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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:198The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids
39-45.Views:177Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated.
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Variation in selenium tolerance among two onion cultivars in closed fortification system
75-77.Views:188Selenium (Se) is an essential trace element for humans and animals. To consumption of selenium could be good sources the vegetables. Many of them are able to convert the inorganic selenium forms to organic forms, which are more effective for health. To enrich onion with selenium is known however some unclear points are remained. A closed fortification system was conducted to compare the difference of selenium tolerance in two onion cultivars in greenhouse environment. This system was well controlled, eliminating a lot of disturbing factors. The comparative analysis of Makói bronz and Makói lila spring onoins showed that there is difference in selenium tolerance and accumulation not only between plant species but inside it between the different cultivars, too. The Makói bronz seemed to more sensitive to the selenate treatment than Makói lila.
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Hungarian fruits and vegetables of high anti-oxidant activity as functional foods (Review article)
13-21.Views:260Recently, projects aiming to enhance the consumption of fruits and vegetables are intensified. Experts agree in the principle of fresh vegetable foods being a valid panacea in averting risks of ailments as well as curing immune-insufficiency, inflammations, moreover, certain cancerous processes. It is generally accepted that among substances of biological activity the anti-oxidant compounds such as vitamins C, E and carotenoids, etc. have the major role in this process. Hungarian agriculture has outstanding chances in utilising its natural as well as cultivated plant resources and favourable climatic conditions. It would be, however, necessary to build up a databank of antioxidant substances found in fruits and vegetables and including the modifying effects of technology, growing site, variety, etc. The concept of promoting the trade of Hungarian food-specialities as "Hungaricum" needs, urgently, the aid of a databank of that kind. Some of those excellent products are for instance the sour cherry, pepper and onion. They enjoy high priority as "Hungaricum" in the EU and it should be enhanced by intense and consequent research work, which may prove their role as functional foods. The USA is the leading country in research on the anti-oxidant substances of sour cherries, and up to now more than 17 compounds have been found in Hungarian varieties among others. In pepper fruits used as vegetable and source of vitamin C, the analyses are still lacking because research of the past concentrated on the products of milled spice pepper. Onion and garlic are entirely unexplored in this respect. It should be noted that availability of these fresh products in the moderate climate is restricted to a relatively short season. For that reason, some processing and preservation methods are needed in order to use those fruits and vegetables as functional foods all around the year. The scientifically founded endeavour as a solution of the questions mentioned is stimulated by vigorous commercial interests as well as by the urgent needs of the consumers to improve their health.
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Effects of Foliar Nutrition on Onion Seed Storage under Controlled Atmosphere
101-110.Views:202Storage of fresh fruits and vegetables prolongs their usefulness. The principal goal of storage is to preserve the commodity in its most useable form for the consumer. The extension of storage life and the improvement of quality of fresh fruits and vegetables can be supplied by harvesting at proper maturity, control of post-harvest diseases, chemical treatments, refrigeration, controlled and modified atmospheres. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on onion seeds and storage under controlled atmosphere. The germination percentages were 85.50, 86.85, 83.75 and 82.15 for CA1, CA2, CA3 and CA4, respectively. Water content of onion seeds was significantly increased with decreasing storage temperature in all controlled atmospheres. Water content values were 4.48, 4.40, 4.55 and 4.54 for CA1, CA2, CA3 and CA4, respectively. There were significant decreases in water content with increasing exposure periods. The lowest water content was recordednfor the exposure periods of 120 and 240 days. Catalase and peroxidase activities were decreased with the decreasing of storage temperature, whereas malondialdehyde content was increased. The exposure for 120 days recorded the highest catalase activity. The highest value of peroxidase (11.72) was recorded for CA2. Peroxidase activity increased as exposure period increased until 120 days and then decreased. Cold storage temperature (5°C) showed the highest malondialdehyde content followed by room temperature. The CA2 showed the lowest malondialdehyde content compared with other controlled atmospheres. The exposure period of 120 days had the lowest malondialdehyde content.
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Strategy of the sour cherry verticum in the Northern Great Plain Region Hungary (Analytic study)
7-31.Views:213Sour cherry growing and consumption grows dynamically around the world. The present volume of 1 million tons will incerase within 10 years with 20-30, or even with 50%. In the world wide sour cherry production, Europe is a decisive factor, i.e. 2/3 of the volume is grown there. Prominent capacities are concentrated in East-Central Europe, mainly Poland, Germany and Hungary. In the future, new concurrent exporters are expected on the European market as Turkey, Iran, Serbia-Montenegro. Hungarian sour cherry production has rich traditions, so the growing techniques and the assortment of sour cherry varieties make Hungary a „Great Power" on this field. Fresh fruit and products developed from sour cherry represent values distinguished as „Hungaricum" on the markets. Sour cherry growing and the path of its products are one of the „pulling branches" of Hungarian fruit growing. Sour cherry occupies 15% of area for fruit growing and 40% within the stone fruits. Sour cherry was grown widely in Hungary, it was grown everywhere as for utilizing waste areas. This is the main reason that yields are low as a mean of 15 000 ha and the volume is low (50-60 000 tons) only. To that poor figure the heavy infections of Monilia contributed substantially in the last couple of years. The two most valid arguments of using the present varieties as the best solution are 1) the cross bred new varieties, and 2) the selections of local, traditional varieties, which substituted the earlier dominant 'Pándy meggy' variety, which had a good quality but yielded poorly. Sour cherry growing of Hungary shifted from the dry regions of the country toward the cooler and more humid regions, where the weather excesses secure a less risky production. The most decisive region is the Norther Great Plain Region comprising SzabolcsSzatmar-Bereg county, where more than the half of the Hungarian sour cherry volume is produced, and which is bound to increase its production in the future. The majority of sour cherry produced in Hungary is processed, moreover, an important fraction of the exported fresh fruit is also used by the industry. The main importer of Hungarian sour cherry is Germany. The industry manufactures mainly canned products, a smaller fraction will be processed to other products. The expected volumes of sour cherry grown in Hungary in the next 5 and 10-year-period was estimated from data based on the ratio of young plantations, predicted consequences of the global climatic changes, phytosanitary aspects, furthermore, on the development of the technological level. In the region, the volume grown within 5 years, 40 000 t/year will increase within 10 years to 55 000 t/y. The processing in Hungary is not sufficiently differenciated, which is attributed partly to the characters of the varieties, partly to the weaknesses of the processing industry. One of the reasons is the suitability of varieties mainly for canning products. Processed sour cherry products could not be sold at the same price levels achieved by concurrent sour cherry growing countries. The vertical structure of the path of products of sour cherry disposes of adequate processing capacity being ready to be developed or there is sufficient intention of making investments for the purpose of manufacturing special sour cherry products. Significant tasks of development are actual in the field of the ecological and biological conditions of production. Volume and yield security as well as the maturity time and diversification of processing possibilities are the main endeavours in widening the assortment of varieties to be grown in the near future. The main objective in growing techniques is the modernization of phytotechnical procedures, and new solutions of methods of mechanical harvesting and related technical innovations are necessary in the sour cherry verticum. A key question is the effectiveness of phytosanitary procedures with special reference to the Monilia fungus and to the cherry fruit fly as the most important pest. There are two points of break through in the Hungarian sour cherry verticum. On the one hand, meeting the increasing demands in fuits for fresh consumption, on the other hand, the diversification of processed sour cherry products and their introduction to the markets. Both are aiming to increase the competitiveness of the Hungarian sour cherry. For that purpose, outstanding varieties and excellent as well as internationally recognised fruit qualities are ready to be utilized. The most susceptible problems of the Hungarian sour cherry verticum are associated with marketing, alliance of the grower-and processor organisations and their co-operation because no overall integration within the sour cherry verticum has been established yet. Most urgent necessity as well as possibility of changes are felt in the Northern Great Plain Region.
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Jerusalem artichoke (Helianthus tuberosus L.): A review of in vivo and in vitro propagation
131-136.Views:490Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement. It is a perennial tuberous plant rich in inulin and is a potential energy crop. During food shortages in times of war Jerusalem artichoke received more attention by scientists and farmers because of its multiple uses as a vegetable, medicinal plant, forage plant and source for biofuel. The energy crisis of the 1970s motivated research on Jerusalem artichoke for biofuel as the aboveground plant biomass and the tubers can be used for this purpose. There are different methods to propagate Jerusalem artichoke using tubers, rhizomes, slips (transplants derived from sprouted tubers), stem cuttings, seeds and tissue culture. So, this review was presented to highlight on propagation of Jerusalem artichoke via in vivo and in vitro techniques.
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Comparative study of sour cherry samples for their anthocyanin content measured by tools of the laboratory and by portable equipments
29-31.Views:246The inner quality of fruits depends on many components. The analysis of pharmacologically important fruit species as the sour cherry consumed as a common food is an expensive task, mostly requiring an equipped laboratory. In sour cherry, one of its valuable components, the anthocyanin content deserves special attention. A portable analytical implement, which has been developed by our team measures the anthocyanin content on a scale of 1024 PharMgrades. The system is a member of the UVEX family of implements working with a microprocessor and performs the measurement quickly. The portable variant (UVEX-ML-1) does not require much training, the laboratory variant (UVEX UL-1) performs a large quantity of tests under modest laboratory conditions. Preparation of the samples is easily done, the necessary elements are available in the trade. The reagent is easily stored and dosed. The system was tested and proved to be of sufficient precision and the result showed acceptable variance according to the checks performed by a spectrophotometer in the laboratory.
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Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures
15-23.Views:157The effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.
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Effect of hydroponic and peat-free media in transplant production of Rudbeckia hirta varieties under different photoperiodic lighting and their photosynthetic parameters
110-117Views:366The purpose of this research was to determine the effects of varieties, different light conditions (short day, long day, natural short day with light pollution), and different growing media (perlite, peat-free, peat-based, aeroponics system) on Rudbeckia hirta plant production under controlled conditions (greenhouse). The morphological effects of each treatment (photoperiodic lightings and media) on different Rudbeckia varieties determined at 11 weeks-old ’Napfény’, ’Toto Gold’, ’Autumn Colors’, ’Prairie Sun’ and 16 weeks-old ’Napfény’. Plantlets received 12 hours daylight did not initiate flowers, remained stage of the leaf rosette in case of all varieties. The 14 hours light treatment in the aeroponics system and the same treatment in perlite and control (natural short day with 14 hours light pollution) plantlets had developed inflorescences or flower buds. The inflorescence axis of ‘Napfény’ was appeared at 13 weeks under long-day conditions, with 1.7 (perlite) - 2.7 (aeroponics) flower buds in 16 weeks. ’Toto Gold’, ’Autumn Colors’, ’Prairie Sun’ varieties developed inflorescences at 8 weeks, 14 hours aeroponics system resulted in the most of flower buds (’Toto Gold’: 6.5, ’Autumn Colors’: 3.25,’Prairie Sun’: 4.8 flower buds) at 11 weeks. Long daylight manipulation could be minimized crop times and achieved flowering potted plants at 11 weeks. The peat-based and peat-free media effect was observed on ‘Autumn Colors’. The number of leaves of peat-free ‘Autumn Colors’ transplants (16.8-20.3) was significantly higher than peat-based media (13.5-15.5). Other morphological parameters were not affected by the media treatments.
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In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants
15-20.Views:364Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.
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Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation
30-38.Views:311Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.