity from 90.4% to 11.5% in the both experiments as well. Both of H202 and Turbo were combined with organic treatment (compost + compost tea + seaweed extracts) which showed significant effect against cucumber powdery mildew fungus and strongly suppressed it as compared to control leaves. Organic treatment produced higher vegetative growth characters and greater early and total yields as compared to inorganic treatment, also organic fruits produced the lower nitrate content and the higher ascorbic acid content as compared to inorganic fruits. Our study have indicated that, H202 and Turbo combined with organic fertilizers play a role in the resistance of cucumber against powdery mildew by decreasing the disease severity. We suggest to give more attention to the direct application of H202 in low concentration and Turbo against powdery mildew diseases and other plant diseases.
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Investigation of flowering dynamics of the basil (Ocimum basilicum L.) and its consequences in production
74-80.Views:158The flowering dynamics of Ocimum basilicum L., (a common population maintained at the UHFI) was studied with the aim to create an exact and practically applicable method for definition of phenological phases.
According to our observation the development of individual flowers of the basil can be characterized by 8 distinguishable phases, which must be considered for description of the actual phenological stage of a spike.
An accurate model was created for the unambiguous description of flowering process of different flowers within the spike and for the individual plant as a whole. The new flowering index formula is calculated from the number of flowers weighed by their phenological phases.
The time dependence of flowering is presented by functions fitted to values of the flowering index. The results reflected different patterns of the main inflorescence, the inflorescences formed on the side shoots in the first or in the second half of the flowering period. However, for description of the flowering process of the whole plant, a sigmoid function proved to be the appropriate model.
The accumulation process of the essential oil could be characterized by the flowering index values. They showed close correlation (r=0.964) at high probability.
The new method assures an exact definition of phenological phases in basil. Its application seems to be optimal during breeding procedures, seed production and even in production of high quality drugs, according to the requirements of the Good Agricultural Practice (Guidelines for GAP, 1998).
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Maceration affects mineral composition and pH of wines
25-29.Views:240Mineral composition of wines is affected by many ecological and technological factors. These variables are mostly discussed focusing on certification of origin and human health. This paper gives data on possible changes in mineral composition and pH of wines due to 4 hours skin maceration (1st trial) and fermentation sur marc (2nd trial). Experimental site is situated on acidic sandy soil in middle east Hungary. The variety collection was established in 2003 with own rooted planting material in 3x1 m spacing, trained for single curtain training
system. Mineral composition of wines was determined with ThermoFischer Scientific iCAP 6300 ICP-OES, pH was measured with pH10pen (VWR International) in field, and with SevenEasyTM pH meter (Mettler Toledo) in laboratory. 1st trial with 9 PIWI white wine grape varieties pointed to demonstrate effects of skin maceration, which is abundantly used to elevate aroma content. 2nd trial with the use of ‘Medina’ red PIWI grape variety aimed to demonstrate effects of double pasta skin maceration in rose and red wine technology. Data of 1st trial show, that K, Cu, Mn and P respective increase with skin maceration, despite Fe show considerable decrease. Data of 2nd trial show, that K, Mg, Mn, P, Sr and B increase with longer skin contact and higher fermentation temperature, despite Fe and Ba decrease with this technology. In regard to pH, data show, that skin maceration and fermentation sur marc increase K content with about 30-70% respectively, withstanding that pH also increase with a considerable 0,4-0,5 value. K content can be higher in the end product, despite to earlier higher level of tartrate formation. An attention should be driven to lower level of tartaric acid and consequently higher pH characteristic for wines produced with the application of skin maceration or fermentation sur marc. -
The effect of Regalis® (prohexadione calcium) on the reduction of fire blight (Erwinia amylovora) severity in apple trees
61-66.Views:162Due to the lack of effective and non-phytotoxic materials for control of the blossom and shoot blight phase of fire blight in pome fruit trees, two novel control strategies have emerged: shoot growth retardation by bioregulators and applying resistance inducer compounds. Prohexadione calcium (ProCa) is the active ingredient of the bioregulator Regalis® registered in several European countries. The reduction of shoot growth elongation is the most obvious effect of ProCa. Furthermore, it causes significant changes in the spectrum of flavonoids and their phenolic precursors, leading to the considerable reduction of susceptibility to fire blight.
In Poland, potted one-year-old apple trees of cvs. Gala Must grafted on M.26 and Sampion on M.9 (in 2001) as well as Gala Must on P.60 (in 2002) were treated with Regalis® at a range of concentration of 250, 150 or 150 + 100 ppm, respectively. The inoculation of shoots was made with the strain No.691 of E. amylovora (107 cfu/ml), on the 7th and 21st'day after treatments with Regalis. In Hungary, during the years of 2002 and 2003 one-year-old container grown apple trees of the cvs. Idared/M.9 and Freedom/M.9 were treated with the prohexadione-Ca, the active ingredient of Regalis® 100, 150 or 200 ppm, two weeks before inoculation with the Ea 1 strain of E. amylovora (107 cfu/m1).
In Poland, the suppression of fire blight in shoots reached up to 80%, depending on concentration and application time of Regalis®. In Hungary, the effect of prohexadione-Ca treatments, determined by the length of necrotic lesion developed, proved to be better than that of streptomycin used for comparison.
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Effect of 1-MCP and chemicals on the vase life of Rosa hybrida L. cv. 'Bordeaux'
13-16.Views:146The effect of 1-MCP on rose cultivar 'Bordeaux ' was studied in combination with different preservative solutions. The flowers were treated with 1-MCP for 6 hours at 17 °C and for 18 hours at 4 °C. As for the diameter of flowers the best resu lts was achieved, when applying 20 gL- 1 sucrose + Clorox (2 ml L- 1), as well as preservative "Spring" ( I 0 gL-1) + 1-MCP ( 18 hours). Similar results were measured in the case of Spring + Clorox + 20 gL-1 sucrose with 1-MCP (6 and 18 hours). The longer MCP treatm ent gave better results as compared to the shorter ones, because the flowers were much more open.The condition of flowers was the best in the solution of Clorox + 20 gL-1 sucrose and Spring solution combined with the longer MCP treatment. and Clorox + 20 gL- 1, on the IQ1h day. The longest vase life ( 10,3 day) was observed in the case of Spring application combined with 18 hours of MCP treatment. ln this treatment the vase life was prolonged by 3,3 days. As regards the calculated ornamental values, the following sequence could be established :"Spring" + l-MCP ( 18hrs) 134 points, Clorox + 20 gL-1 sucrose + 1-MCP (l8h) with 129 point, and the third was the Clorox + 20 gL-1 sucrose with 1 19 point.
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Application of pollen mixing device in front of the hive entrance
151-152.Views:122The model of a pollen mixing device was tested in sunflower culture in order to detect the effectiveness in helping the out-going honey bees to carry more and different varieties of pollen grains on the body and body hairs. This model is build from line hairs of brushes facing each other that can keep and release pollen grains. The brushes are functioning as pollen receiver-transmitter units. The effectiveness was tested by examining the number and the species of the pollen grains adhered to the bees passing through the entrance. The amount of pollen and the number of pollen species on the hive with the device was significantly higher than on the control hive.
The data were analysed by variance analysis and significant differences were found according to the number and species of pollen grains between the incoming and outgoing bees.
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Aminoglycoside antibiotics affect the in vitro morphogenic response of chrysanthemum and tobacco
93-104.Views:109Broadly the success of genetic transformation of plants requires non-chimeric selection of transformed tissues and its subsequent regeneration. With rare exceptions, most plant transformation protocols still heavily utilize antibiotics for the selection of transgenic cells containing an antibiotic-degrading selectable marker gene. The morphogenic capacity of in vitro chrysanthemum and tobacco stem and leaf explants change with the addition of aminoglycoside antibiotics (AAs). Of 6 antibiotics tested, phytotoxicity occurred at 10-25 and 50-100 pgml-I in chrysanthemum and tobacco explants, respectively, depending on the size of the explant and the timing of application. The presence of light or darkness also had a significant effect. The use of transverse thin cell layers (tTCLs) in conjunction with high initial AA selection levels supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow cytometric analyses demonstrate that regeneration can be predicted in both species, depending on the ploidy level of the callus. Endoreduplication was not observed in chrysanthemum, even at high AA levels, but occurred (8C or more) in tobacco callus, even at low AA concentrations (5-10 pgml-1). The higher the AA level, the greater the DNA degradation and the lower the 2C and 4C values.
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Effects of biochar and inorganic fertiliser on the growth and yield of beetroot (Beta vulgaris L.) in Kenya
37-45.Views:174Beetroot (Beta vulgaris L.) is a root vegetable packed with many nutritional benefits such as minerals and vitamins. Despite its importance in Kenya, farmers get about 30-35 t/ha which is significantly lower than the potential yield (68 t/ha). This is mostly attributed to low soil fertility. This study aimed to determine the response of the beetroot growth and yield on biochar and NPK. A 3×4 factorial experiment was carried out at Egerton University farm over two seasons to test the effects of biochar and NPK (17-17-17), under supplemental irrigation. Biochar (0, 5, 10 t/ha) was combined with NPK (0, 200, 300, 400 kg/ha). The combination of Biochar and NPK increased significantly (p ≤ 0.05) beetroot growth and yield in two seasons. Treatment B10N400 showed the tallest plants (79.2 cm) at 90 days in season two, while the control resulted in the shortest (27.6 cm). Treatment B10N200 showed the biggest (213.2 cm2) leaves at 90 days. The treatment B5N300 recorded the highest marketable yield (84 t/ha) in season two and the lowest was B0N0 with 2.6 t/ha. Sole application of NPK rates (200, 300, 400 kg/ha) increased significantly the growth and yield of beetroot compared to the control in both seasons. In season one, N300 (300 kg/ha) had 61.9 t/ha of the total yield, the control had the lowest. In season two, 300 kg/ha had 83 t/ha of total yield. Biochar increased beetroot growth and yield in season 2. Treatment B5 recorded the highest marketable yield of 61.2 t/ha, while the control showed the lowest of 53 t/ha.
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Role of organosilicone surfactant in apple scab control under scab conducive weather conditions
23-25.Views:170Fungicides of integrated fruit production (dithianon, captan, and diclofluanid) and an organosilicone surfactant were compared in spray schedules from green tip until summer in order to control apple scab caused by Venturia inaequalis and to evaluate their phytotoxicity on fruit. Sixteen sprays of 1.8 kg ha-1captan, 0.41 ha-1 dithianon, and 1.8 kg ha-1 diclolfluanid significantly (P=0.05) reduced the incidence of leaf or fruit scab compared to unsprayed products. All fungicides applied with organosilicone at 0.1% resulted in lower incidence of scab on young and older leaves as well as on harvested fruit, but these were not statistically always better than fungicides applied alone. In case of diclofluanid, the fungicide applied with organosilicone at 0.1% resulted in significantly lower (P = 0.001) incidence of scab on young and older leaves. Diclofluanid applied with organosilicone at 0.1% gave the best scab control on leaf and fruit. Treatments applied with fungicides alone had no significant effect on plant phytotoxicity compared to untreated control. All fungicides applied with organosilicone at 0.1% increased (P = 0.05) fruit damage compared to untreated control or fungicide applied alone, though these were not always significantly different. In case of percentage of fruit russet, treatment of dithianon 0.4 1/ha + 0.1% organosilicone significantly increased fruit russet, while fruit russet index significantly increased in the treatment of diclofluanid 1.8 kg/ha + 0.1% organosilicone compared to untreated control. In sum, application of surfactants can help to increase efficacy of scab fungicides; and consequently, to reduce the risk of fungus infection under high scab disease pressure. This fact may also be helpful in fungicide resistance management and reduced-spray programs with accurate scab warning systems.
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Growing characteristics of apple cultivars in environmentally friendly growing systems
7-10.Views:225Nowadays the condition of the good saleability of the fruit is the application of controlled environmentally friendly technologies. Success of the growing is basically influenced by the production value of the cultivars, and their flexibility to the different technologies. In our examinations the effect of the integrated and organic farming system has been evaluated on the growing characteristics of the apple cultivars. According to our results the trunk thickness of the trees both in the initial and both in the later bearing period is higher in the integratedproduction compared to the organic farming system. This differences most likely caused by the variance of the crop load.
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The effects of foliar nutrition containing various macro and microelements on the growth and development of young grafted walnut (Juglans regia L.) plants
33-37.Views:153The Stopgril liquido (S), a foliar fertilizer containing nitrogen, calcium and magnesium was used to improve the spring and the summer growth of grafted walnut plants in the second year of cultivation in the nursery. To accomplish early termination of vegetative growth and good lignification, the composed mineral fertilizer Hascon M 10 AD (H) containing phosphorous, potassium and microelements B, Mn and Mo was applied on the same plants. Four treatments (S, H, SH, C) were applied three times in a growing season. Their effects on the height and the basal diameter of the plants in the nursery depended on the starting height of the plants and the time of application. During the first growth period in the orchard, the number of developed buds on the plant, the circumference of the plants and their height were influenced by the treatment in the previous year in the nursery. According to the results obtained through the investigation, three applications of Stopgril + Hascon in the nursery per year can be recommended in order to obtain high enough and well lignified two-year-old grafted walnut plants.
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Floral bud development, blooming time and fertility relations of some Romanian apricot varieties in Hungary
41-43.Views:232Due to the geographical situation of Hungary the introduction of late ripening apricot varieties holds great interest. In apricot production floral bud development during winter, blooming time, and the fertilisation properties are important factors. These characters were studied in six late ripening Romanian apricot varieties (Callatis, Comandor, Litoral, Selena, Sirena, Sulmona) in Szigetcsép representing the northern site of the lowland growing area. During the mild winter of 1997/98 the dynamics of floral bud development in the Romanian varieties was a few days slower during the whole examination period compared to Gönci magyar kajszi. Their meiotic divisions occurred between 1 and 5 February. Next winter the meiotic division started later at 28 February, due to the cold weather. In these conditions the dynamics of bud development was similar in all the varieties. Averaged over seven years blooming of the Romanian varieties started 1-3 days later than in Gönci magyar kajszi. All the Romanian varieties showed self-fertility to some extent, however, application of other pollen donor sources is necessary for the safety of production, with the exception of Callatis.
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Historical background and constraints of a grapevine germplasm foundation in Hajdú-Bihar county, Eastern Hungary
11-17.Views:245The historical background of Debrecen linked to viticulture and wine-making stands mainly on the lack of drinkable water, the necessity of drinkable liquid during wartime and epidemics. The special character of the city evolved together with the changing lives of citizens and the increasing trade importance of the city. Period of Turkish occupation gave impetus to the formation of the 11 vine gardens of the settlement. After the devastation of rootmite and peronospora ‘Kadarica’ and ‘Nagy burgundy’ (‘Blaufrankish’), in smaller proportion - on lower sites – ‘Cabernet’ were planted. As white varieties ‘Ezerjó’, ‘Olasz Rizling’, ‘Kövidinka’, ‘white Mustos’, in smaller proportion ‘Szlankamenka’, ‘Erdei’, ‘Szilvaner’, ‘Mézesfehér’, ‘Bakar’, ‘Veltelini’ (red), ‘Fehér burgundi’ (? white burdunder), ‘Rajnai rizling’, ‘Red Tramini’, ‘Furmint’, ‘Muscat Lunel’, ‘Járdovány’ and ‘Juh-fark’ were planted. After the Trianon treaty in 1920, 2/3rd of Hungary was cut away. Érmellék wine region was also cut in two, thus Debrecen broke away from its wine region. Legal regulations after the World War II. (1959) referred back to variety application advised in 1924 for “place suitable for good wine production, not included in any wine region”, like Debrecen listing ’Ezerjó’, ’Mézesfehér’, ’Olaszrizling’, ’Bánáti rizling’, ’Furmint’, ’Hárslevelű’, ’Kövidinka’, Kecskemét virága’, ’Piros szlankamenka’,’Pozsonyi fehér’; ’Kadarka’, ’Oportó’ and ’Kékfrankos’ (Blaufrankish). The political changes of 1990 and Hungary’s admission to the Eurepoean Union almost annihilated the wine production of Debrecen. However little gardens conserved historic varieties which could date back even to many centuries. Through a local magazine a collecting work was announced pointing to gather ancient local (Vitis vinifera conv. pontica) varieties forming a genebank, established on the experimental station of the University of Debrecen. In 2014, about 112 items were collected (accessions). As a 2nd round of the work, with a more detailed and precise work, further 81 items were put into the reservatum. The latter represent single stuck collection, whereas the first ones are to be studied az mixed items. Most notable accession names (ACENAME) of the work are: ‘Fehér gohér’, ‘Veres gohér’, ‘Fekete gohér’, ‘Kék gohér’, ‘Erdei’, ‘Ezerjó’, ‘Kűbeli’, ‘Rizling’, ‘Mézes fehér’, ‘Dinka’, ‘Madling’, ‘Bakator’ and ‘Kadarka’. Simulteneously with the strenghening and morphological description of conserved stucks genetic identification of the items is being elaborated. Database comprising FAO/IPGRI multi-crop passport descriptors and OIV Primary descriptor priority list are to be published on-line in between the development of the platform.
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Application of DNA markers for detection of scab resistant apple cultivars and selections
59-63.Views:103A DNA marker-based study was undertaken to identify the occurrence of major scab resistance genes in some apple cultivars and selections of importance for apple breeding. Unfortunately none of the RAPD-based markers previously reported to detect the Va, Vb, Vr and Vx genes produced unambiguous results. By contrast, the CAPS marker MI8 produced the expected three bands in all cultivars and selections already known or suspected to have the Vf gene, as well as in the Russian cultivar 'Antonovka Polotora Funtovaja' suspected to have Va resistance which however may be allelic to Vf. Vf-carrying selections and newly named cultivars 'Frida' and 'Fredrik' are grown successfully in Sweden without fungicides, suggesting that the Vr resistance breaking scab races 6 and 7 have not yet become a problem. The SCAR marker B12 detected the Vm gene in 'Prairifire', 'Rouville', clones 'OR45T132' and 'OR48T70', and selection '16-36-193'. The SSR locus 0102b10 detected one band at 118 by in 'Reka'. This is presumed to be identical to the Vr gene marker previously reported.
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Technologies developed to avoid frost damages caused by late frost during bloom in the fruit growing regions of Siófok and Debrecen
99-105.Views:341The aim of the study was to fi nd out which of the methods used to avoid damages of late frosts would be the most effective for the fruit growing practice. Three technologies have been tested in the regions of Siófok and Debrecen-Pallag. The antifrost irrigation proved to be the most advantageous. For that purpose microjet sprayers are used, which are thrifty and do not need for that purpose large containers. With the aim to secure an even distribution of water, the sprayers are distributed on three levels: above and inside of the crown as well as on the level of trunks. On a large scale, a single microjet above the crown level would be suffi cient. By means of a detailed analysis served to set the optimum intervals between spraying phases: with each 15, 10, 5 and 3 minutes during half a minute. The synchronous presence of water and ice below the freezing point, the released freezing heat plus the water used much above the freezing point (9–10 °C) altogether maintains the temperature above around 0°C near the fl owers or growing fruitlets, meanwhile, the surrounding air cools down to –8 °C. The effectiveness of the generally used anti-frost would be increased substantially by the former application of cooling irrigation, which delays the blooming date. The Frostbuster represents a new technology developed for the same purpose of frost defence. It uses butane burning and produces high (80-90°C) temperature with a strong blast of air. On the protected area sensors have been distributed for measuring temperature and relative humidity. The means of the measurements proved a rise of temperature, which was suffi cient to save the fruit trees until the difference is less than –3–3.5 °C. Our analyses stated that paraffi n candles avert the frost until –4 °C. Its success depends largely on the intensity of air movements. A small wind would be enough to frustrate the effect. The results show clearly the utility of Frostbuster and paraffi n candle combined is approved.
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Insecticidal effect of oregano (Origanum vulgare L. ssp. hirtum Ietswaart) on bean weevil (Acanthoscelides obtectus Say)
84-88.Views:1650The effectiveness of drug plant and of essential oil of oregano (Origanum vulgare L. ssp. hirtum Ietswaart) against the adults and egg hatching of bean weevil (Acanthoscelides obtectus Say) was studied in laboratory trials in order to screen for a new potential insecticide in the control of this granary pest of beans. The adult mortality as well as oviposition and hatching capabilities were evaluated after 6 days of treatment of beans cv. `Berggold' (55 g) in Petri dishes by powdered drug (0.33 g, 0.66 g, 1.0 g and 2.0 g) and by equivalent amounts of essential oil (5 ill, 10 15 ill and 30 ill). Oregano essential oil showed both fumigant and contact toxicities to bean weevil, the later being more potent. When considering fumigant toxicity, insecticidal effect (mortality rate 82.5%) was observed 6 days after application of high concentrations of oregano essential oil (150 Al per 55 g of beans). When considering contact toxicity, both oregano drug plant and essential oil at all tested concentrations significantly increased the bean weevils' mortality rates with comparison to the controls. Also, egg laying and hatching were inhibited after treatment of bean weevil with powdered drug plant or with essential oil at all tested concentrations. Insecticidal effect of drug plants issuing from two different stocks depended on their essential oil contents. Essential oils (5 pi, 15 pi and 30 IA per 55 g of beans) induced 100% mortality of bean weevil population when applied directly to the surface of beans (55 g) in Petri dishes. The content of essential oil in oregano and oregano-based preparations for insecticidal use is proposed as a parameter for quality control purposes.
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Cracking susceptibility of sour cherry (Prunus cerasus L.) in Hungary and relation to calcium application
109-118.Views:278The rain induced fruit cracking is a big, serious problem especially for sour cherry growers but in some year sour cherry growers had also problem with fruit cracking caused by too much rainfall in the harvesting season. The cracked sour cherry fruits can be easily infected by different diseases like Monillinia sp. Cracked and infected fruits can not be transported for long distance and using for preservation because they lost their market value by the pour fruit quality. There are two possibilities to protect fruits against the rain induced fruit cracking. The most effective protection technique is the plastic rain cover over the tree rows. The installation of these equipments is too expensive for the growers. That is the reason why researchers tried to find other less expensive and sufficiently effective ways like sprayings different mineral salts, hormone and other type chemicals against the rain induced fruit cracking. Several calcium formulas calcium chloride (CaC12), calcium hydroxide (Ca (OH),) and calcium nitrate (Ca(NO3)2) can be sprayed in appropriate solution concentration.
The aim of our trial is to determinate the fruit cracking susceptibility of wide grown Hungarian sour cherry varieties and find the most effective calcium formula and its concentration for spraying in orchards to prevent the fruit cracking. In the first trial year (2006) cracking index of tested sour cherry varieties were determined under field and laboratory conditions. Under field conditions were not found differences between cracking tendency of tested cultivars. After results of immersing fruits in distillated water for 24 hours tested sour cherry varieties were divided to three groups by the susceptibility to rain induced fruit cracking: very susceptible (`Maliga emléke', 'Piramis', 'Érdi jubileum', 'Erdi nagygyümölcsű’ and 'Meteor korai'); susceptible ‘Érdi bőtermő, Tandy 279' and Cigány 59.; moderately susceptible/tolerant ("T" and "R" clones). In the second trial year (2007) calcium chloride (CaCl2) and calcium nitrate (Ca(NO3)2) were sprayed in 0,35m/m%; 0,5m/m% and 1,0m/m% solution concentration. One more commercial product "Damisol-Kalcium" was also applied in the advised 1,0m/m% concentration. Like in the trial year before (2006) under field conditions we did not kept differences between the cracking susceptibility of varieties and calcium treatments. As the result of laboratory testing (immersing calcium treated fruits in distillated water) we kept that calcium chloride (CaCl2) seems the most effective against the fruit cracking in 0,5m/in% solution concentration. The other calcium formulas also decreased the cracking ratio but in less scale.
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Selenium enriched vegetables as biofortification alternative for alleviating micronutrient malnutrition
75-81.Views:472There is a very difficult equation for malnutrition and over-consumption. That means malnutrition even of vitamins and/or minerals (Ca, Cu, Fe, I, Mg, Se and Zn, vitamin A) affects more than two billion people worldwide, largely due to low concentrations or poor bioavailability of the nutrients in the diet. In some developed countries in contrast, over-consumption, particularly of over-refined cerealbased foods, has contributed to the development of an epidemic of metabolic diseases. So, producing nutritious and safe foods sufficiently and sustainably is important target at the same time challenge of modern agriculture. In the past, great efforts have focused only on increasing crop yields, but enhancing the concentrations of mineral micronutrients has become an urgent task. The main daily food source is the staple crops specially in developing countries of the world, i.e., wheat, rice, cassava, beans, sweet potato or maize. These kind of plants are often deficient in some of mineral elements. Thus, the increasing of bioavailable concentration of micronutrients in edible crop tissues (via biofortification) has become a promising strategy in modern agriculture, providing more nutritious foods, to more people, with the use of fewer lands. Biofortification of these trace elements can be achieved application with agronomic process such as soil or foliar fertilization or crop breeding even conventional technic and/or genetic engineering. This review highlight progress to date and identify challenges faced in delivering biofortified vegetable crops as well as the agronomic approaches and tools to improve crop yield and micronutrient content of food crops.
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Experimental approach in apple tree nutrition
9-13.Views:123Authors present synthesis of experimental work, performed in the last decades, for better understanding nutritional behaviour of apple trees and related problems in fruit quality. There were evidences supporting possible deteriorating role of potassium in feeble physiological status of apples, if applied in excess. More intensive studies proved that higher potassium uptake into leaves and fruits might be also the result of increased sink power of individual fruits. Nevertheless early senescence of apples during storage and also sensibility to bitter pit were successfully related to the increased sink power of fruits, casual relations in excessive NPK fertilization, although increase in sink power need further investigations. Impaired weather conditions during early development of fruits, hostile orchard practices in pruning, thinning, irrigation and also unskilled application of growth regulators may also contribute in the enhancement of sink power and in weakened physiological status of apple fruits.
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New evaluation method to detect physiological stress in fruit trees by airborne hyperspectral image spectroscopy
37-40.Views:163Nowadays airborne remote sensing data are increasingly used in precision agriculture. The fast space-time dependent localization of stresses in orchards, which allows for a more efficient application of horticultural technologies, could lead to improved sustainable precise management. The disadvantage of the near field multi and hyper spectroscopy is the spot sample taking, which can apply independently only for experimental survey in plantations. The traditional satellite images is optionally suitable for precision investigation because of the low spectral and ground resolution on field condition. The presented airborne hyperspectral image spectroscopy reduces above mentioned disadvantages and at the same time provides newer analyzing possibility to the user. In this paper we demonstrate the conditions of data base collection and some informative examination possibility. The estimating of the board band vegetation indices calculated from reflectance is well known in practice of the biomass stress examinations. In this method the N-dimension spectral data cube enables to calculate numerous special narrow band indexes and to evaluate maps. This paper aims at investigating the applied hyperspectral analysis for fruit tree stress detection. In our study, hyperspectral data were collected by an AISADUAL hyperspectral image spectroscopy system, with high (0,5-1,5 m) ground resolution. The research focused on determining of leaves condition in different fruit plantations in the peach orchard near Siófok. Moreover the spectral reflectance analyses could provide more information about plant condition due to changes in the absorption of incident light in the visible and near infrared range of the spectrum.
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Phyl-Gold: a product to diminish russeting of 'Golden Delicious' apples
131-133.Views:128Producing high quality apples in mature trees of the cv. 'Golden Delicious' is rather difficult because of the russeting of it's fruits, especially in seasons of high relative humidity and/or wetness.
Earlier experiments proved the possibility to decrease russeting by treatments of GA4.7 during a period after petal fall.
Phyl-Gold, a product of Phylaxia Co. (ai. 10 g1-1 GA4.7) was applied to inhibit russet formation in fruit skin of 'Golden Delicious' apples. Four consecutive sprays were carried out (with 10 ppm a.i.) in weekly intervals, starting at petal fall and tank-mixed with the current pesticides of the plant protection program.
Due to the GA4.7 application the russet formation decreased at a rate of economical importance. As for the side-effects of treatments, fruit set was reduced slightly in trees treated, however, there was no consequent influence on return bloom.
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Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:183A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
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The effect of nitrogen supply on specific yield and fruit quality of apple (Malus domestica Borkh.)
7-21.Views:166The aim of the present study was to determine the effect of nitrogen supply on yield and fruit quality of apple cultivars and to explore the relationships between canopy density and the different fruit quality parameters.
The experiments were carried out at Kálmánháza, in Eastern Hungary in a private orchard in 2003-2004. The response of four apple cultivars ('Elstar', 'Gala Prince', 'Granny Smith', 'Idared') to different nitrogen dosages was studied. The assessed and calculated indices were: yield, fruit diameter, fruit height, shape index, fruit mass, firmness, dry matter content and colouration. The results indicated that nitrogen fertilization has a significant effect on the yield and fruit quality of apple cultivars. The calculated specific yield values were reduced by the application of nitrogen via the increase in the volume of the canopy. An opposit trend was observed for fruit diameter, fruit height and fruit weight, which increased with increasing nitrogen supply. The reduced shape index caused flattening of fruits.
However, the improvement of fruit quality via increasing nitrogen dosage is only virtual, since these dosages increase the fruit size, but firmness, dry matter content and colouration are diminished, which decreases the value of the fruits on the market.
The authors also studied the relationships between canopy density responsible for assimilation and light supply of the fruits and the different fruit quality parameters. The closest linear inversely proportional relationship was found in the case of colouration. There was a negative linear relationship between canopy density and firmness or dry matter content. The relationship between canopy density and fruit mass could be described by a quadratic polinomial function.
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Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.
65-71.Views:134The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.
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Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis
Views:187Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.